Actinidia chlorotic ringspot-associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Fimoviridae; Emaravirus; Actinidia chlorotic ringspot-associated emaravirus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U4CCN7|A0A0U4CCN7_9VIRU Replicase OS=Actinidia chlorotic ringspot-associated virus OX=1776763 PE=4 SV=1
MM1 pKa = 7.58EE2 pKa = 5.56LFKK5 pKa = 10.56TYY7 pKa = 10.64LSAFSWMYY15 pKa = 9.87EE16 pKa = 3.99QIFHH20 pKa = 7.21IISEE24 pKa = 4.46EE25 pKa = 4.14FPSKK29 pKa = 11.07YY30 pKa = 10.27YY31 pKa = 10.66DD32 pKa = 3.31NCCIIHH38 pKa = 6.9EE39 pKa = 4.57YY40 pKa = 10.42YY41 pKa = 10.12MSGTIIYY48 pKa = 9.55EE49 pKa = 4.05DD50 pKa = 4.06LFFDD54 pKa = 4.84GPHH57 pKa = 5.76KK58 pKa = 10.54RR59 pKa = 11.84IYY61 pKa = 10.85DD62 pKa = 3.66DD63 pKa = 4.17VINAGSAAYY72 pKa = 9.6YY73 pKa = 10.39RR74 pKa = 11.84KK75 pKa = 9.54FQALCFMVLGHH86 pKa = 6.9LEE88 pKa = 4.12PLFKK92 pKa = 10.81KK93 pKa = 10.14HH94 pKa = 6.31GDD96 pKa = 3.63KK97 pKa = 11.17LDD99 pKa = 3.66GLQIRR104 pKa = 11.84DD105 pKa = 3.83YY106 pKa = 11.19SSHH109 pKa = 7.24LDD111 pKa = 3.21QGLCYY116 pKa = 9.48IRR118 pKa = 11.84HH119 pKa = 6.19IFRR122 pKa = 11.84GYY124 pKa = 10.9LMEE127 pKa = 4.7RR128 pKa = 11.84MNFEE132 pKa = 4.01MANQYY137 pKa = 10.39ALKK140 pKa = 10.69SFDD143 pKa = 3.25SKK145 pKa = 10.61MVYY148 pKa = 8.25GTPSFEE154 pKa = 4.63FYY156 pKa = 10.58SKK158 pKa = 10.98SIMYY162 pKa = 9.43LQLKK166 pKa = 9.4HH167 pKa = 6.63ISTISDD173 pKa = 3.53SLKK176 pKa = 11.1DD177 pKa = 3.54GLKK180 pKa = 10.27SLKK183 pKa = 10.95SNDD186 pKa = 4.0LSDD189 pKa = 5.72DD190 pKa = 3.81DD191 pKa = 5.64DD192 pKa = 4.65EE193 pKa = 6.55SGEE196 pKa = 4.05ILKK199 pKa = 10.74KK200 pKa = 10.79LLLDD204 pKa = 3.92NTDD207 pKa = 3.25QTASTSCKK215 pKa = 9.81LKK217 pKa = 10.35RR218 pKa = 11.84QISIISIKK226 pKa = 10.67

Molecular weight: 26.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4B558|A0A0U4B558_9VIRU Uncharacterized protein OS=Actinidia chlorotic ringspot-associated virus OX=1776763 PE=4 SV=1
MM1 pKa = 7.84PKK3 pKa = 10.16PMQGDD8 pKa = 3.79ASNSGSAVPSNVKK21 pKa = 9.68IDD23 pKa = 3.76PNTVKK28 pKa = 10.52FGKK31 pKa = 9.74KK32 pKa = 9.64NSVRR36 pKa = 11.84TITFTEE42 pKa = 4.45VVNNRR47 pKa = 11.84ATSSKK52 pKa = 10.37NSSNTAVSVFKK63 pKa = 11.14SPAFVGGFTSDD74 pKa = 3.71LDD76 pKa = 3.87FDD78 pKa = 3.45ITKK81 pKa = 9.57YY82 pKa = 10.77SRR84 pKa = 11.84VGSVEE89 pKa = 3.81TCASHH94 pKa = 7.32LSNSQEE100 pKa = 3.57IKK102 pKa = 10.41KK103 pKa = 10.46RR104 pKa = 11.84LVSDD108 pKa = 4.31GFLEE112 pKa = 4.79LPISNNYY119 pKa = 10.51SLFLCSEE126 pKa = 4.09IQATSIEE133 pKa = 4.26NAVSFNKK140 pKa = 10.1ACAIMAYY147 pKa = 9.9QILAHH152 pKa = 5.64TVMEE156 pKa = 4.61KK157 pKa = 10.87YY158 pKa = 10.3DD159 pKa = 3.54WEE161 pKa = 4.04QFKK164 pKa = 10.92AVEE167 pKa = 4.04VAIKK171 pKa = 10.21DD172 pKa = 3.63RR173 pKa = 11.84KK174 pKa = 9.74NPSISIVNRR183 pKa = 11.84LAGQMRR189 pKa = 11.84LPQSSLYY196 pKa = 10.04YY197 pKa = 8.86WMVVPGYY204 pKa = 10.09EE205 pKa = 3.77FLYY208 pKa = 10.67DD209 pKa = 3.42VFPIEE214 pKa = 4.45TIALTMVRR222 pKa = 11.84LEE224 pKa = 4.22CRR226 pKa = 11.84KK227 pKa = 10.04ALNIPEE233 pKa = 4.82TITNAEE239 pKa = 4.07IVNSLVSKK247 pKa = 9.54MNRR250 pKa = 11.84KK251 pKa = 9.02HH252 pKa = 6.93DD253 pKa = 3.78LTKK256 pKa = 10.82VSISEE261 pKa = 4.49IITKK265 pKa = 10.57LGVNNIKK272 pKa = 10.83DD273 pKa = 3.42MYY275 pKa = 11.09NEE277 pKa = 3.63FRR279 pKa = 11.84RR280 pKa = 11.84NVGTTGRR287 pKa = 11.84EE288 pKa = 3.6IRR290 pKa = 11.84NEE292 pKa = 3.88HH293 pKa = 5.89AVKK296 pKa = 10.51EE297 pKa = 4.01FLDD300 pKa = 5.32FIGKK304 pKa = 9.31YY305 pKa = 9.67CGSSTT310 pKa = 4.18

Molecular weight: 34.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3871

226

2303

774.2

89.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.591 ± 0.571

1.937 ± 0.5

6.768 ± 0.444

5.399 ± 0.231

4.805 ± 0.375

3.565 ± 0.403

2.532 ± 0.416

8.835 ± 0.465

8.06 ± 0.258

9.429 ± 0.96

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.867 ± 0.361

6.071 ± 0.539

3.229 ± 0.194

2.532 ± 0.285

3.565 ± 0.23

9.326 ± 0.409

5.761 ± 0.308

5.296 ± 1.066

0.491 ± 0.103

5.942 ± 0.681
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski