Streptomyces phage Lannister

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Likavirus; Streptomyces virus Lannister

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1Y9V9|A0A0K1Y9V9_9CAUD Uncharacterized protein OS=Streptomyces phage Lannister OX=1674927 GN=SEA_LANNISTER_1 PE=4 SV=1
MM1 pKa = 7.22NVDD4 pKa = 2.8IDD6 pKa = 4.35TVQEE10 pKa = 4.09YY11 pKa = 11.2ADD13 pKa = 3.75NALEE17 pKa = 4.36RR18 pKa = 11.84PSDD21 pKa = 3.4AAFWDD26 pKa = 3.92SRR28 pKa = 11.84CYY30 pKa = 7.57EE31 pKa = 3.89THH33 pKa = 5.99VPVIGWADD41 pKa = 3.62RR42 pKa = 11.84GDD44 pKa = 4.95DD45 pKa = 3.38ILEE48 pKa = 4.06EE49 pKa = 4.48SNYY52 pKa = 10.26HH53 pKa = 4.92SAKK56 pKa = 10.63SLIEE60 pKa = 4.15GAATDD65 pKa = 4.12PEE67 pKa = 4.7HH68 pKa = 7.97VFEE71 pKa = 5.15GSAGHH76 pKa = 6.45WLVGSLSQVWVQVYY90 pKa = 7.04EE91 pKa = 4.16TRR93 pKa = 11.84PEE95 pKa = 4.19CDD97 pKa = 2.83TIGCEE102 pKa = 4.11EE103 pKa = 4.49EE104 pKa = 4.01ATEE107 pKa = 4.19VASYY111 pKa = 10.11TRR113 pKa = 11.84TSGEE117 pKa = 3.78GHH119 pKa = 6.7FCRR122 pKa = 11.84DD123 pKa = 2.92HH124 pKa = 7.18ALPLLYY130 pKa = 10.7SPIGEE135 pKa = 4.19LLGVEE140 pKa = 5.14LEE142 pKa = 4.1DD143 pKa = 4.73LPRR146 pKa = 11.84EE147 pKa = 3.9FTAAFIEE154 pKa = 4.3AVEE157 pKa = 4.07IQEE160 pKa = 4.21ALKK163 pKa = 10.44DD164 pKa = 3.87YY165 pKa = 10.57PIVDD169 pKa = 3.39EE170 pKa = 4.73SDD172 pKa = 3.15FSEE175 pKa = 4.7RR176 pKa = 11.84EE177 pKa = 3.71WVRR180 pKa = 11.84FEE182 pKa = 4.51EE183 pKa = 4.26NCKK186 pKa = 10.07EE187 pKa = 4.15ALEE190 pKa = 4.19QAQRR194 pKa = 11.84EE195 pKa = 4.29YY196 pKa = 11.21EE197 pKa = 4.15DD198 pKa = 5.73DD199 pKa = 3.83SVEE202 pKa = 4.74DD203 pKa = 3.39EE204 pKa = 4.46TEE206 pKa = 3.51IEE208 pKa = 3.83NRR210 pKa = 11.84IFSEE214 pKa = 4.31SALSDD219 pKa = 3.54LFGYY223 pKa = 7.72EE224 pKa = 3.92ANAEE228 pKa = 4.24VSWEE232 pKa = 4.2SVAEE236 pKa = 4.27IYY238 pKa = 10.84AEE240 pKa = 3.9YY241 pKa = 10.36RR242 pKa = 11.84DD243 pKa = 4.18AYY245 pKa = 9.72FLEE248 pKa = 4.93RR249 pKa = 11.84ATEE252 pKa = 4.16VYY254 pKa = 9.45RR255 pKa = 11.84WNVLGYY261 pKa = 10.83NPDD264 pKa = 3.47QLEE267 pKa = 4.22LDD269 pKa = 3.4IVIIVAAA276 pKa = 4.47

Molecular weight: 31.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1Y9B5|A0A0K1Y9B5_9CAUD Tail assembly chaperone OS=Streptomyces phage Lannister OX=1674927 GN=SEA_LANNISTER_18 PE=4 SV=1
MM1 pKa = 7.22SVQRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84GQVARR12 pKa = 11.84IWKK15 pKa = 4.65TTKK18 pKa = 10.72VIDD21 pKa = 3.44NRR23 pKa = 11.84GNEE26 pKa = 3.77VHH28 pKa = 6.31VAHH31 pKa = 7.12NDD33 pKa = 3.45GPHH36 pKa = 4.82VVRR39 pKa = 11.84AAFIPQRR46 pKa = 11.84SAKK49 pKa = 10.63AEE51 pKa = 4.13VPGQMQINITRR62 pKa = 11.84MIVAADD68 pKa = 3.77LEE70 pKa = 4.88GVEE73 pKa = 4.06LWSRR77 pKa = 11.84VEE79 pKa = 4.01WNGKK83 pKa = 6.9QWDD86 pKa = 4.06IVTPPAYY93 pKa = 10.01HH94 pKa = 6.38HH95 pKa = 6.93GSRR98 pKa = 11.84QTRR101 pKa = 11.84HH102 pKa = 4.11WSIDD106 pKa = 2.86IRR108 pKa = 11.84EE109 pKa = 4.09RR110 pKa = 11.84PP111 pKa = 3.36

Molecular weight: 12.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

15216

43

1159

208.4

22.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.555 ± 0.3

0.69 ± 0.117

6.342 ± 0.225

7.249 ± 0.483

3.273 ± 0.165

8.465 ± 0.391

2.018 ± 0.194

4.397 ± 0.334

4.403 ± 0.276

8.498 ± 0.383

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.064 ± 0.139

3.023 ± 0.137

4.896 ± 0.219

3.293 ± 0.147

6.467 ± 0.368

5.948 ± 0.256

6.513 ± 0.358

7.302 ± 0.235

1.88 ± 0.145

2.727 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski