Sphingomonas indica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Allosphingosinicella

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2739 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7GD15|A0A1X7GD15_9SPHN 50S ribosomal protein L4 OS=Sphingomonas indica OX=941907 GN=rplD PE=3 SV=1
MM1 pKa = 7.43VGTLKK6 pKa = 10.72DD7 pKa = 3.26IEE9 pKa = 4.43FEE11 pKa = 4.26GDD13 pKa = 2.88RR14 pKa = 11.84STRR17 pKa = 11.84DD18 pKa = 3.3LNNEE22 pKa = 3.57YY23 pKa = 11.0SDD25 pKa = 4.57AYY27 pKa = 9.47VAAVRR32 pKa = 11.84ASQANVDD39 pKa = 4.06GQGGTIRR46 pKa = 11.84GKK48 pKa = 9.58PVFTKK53 pKa = 10.91EE54 pKa = 3.49EE55 pKa = 3.57VAYY58 pKa = 9.95YY59 pKa = 10.51LNRR62 pKa = 11.84GDD64 pKa = 4.38GIIGGFEE71 pKa = 4.52SGANWDD77 pKa = 3.83GAQGYY82 pKa = 10.75ANNQYY87 pKa = 10.46YY88 pKa = 9.07WLPVTKK94 pKa = 10.68AMGGEE99 pKa = 3.87AGLPSEE105 pKa = 5.11GGPGATGPLTTLTFGFYY122 pKa = 7.63EE123 pKa = 4.4TLASLPEE130 pKa = 4.21PYY132 pKa = 10.24VYY134 pKa = 9.03RR135 pKa = 11.84TPAGNLALGLSVAQGFSPFTAAQRR159 pKa = 11.84TATRR163 pKa = 11.84EE164 pKa = 4.08AIGLWDD170 pKa = 6.15DD171 pKa = 3.95IIKK174 pKa = 10.87VNFQEE179 pKa = 4.84TSFQQGDD186 pKa = 4.26LNFMNTTTGPAQAAAYY202 pKa = 9.78LPYY205 pKa = 10.39DD206 pKa = 3.76YY207 pKa = 11.2GSEE210 pKa = 3.78EE211 pKa = 3.96GFKK214 pKa = 10.88NFEE217 pKa = 4.31GDD219 pKa = 3.37DD220 pKa = 3.46VSYY223 pKa = 11.51YY224 pKa = 10.52EE225 pKa = 3.74IAGDD229 pKa = 3.72VFVNPNQASNFQFLPGQYY247 pKa = 10.83GLNTLVHH254 pKa = 6.98EE255 pKa = 4.85IGHH258 pKa = 6.83AIGLEE263 pKa = 4.03HH264 pKa = 7.18PGAYY268 pKa = 10.25NFGPGFDD275 pKa = 3.33VTYY278 pKa = 11.0DD279 pKa = 3.31NGAEE283 pKa = 4.15YY284 pKa = 10.88YY285 pKa = 9.8QDD287 pKa = 2.89SRR289 pKa = 11.84MYY291 pKa = 10.86SIMSYY296 pKa = 9.7WDD298 pKa = 3.44AEE300 pKa = 4.43EE301 pKa = 3.96TGAAHH306 pKa = 6.68VNWEE310 pKa = 4.07NLTYY314 pKa = 10.38SYY316 pKa = 11.38NSTPMIHH323 pKa = 7.7DD324 pKa = 3.66ILAAQRR330 pKa = 11.84IYY332 pKa = 10.86GVEE335 pKa = 3.91EE336 pKa = 3.59NTRR339 pKa = 11.84TGNTTYY345 pKa = 11.09GFNSNAGKK353 pKa = 10.52DD354 pKa = 3.45MYY356 pKa = 11.4DD357 pKa = 3.23FTKK360 pKa = 10.8NPNPVISIYY369 pKa = 10.65DD370 pKa = 3.29AGGNDD375 pKa = 3.59TLDD378 pKa = 3.31LSGYY382 pKa = 6.28NTRR385 pKa = 11.84SFIDD389 pKa = 3.82LNPGAFSSAGGFYY402 pKa = 10.64SEE404 pKa = 6.3DD405 pKa = 3.43IPTLDD410 pKa = 4.11EE411 pKa = 4.14INARR415 pKa = 11.84RR416 pKa = 11.84AAAGLAPRR424 pKa = 11.84TQATYY429 pKa = 11.23DD430 pKa = 3.5LYY432 pKa = 10.77IEE434 pKa = 5.14LFGATYY440 pKa = 10.46TDD442 pKa = 3.24GLMRR446 pKa = 11.84DD447 pKa = 4.41NISIAYY453 pKa = 9.83NSIIEE458 pKa = 4.13NAVGGGGDD466 pKa = 3.67DD467 pKa = 3.62TIVANGAANRR477 pKa = 11.84IDD479 pKa = 3.83GGAGNDD485 pKa = 2.92IVSYY489 pKa = 8.05QTSGTAVSVNLANGLVSGGAAGDD512 pKa = 3.56VLISIEE518 pKa = 4.13GVTGSRR524 pKa = 11.84HH525 pKa = 5.53NDD527 pKa = 2.86VLTGSDD533 pKa = 3.75GNNVLNGGAGNDD545 pKa = 3.42IIYY548 pKa = 10.2GRR550 pKa = 11.84NGDD553 pKa = 3.77DD554 pKa = 4.28TIDD557 pKa = 3.86GGDD560 pKa = 3.88GVDD563 pKa = 3.81QLFGDD568 pKa = 4.82GGNDD572 pKa = 3.81TIRR575 pKa = 11.84GGDD578 pKa = 3.68GNDD581 pKa = 3.69TIMGGAGDD589 pKa = 5.58DD590 pKa = 3.92ILFGDD595 pKa = 4.76AGNDD599 pKa = 3.48TLYY602 pKa = 11.38GDD604 pKa = 4.75AGNDD608 pKa = 3.57VLDD611 pKa = 4.97GGAGDD616 pKa = 4.06DD617 pKa = 4.12KK618 pKa = 11.52LYY620 pKa = 11.25GGAGADD626 pKa = 3.3TFRR629 pKa = 11.84FSDD632 pKa = 3.44LGGYY636 pKa = 7.53DD637 pKa = 3.41TIVDD641 pKa = 4.24FRR643 pKa = 11.84RR644 pKa = 11.84GQGDD648 pKa = 3.92KK649 pKa = 10.48IDD651 pKa = 3.94LTGIDD656 pKa = 3.78PNINVDD662 pKa = 3.62AVNEE666 pKa = 3.94QFNFIGNAAFSGTAGEE682 pKa = 4.54LRR684 pKa = 11.84TFVDD688 pKa = 3.4RR689 pKa = 11.84GSTIIAGDD697 pKa = 3.61VNGDD701 pKa = 3.39GVADD705 pKa = 3.93FQINVGATAIRR716 pKa = 11.84ADD718 pKa = 4.1DD719 pKa = 5.24LILTVNVAAA728 pKa = 5.4

Molecular weight: 76.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7FZY8|A0A1X7FZY8_9SPHN Phosphoribosylformylglycinamidine synthase subunit PurL OS=Sphingomonas indica OX=941907 GN=purL PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84TRR21 pKa = 11.84SATVGGRR28 pKa = 11.84KK29 pKa = 9.07VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2739

0

2739

862315

32

3070

314.8

33.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.203 ± 0.073

0.719 ± 0.013

6.132 ± 0.037

5.595 ± 0.04

3.467 ± 0.031

9.095 ± 0.06

1.866 ± 0.024

4.962 ± 0.033

2.937 ± 0.035

9.756 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.346 ± 0.027

2.362 ± 0.03

5.447 ± 0.039

2.878 ± 0.028

7.71 ± 0.053

4.867 ± 0.034

5.019 ± 0.038

7.098 ± 0.037

1.377 ± 0.017

2.163 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski