Enterococcus sp. 10A9_DIV0425

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; unclassified Enterococcus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2644 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A242JY31|A0A242JY31_9ENTE Uncharacterized protein OS=Enterococcus sp. 10A9_DIV0425 OX=1987383 GN=A5844_001726 PE=4 SV=1
MM1 pKa = 7.64IKK3 pKa = 9.52GTFKK7 pKa = 11.03RR8 pKa = 11.84NDD10 pKa = 3.09AGQIVSFTLTGHH22 pKa = 7.19ADD24 pKa = 3.37AGPYY28 pKa = 10.55GSDD31 pKa = 2.94IVCAGVSALAISTVNGIASLAGFEE55 pKa = 5.35PIVDD59 pKa = 3.87MNEE62 pKa = 3.56EE63 pKa = 4.34DD64 pKa = 3.46GGYY67 pKa = 10.11LYY69 pKa = 11.19VEE71 pKa = 4.49ITSGMTQEE79 pKa = 4.13QNNIAEE85 pKa = 4.61ILLEE89 pKa = 4.2NCLLGLQSIEE99 pKa = 4.5AEE101 pKa = 3.73NSEE104 pKa = 4.56YY105 pKa = 10.52IQIEE109 pKa = 4.36TTNDD113 pKa = 3.03KK114 pKa = 11.22

Molecular weight: 12.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A242JZL3|A0A242JZL3_9ENTE Uncharacterized protein OS=Enterococcus sp. 10A9_DIV0425 OX=1987383 GN=A5844_002071 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84QKK14 pKa = 9.38VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.73GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2644

0

2644

877332

19

3845

331.8

37.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.553 ± 0.05

0.625 ± 0.013

5.009 ± 0.038

7.717 ± 0.06

4.597 ± 0.039

6.177 ± 0.049

1.897 ± 0.02

7.58 ± 0.051

7.214 ± 0.053

9.819 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.601 ± 0.027

4.66 ± 0.037

3.564 ± 0.028

4.497 ± 0.038

4.013 ± 0.036

6.111 ± 0.038

6.14 ± 0.04

6.524 ± 0.039

1.0 ± 0.018

3.701 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski