Streptomyces phage Xkcd426

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Woodruffvirus; unclassified Woodruffvirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A125RNU8|A0A125RNU8_9CAUD Uncharacterized protein OS=Streptomyces phage Xkcd426 OX=1784947 GN=SEA_XKCD426_19 PE=4 SV=1
MM1 pKa = 7.8IIQYY5 pKa = 10.31NYY7 pKa = 10.71NPDD10 pKa = 2.99GSIASTVLLNATTGTPYY27 pKa = 10.4GAPVGEE33 pKa = 4.15ITVCPTDD40 pKa = 2.82IGQPDD45 pKa = 3.96RR46 pKa = 11.84DD47 pKa = 3.93MQVLCDD53 pKa = 3.69VQANGTQVPIVRR65 pKa = 11.84DD66 pKa = 3.59YY67 pKa = 11.71VRR69 pKa = 11.84DD70 pKa = 3.6PNGIIISFNDD80 pKa = 3.27YY81 pKa = 9.07TVNGVSYY88 pKa = 8.69TATGTVGSCIPRR100 pKa = 11.84DD101 pKa = 3.68VEE103 pKa = 4.47TVILCDD109 pKa = 4.12SAATPNRR116 pKa = 11.84FLRR119 pKa = 11.84TYY121 pKa = 9.7TYY123 pKa = 10.64GSTGAISGFTDD134 pKa = 3.21TTLAGAPFTPTGAVNNCVVTTATDD158 pKa = 3.18QDD160 pKa = 3.89FLEE163 pKa = 4.87EE164 pKa = 4.18VLCDD168 pKa = 3.86GNGTPFIRR176 pKa = 11.84RR177 pKa = 11.84FTFNSQTGAVISTTNLTLAGTAFTPVGTVGTCTGCCPVVMGEE219 pKa = 4.26GCTNTGSGRR228 pKa = 11.84YY229 pKa = 6.19TALRR233 pKa = 11.84LANGTISLIDD243 pKa = 3.95SVTGTAVTQAQIIACAGDD261 pKa = 3.63GGTSGVTLTAEE272 pKa = 4.02ARR274 pKa = 11.84TVTNGTPWTPGADD287 pKa = 3.39VTGTLTSVTVTGTVGLWDD305 pKa = 3.61MVDD308 pKa = 3.53ANGTALTGLPPGLTLTWSALDD329 pKa = 3.87GNTLTGPQSITPQTGSTVVVNWTRR353 pKa = 11.84KK354 pKa = 8.53

Molecular weight: 36.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A125RP00|A0A125RP00_9CAUD Uncharacterized protein OS=Streptomyces phage Xkcd426 OX=1784947 GN=SEA_XKCD426_71 PE=4 SV=1
MM1 pKa = 7.79AGALPRR7 pKa = 11.84NRR9 pKa = 11.84GNRR12 pKa = 11.84SANRR16 pKa = 11.84TGATVSRR23 pKa = 11.84RR24 pKa = 11.84LRR26 pKa = 11.84AADD29 pKa = 3.73HH30 pKa = 6.29NVVSPDD36 pKa = 3.12RR37 pKa = 11.84RR38 pKa = 11.84HH39 pKa = 6.49KK40 pKa = 10.14SFCMTVRR47 pKa = 11.84AAGDD51 pKa = 3.78HH52 pKa = 5.98VSVLVDD58 pKa = 3.03YY59 pKa = 9.2GIPSKK64 pKa = 9.28NQRR67 pKa = 11.84VAEE70 pKa = 4.22DD71 pKa = 3.21VAATLRR77 pKa = 11.84GWSQVSDD84 pKa = 4.18LDD86 pKa = 3.54VSPIVDD92 pKa = 3.68DD93 pKa = 4.43AEE95 pKa = 4.26GAVFVRR101 pKa = 11.84FTYY104 pKa = 10.37TPTSRR109 pKa = 11.84QKK111 pKa = 11.06

Molecular weight: 12.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

19514

40

1178

250.2

26.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.842 ± 0.533

1.681 ± 0.255

6.565 ± 0.276

4.884 ± 0.264

2.685 ± 0.157

9.378 ± 0.505

1.876 ± 0.174

3.654 ± 0.251

2.88 ± 0.242

7.938 ± 0.3

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.147 ± 0.135

3.054 ± 0.202

5.786 ± 0.29

3.444 ± 0.161

6.708 ± 0.312

5.094 ± 0.159

7.759 ± 0.339

8.04 ± 0.211

1.548 ± 0.1

2.04 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski