Faeces associated gemycircularvirus 20

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus chicas1; Chicken associated gemycircularvirus 1

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A161J5N8|A0A161J5N8_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 20 OX=1843740 PE=3 SV=1
MM1 pKa = 7.65SAFQVNSRR9 pKa = 11.84YY10 pKa = 10.26VLLTYY15 pKa = 9.15AQCGDD20 pKa = 4.04LDD22 pKa = 3.68PWAVNDD28 pKa = 4.49LLSTLGAEE36 pKa = 4.61CIIGRR41 pKa = 11.84EE42 pKa = 3.82RR43 pKa = 11.84HH44 pKa = 5.63EE45 pKa = 5.1DD46 pKa = 3.44GGIHH50 pKa = 6.34LHH52 pKa = 6.78AFVDD56 pKa = 4.89FNRR59 pKa = 11.84KK60 pKa = 8.37FRR62 pKa = 11.84TRR64 pKa = 11.84RR65 pKa = 11.84SDD67 pKa = 2.97IFDD70 pKa = 3.44VDD72 pKa = 3.4GHH74 pKa = 6.68HH75 pKa = 7.16PNISQSRR82 pKa = 11.84GTPEE86 pKa = 3.51KK87 pKa = 10.76GYY89 pKa = 10.89DD90 pKa = 3.47YY91 pKa = 10.69AIKK94 pKa = 10.87DD95 pKa = 3.59GDD97 pKa = 4.04VVAGGLGRR105 pKa = 11.84PEE107 pKa = 4.32GKK109 pKa = 10.05SGGGDD114 pKa = 3.2GSTHH118 pKa = 6.5AKK120 pKa = 6.66WTAITQAEE128 pKa = 4.18NRR130 pKa = 11.84EE131 pKa = 4.28QFWEE135 pKa = 4.49LCHH138 pKa = 6.74EE139 pKa = 5.29LDD141 pKa = 4.04PKK143 pKa = 10.91AAATSFTQLSKK154 pKa = 9.94YY155 pKa = 10.47ADD157 pKa = 3.09WRR159 pKa = 11.84FAPDD163 pKa = 3.51PPVYY167 pKa = 9.44EE168 pKa = 4.16HH169 pKa = 7.59PIGISFTDD177 pKa = 3.42GDD179 pKa = 4.13LDD181 pKa = 5.01GRR183 pKa = 11.84RR184 pKa = 11.84EE185 pKa = 3.98WLDD188 pKa = 3.12QAGIGGGEE196 pKa = 3.44RR197 pKa = 11.84RR198 pKa = 11.84GRR200 pKa = 11.84CKK202 pKa = 10.41SLCLFGRR209 pKa = 11.84SRR211 pKa = 11.84TGKK214 pKa = 7.28TLWARR219 pKa = 11.84SLGQHH224 pKa = 6.7IYY226 pKa = 10.77CVGLVSGDD234 pKa = 3.67EE235 pKa = 4.25CMKK238 pKa = 11.14APDD241 pKa = 3.23VDD243 pKa = 3.92YY244 pKa = 11.36AIFDD248 pKa = 4.95DD249 pKa = 4.22IRR251 pKa = 11.84GGMKK255 pKa = 9.69FFPSFKK261 pKa = 9.87EE262 pKa = 3.78WLGAQAWVTVKK273 pKa = 10.56RR274 pKa = 11.84LYY276 pKa = 10.53RR277 pKa = 11.84EE278 pKa = 3.84PALVQWGKK286 pKa = 10.09PSIWLANSDD295 pKa = 3.98PRR297 pKa = 11.84NDD299 pKa = 3.35MSQDD303 pKa = 3.15DD304 pKa = 4.55VQWMEE309 pKa = 4.4DD310 pKa = 2.74NCIFVEE316 pKa = 4.32CNEE319 pKa = 4.42TIFRR323 pKa = 11.84ANIEE327 pKa = 4.03

Molecular weight: 36.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A161IHP1|A0A161IHP1_9VIRU RepA OS=Faeces associated gemycircularvirus 20 OX=1843740 PE=3 SV=1
MM1 pKa = 7.51PARR4 pKa = 11.84YY5 pKa = 6.58PTARR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84VVRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84PVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84YY24 pKa = 7.32PVRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84VFRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84STRR38 pKa = 11.84TTISRR43 pKa = 11.84RR44 pKa = 11.84AILNVASSKK53 pKa = 11.06KK54 pKa = 9.14KK55 pKa = 10.34DD56 pKa = 3.3NMVAFRR62 pKa = 11.84QTGSDD67 pKa = 3.77SEE69 pKa = 4.37PTQGPLTITGNPTLSAFGEE88 pKa = 4.67HH89 pKa = 5.59IVMWLPTARR98 pKa = 11.84NRR100 pKa = 11.84GTVPEE105 pKa = 4.08EE106 pKa = 4.06TEE108 pKa = 4.61RR109 pKa = 11.84NSQTCFMRR117 pKa = 11.84GLSEE121 pKa = 5.15RR122 pKa = 11.84INISTDD128 pKa = 2.9TSLPWTWRR136 pKa = 11.84RR137 pKa = 11.84ICFTLKK143 pKa = 9.99GLGVIRR149 pKa = 11.84RR150 pKa = 11.84DD151 pKa = 3.21GTAINVLSFEE161 pKa = 4.11EE162 pKa = 4.34AAPNGYY168 pKa = 9.27VRR170 pKa = 11.84LARR173 pKa = 11.84NYY175 pKa = 10.28SSVGGDD181 pKa = 3.81PNWEE185 pKa = 4.38AIAEE189 pKa = 4.04QLISLIMDD197 pKa = 4.02GTRR200 pKa = 11.84GIDD203 pKa = 3.95FLDD206 pKa = 3.66LTTAKK211 pKa = 9.97TDD213 pKa = 3.57GTNVTIKK220 pKa = 10.39YY221 pKa = 10.46DD222 pKa = 3.43RR223 pKa = 11.84TFRR226 pKa = 11.84LASGNDD232 pKa = 3.13SGIQKK237 pKa = 9.8VYY239 pKa = 10.95KK240 pKa = 10.0LWHH243 pKa = 6.39PMNKK247 pKa = 9.58NLVYY251 pKa = 10.83DD252 pKa = 4.4DD253 pKa = 5.8DD254 pKa = 4.96EE255 pKa = 7.3DD256 pKa = 5.7GGDD259 pKa = 3.35MVGNSISTQGKK270 pKa = 8.43PGMGDD275 pKa = 3.57YY276 pKa = 10.98YY277 pKa = 11.48VVDD280 pKa = 3.62IFKK283 pKa = 10.39AHH285 pKa = 6.95PAATSSDD292 pKa = 3.44HH293 pKa = 7.51LLFDD297 pKa = 5.29PMATLYY303 pKa = 9.48WHH305 pKa = 7.03EE306 pKa = 4.25KK307 pKa = 9.3

Molecular weight: 34.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

834

200

327

278.0

31.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.074 ± 0.387

1.679 ± 0.589

8.153 ± 0.712

5.156 ± 0.549

4.197 ± 0.449

9.832 ± 1.034

2.638 ± 0.466

5.276 ± 0.114

4.077 ± 0.108

6.595 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.799 ± 0.501

3.597 ± 0.559

4.676 ± 0.255

2.998 ± 0.477

9.113 ± 1.324

6.115 ± 0.315

6.235 ± 1.338

5.156 ± 0.455

2.638 ± 0.396

2.998 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski