Synechococcus sp. WH 5701

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3316 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3Z0H3|A3Z0H3_9SYNE V-type ATP synthase alpha chain OS=Synechococcus sp. WH 5701 OX=69042 GN=atpA PE=3 SV=1
MM1 pKa = 7.59ALLRR5 pKa = 11.84KK6 pKa = 9.54AFQLWDD12 pKa = 3.55EE13 pKa = 4.41ALEE16 pKa = 4.19SVTFQEE22 pKa = 4.92VSPNDD27 pKa = 3.54GGAQIQVGLGEE38 pKa = 4.48TNGLSGYY45 pKa = 9.24WSATWSTVRR54 pKa = 11.84TSASITLAPNLDD66 pKa = 3.66PALFAVAAAHH76 pKa = 6.92EE77 pKa = 4.28IANVLGLGDD86 pKa = 3.97IDD88 pKa = 3.62GGGYY92 pKa = 9.51VEE94 pKa = 5.8SLTSDD99 pKa = 3.72PFSSFHH105 pKa = 6.86LSNPNLNYY113 pKa = 10.43LSRR116 pKa = 11.84IDD118 pKa = 4.96FEE120 pKa = 6.23LINQLYY126 pKa = 10.62GEE128 pKa = 5.31DD129 pKa = 3.51STDD132 pKa = 3.41YY133 pKa = 11.09LGYY136 pKa = 10.52LAGLSIFSQVIVGDD150 pKa = 4.22SNSALAEE157 pKa = 4.34SFSGTDD163 pKa = 3.09GADD166 pKa = 4.31LIRR169 pKa = 11.84GYY171 pKa = 11.12AGDD174 pKa = 3.79DD175 pKa = 3.5TLIGGSGNDD184 pKa = 4.69LIFAGDD190 pKa = 3.73GRR192 pKa = 11.84DD193 pKa = 4.01LIIGGDD199 pKa = 3.85GADD202 pKa = 3.43VMHH205 pKa = 7.04GGFGANIFGDD215 pKa = 3.89SRR217 pKa = 11.84DD218 pKa = 3.54GSVDD222 pKa = 3.32QILIHH227 pKa = 6.84SDD229 pKa = 3.15HH230 pKa = 6.86FLMNPLTGTTTDD242 pKa = 3.51NTSGEE247 pKa = 4.28YY248 pKa = 10.56VDD250 pKa = 4.69ILEE253 pKa = 5.15GLDD256 pKa = 3.38PSDD259 pKa = 4.75NIFIEE264 pKa = 4.41AGFGRR269 pKa = 11.84SLEE272 pKa = 4.15VRR274 pKa = 11.84DD275 pKa = 3.83TTGGLGLYY283 pKa = 10.06VDD285 pKa = 5.53GIIEE289 pKa = 4.3ALYY292 pKa = 9.8TGNDD296 pKa = 3.16LSAAQLFSMTEE307 pKa = 3.48IVAAA311 pKa = 4.38

Molecular weight: 32.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3Z0T2|A3Z0T2_9SYNE Glutamyl/Glutaminyl-tRNA synthetase OS=Synechococcus sp. WH 5701 OX=69042 GN=WH5701_06431 PE=3 SV=1
MM1 pKa = 7.56TKK3 pKa = 9.03RR4 pKa = 11.84TLEE7 pKa = 3.92GTSRR11 pKa = 11.84KK12 pKa = 9.24RR13 pKa = 11.84KK14 pKa = 7.95RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 6.21TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84TRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LAVV45 pKa = 3.5

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3316

0

3316

883610

19

1532

266.5

28.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.478 ± 0.052

1.134 ± 0.017

4.826 ± 0.032

5.934 ± 0.047

3.045 ± 0.029

8.774 ± 0.045

2.066 ± 0.025

3.925 ± 0.031

2.126 ± 0.03

13.231 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.904 ± 0.019

2.149 ± 0.025

6.21 ± 0.037

4.433 ± 0.031

7.73 ± 0.044

6.125 ± 0.034

4.426 ± 0.031

6.948 ± 0.04

1.757 ± 0.024

1.779 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski