Icoaraci virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Icoaraci phlebovirus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8D7V5|A0A4P8D7V5_9VIRU Glycoprotein OS=Icoaraci virus OX=426790 PE=4 SV=1
MM1 pKa = 7.59NIMNFIYY8 pKa = 10.03EE9 pKa = 4.07MPIILSYY16 pKa = 11.41GGMGNPEE23 pKa = 4.38AEE25 pKa = 4.2VGYY28 pKa = 10.43YY29 pKa = 9.77PYY31 pKa = 10.96EE32 pKa = 4.1GFTTYY37 pKa = 10.03RR38 pKa = 11.84VSVHH42 pKa = 6.83HH43 pKa = 7.21DD44 pKa = 3.12MEE46 pKa = 6.03IPVGTYY52 pKa = 9.44RR53 pKa = 11.84QVMDD57 pKa = 3.89HH58 pKa = 7.22RR59 pKa = 11.84STLEE63 pKa = 3.67DD64 pKa = 4.51FYY66 pKa = 11.75SRR68 pKa = 11.84GHH70 pKa = 6.38FPFRR74 pKa = 11.84WGPGSFNSRR83 pKa = 11.84VEE85 pKa = 4.12NPTCSDD91 pKa = 3.24FDD93 pKa = 3.76EE94 pKa = 4.45MLRR97 pKa = 11.84EE98 pKa = 4.45LMLFPNEE105 pKa = 3.7AYY107 pKa = 8.84TRR109 pKa = 11.84SFLPNISEE117 pKa = 4.66AISWPLGYY125 pKa = 9.35PCSKK129 pKa = 10.49FIQLSNEE136 pKa = 4.07QKK138 pKa = 10.33SQLTLRR144 pKa = 11.84SWKK147 pKa = 10.1SAAATCILRR156 pKa = 11.84MNPWVSTFDD165 pKa = 3.38QAFVEE170 pKa = 4.12AHH172 pKa = 6.3KK173 pKa = 10.44MILKK177 pKa = 9.46EE178 pKa = 3.82ATNRR182 pKa = 11.84GLSRR186 pKa = 11.84MLFPGYY192 pKa = 10.72DD193 pKa = 3.77LIKK196 pKa = 10.41EE197 pKa = 4.17VAIVQLVRR205 pKa = 11.84AMNALDD211 pKa = 3.61IDD213 pKa = 4.04MTYY216 pKa = 11.12SRR218 pKa = 11.84IPTVLTNMLEE228 pKa = 3.76KK229 pKa = 10.49HH230 pKa = 6.19KK231 pKa = 11.17NVFEE235 pKa = 4.21NEE237 pKa = 3.91NPDD240 pKa = 3.86LLGNRR245 pKa = 11.84KK246 pKa = 8.96WIPSDD251 pKa = 3.52DD252 pKa = 3.23TWFEE256 pKa = 3.72VLNYY260 pKa = 10.0HH261 pKa = 6.78GEE263 pKa = 4.42SEE265 pKa = 4.81FDD267 pKa = 3.26TSSDD271 pKa = 2.82SDD273 pKa = 3.7AAA275 pKa = 3.95

Molecular weight: 31.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8D7V8|A0A4P8D7V8_9VIRU Nucleoprotein OS=Icoaraci virus OX=426790 PE=3 SV=1
MM1 pKa = 7.54TDD3 pKa = 3.26YY4 pKa = 11.58ARR6 pKa = 11.84IAVEE10 pKa = 4.26FSGEE14 pKa = 3.98AVNLAEE20 pKa = 4.11IQGWVADD27 pKa = 4.23FAYY30 pKa = 10.43QGFDD34 pKa = 2.65ARR36 pKa = 11.84RR37 pKa = 11.84IVEE40 pKa = 4.28LVQQKK45 pKa = 10.65GGATWKK51 pKa = 10.63DD52 pKa = 3.68DD53 pKa = 3.7VKK55 pKa = 11.23MMIVLCLTRR64 pKa = 11.84GNKK67 pKa = 5.97PTKK70 pKa = 9.31MVEE73 pKa = 3.9KK74 pKa = 9.51MSPEE78 pKa = 3.86GKK80 pKa = 10.01VKK82 pKa = 10.44VNRR85 pKa = 11.84LISTYY90 pKa = 9.01GLKK93 pKa = 10.39SGNPGRR99 pKa = 11.84DD100 pKa = 3.8DD101 pKa = 3.22ITLSRR106 pKa = 11.84VAAAFAGWTCQALNVLHH123 pKa = 6.48PHH125 pKa = 6.59LPVSGTTMDD134 pKa = 5.36AISPNYY140 pKa = 8.24PRR142 pKa = 11.84AMMHH146 pKa = 6.41PCFAGLVDD154 pKa = 3.47PTIPAEE160 pKa = 4.04YY161 pKa = 10.1CQMIVDD167 pKa = 4.26AMSVFLIQFSRR178 pKa = 11.84TINKK182 pKa = 9.32SLRR185 pKa = 11.84GQPKK189 pKa = 7.92EE190 pKa = 3.77AVVEE194 pKa = 4.45SFVQPMQAAMASSFITPAEE213 pKa = 3.96RR214 pKa = 11.84RR215 pKa = 11.84KK216 pKa = 10.83LMIALGIVDD225 pKa = 5.03ANGKK229 pKa = 9.38PSANVAAAAAAYY241 pKa = 7.94PKK243 pKa = 10.63VII245 pKa = 4.17

Molecular weight: 26.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3958

245

2084

989.5

111.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.584 ± 0.996

2.501 ± 0.79

5.508 ± 0.378

6.998 ± 0.627

4.219 ± 0.672

6.443 ± 0.644

2.35 ± 0.197

6.064 ± 0.117

6.771 ± 0.754

8.59 ± 0.486

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.487 ± 0.473

4.169 ± 0.413

4.548 ± 0.404

3.032 ± 0.156

5.154 ± 0.111

8.818 ± 0.618

5.104 ± 0.065

6.695 ± 0.425

1.289 ± 0.168

2.678 ± 0.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski