Bacillus megaterium (strain ATCC 12872 / QMB1551)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia; Priestia megaterium

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5606 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5E0A7|D5E0A7_BACMQ Putative ABC transporter ATP-binding/permease protein OS=Bacillus megaterium (strain ATCC 12872 / QMB1551) OX=545693 GN=yknU PE=4 SV=1
MM1 pKa = 7.26PAIVGVVNLNSVGSASVFHH20 pKa = 7.57IGDD23 pKa = 3.67VYY25 pKa = 10.66TIAPISTAKK34 pKa = 9.6TFAGAGSFNTGDD46 pKa = 3.19GLYY49 pKa = 10.41VVNQQSSTNTSDD61 pKa = 5.09NDD63 pKa = 3.57VNDD66 pKa = 3.4QNVAGNLL73 pKa = 3.58

Molecular weight: 7.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5E0V4|D5E0V4_BACMQ Endoribonuclease L-PSP OS=Bacillus megaterium (strain ATCC 12872 / QMB1551) OX=545693 GN=BMQ_3591 PE=4 SV=1
MM1 pKa = 7.74HH2 pKa = 6.64ITAEE6 pKa = 4.35KK7 pKa = 9.92IKK9 pKa = 10.16EE10 pKa = 3.8AAEE13 pKa = 4.02ALEE16 pKa = 4.14STEE19 pKa = 4.59LKK21 pKa = 10.69SFMFQEE27 pKa = 4.39PTAGPLTRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84SSRR41 pKa = 11.84STGRR45 pKa = 11.84SRR47 pKa = 11.84RR48 pKa = 11.84STRR51 pKa = 11.84GTRR54 pKa = 11.84RR55 pKa = 11.84STRR58 pKa = 11.84GTRR61 pKa = 11.84RR62 pKa = 11.84STRR65 pKa = 11.84RR66 pKa = 11.84STRR69 pKa = 11.84GTRR72 pKa = 11.84RR73 pKa = 11.84STRR76 pKa = 11.84RR77 pKa = 11.84STRR80 pKa = 11.84GTRR83 pKa = 11.84RR84 pKa = 11.84STRR87 pKa = 11.84RR88 pKa = 11.84STRR91 pKa = 11.84GTRR94 pKa = 11.84RR95 pKa = 11.84STRR98 pKa = 11.84GTRR101 pKa = 11.84RR102 pKa = 11.84STRR105 pKa = 11.84GTRR108 pKa = 11.84RR109 pKa = 11.84STRR112 pKa = 11.84RR113 pKa = 11.84STRR116 pKa = 11.84GTRR119 pKa = 11.84RR120 pKa = 11.84STRR123 pKa = 11.84GTRR126 pKa = 11.84RR127 pKa = 11.84STRR130 pKa = 11.84GTRR133 pKa = 11.84RR134 pKa = 11.84STRR137 pKa = 11.84RR138 pKa = 11.84SRR140 pKa = 11.84RR141 pKa = 11.84STRR144 pKa = 11.84RR145 pKa = 11.84SRR147 pKa = 11.84RR148 pKa = 11.84STRR151 pKa = 11.84RR152 pKa = 11.84SRR154 pKa = 11.84RR155 pKa = 11.84STRR158 pKa = 11.84RR159 pKa = 11.84SRR161 pKa = 11.84RR162 pKa = 11.84STRR165 pKa = 11.84RR166 pKa = 11.84STRR169 pKa = 11.84RR170 pKa = 11.84SRR172 pKa = 11.84RR173 pKa = 11.84STRR176 pKa = 11.84RR177 pKa = 11.84SRR179 pKa = 11.84RR180 pKa = 11.84SRR182 pKa = 11.84RR183 pKa = 11.84STRR186 pKa = 11.84RR187 pKa = 11.84SRR189 pKa = 11.84RR190 pKa = 11.84STRR193 pKa = 11.84RR194 pKa = 11.84SS195 pKa = 3.07

Molecular weight: 23.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5606

0

5606

1495755

33

1517

266.8

29.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.168 ± 0.037

0.767 ± 0.009

4.741 ± 0.026

7.143 ± 0.046

4.573 ± 0.029

6.685 ± 0.035

2.269 ± 0.018

7.551 ± 0.032

7.16 ± 0.032

9.867 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.739 ± 0.017

4.234 ± 0.02

3.521 ± 0.02

4.005 ± 0.028

3.797 ± 0.026

6.354 ± 0.028

5.521 ± 0.023

7.269 ± 0.026

1.023 ± 0.013

3.613 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski