Spirobacillus cienkowskii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Oligoflexia; Silvanigrellales; Silvanigrellales incertae sedis; Spirobacillus

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2415 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A369KRI3|A0A369KRI3_9PROT ATP-dependent zinc metalloprotease FtsH OS=Spirobacillus cienkowskii OX=495820 GN=hflB PE=3 SV=1
MM1 pKa = 7.68KK2 pKa = 10.6NIDD5 pKa = 3.66QYY7 pKa = 12.26NLIKK11 pKa = 10.97NFIDD15 pKa = 3.64EE16 pKa = 4.81KK17 pKa = 10.59ISPGVLAHH25 pKa = 6.47GGEE28 pKa = 4.35VNIISLEE35 pKa = 4.04NNILTLEE42 pKa = 4.18LSGSCGSCSIQAYY55 pKa = 7.24TSEE58 pKa = 4.71SISNYY63 pKa = 9.31ILEE66 pKa = 4.87EE67 pKa = 4.29FPDD70 pKa = 4.49LDD72 pKa = 4.41DD73 pKa = 6.08VIVSDD78 pKa = 4.3

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A369KW62|A0A369KW62_9PROT ATP-dependent helicase OS=Spirobacillus cienkowskii OX=495820 GN=DCC88_01785 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNKK11 pKa = 8.51VRR13 pKa = 11.84KK14 pKa = 8.93RR15 pKa = 11.84KK16 pKa = 9.52HH17 pKa = 4.47GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.28NGRR29 pKa = 11.84NIIKK33 pKa = 10.2RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.88GRR40 pKa = 11.84SLLAATVYY48 pKa = 10.81VKK50 pKa = 10.96

Molecular weight: 5.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2415

0

2415

824229

27

3432

341.3

38.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.059 ± 0.055

1.21 ± 0.018

4.579 ± 0.029

6.3 ± 0.048

5.675 ± 0.046

5.289 ± 0.044

1.95 ± 0.025

8.805 ± 0.05

8.268 ± 0.049

9.972 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.961 ± 0.02

6.798 ± 0.061

3.431 ± 0.029

3.675 ± 0.03

3.502 ± 0.036

7.55 ± 0.047

4.923 ± 0.047

5.552 ± 0.04

0.945 ± 0.014

3.558 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski