Potato aucuba mosaic virus (PAMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q86823|Q86823_PAMV 12 kDa polypeptide protein OS=Potato aucuba mosaic virus OX=12182 GN=12 kda polypeptide PE=4 SV=1
MM1 pKa = 7.81EE2 pKa = 4.37YY3 pKa = 10.84SFLVRR8 pKa = 11.84LLDD11 pKa = 3.47HH12 pKa = 6.69YY13 pKa = 11.23GFEE16 pKa = 4.35RR17 pKa = 11.84TTEE20 pKa = 4.25KK21 pKa = 10.26IVPGQPIVVQGIAGCGKK38 pKa = 7.15TTLLRR43 pKa = 11.84NFHH46 pKa = 5.83QEE48 pKa = 3.94YY49 pKa = 9.37PSIPIYY55 pKa = 10.99SCFPQKK61 pKa = 10.28ISEE64 pKa = 4.15NSEE67 pKa = 3.8EE68 pKa = 4.11LQLLAKK74 pKa = 10.53ARR76 pKa = 11.84FTASAILDD84 pKa = 3.6EE85 pKa = 4.37YY86 pKa = 10.63LAHH89 pKa = 7.09KK90 pKa = 10.26FDD92 pKa = 4.03YY93 pKa = 10.2QKK95 pKa = 11.11CLAVFADD102 pKa = 4.07PLQYY106 pKa = 11.1SHH108 pKa = 7.24LGALRR113 pKa = 11.84PHH115 pKa = 6.12YY116 pKa = 8.5QTSKK120 pKa = 10.72HH121 pKa = 5.78IGLVLVLLILSLRR134 pKa = 11.84NWIPIEE140 pKa = 4.11SLLSEE145 pKa = 4.43EE146 pKa = 3.92KK147 pKa = 10.26TILKK151 pKa = 10.28EE152 pKa = 3.79CDD154 pKa = 3.53PYY156 pKa = 11.16ATDD159 pKa = 5.36PIGQIIASNHH169 pKa = 4.56EE170 pKa = 3.98VLNYY174 pKa = 9.85IKK176 pKa = 9.81PQAVEE181 pKa = 4.89AICSCEE187 pKa = 3.77VLGKK191 pKa = 10.18EE192 pKa = 4.33FQTVSCYY199 pKa = 9.51YY200 pKa = 9.99QSHH203 pKa = 6.55KK204 pKa = 11.19LEE206 pKa = 4.94DD207 pKa = 3.59SAEE210 pKa = 4.03EE211 pKa = 4.01RR212 pKa = 11.84RR213 pKa = 11.84GLYY216 pKa = 9.73IAISRR221 pKa = 11.84AKK223 pKa = 10.35SAVLLFDD230 pKa = 5.45LDD232 pKa = 3.7

Molecular weight: 26.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q86821|Q86821_PAMV Isoform of Q86824 8 kDa polypeptide protein OS=Potato aucuba mosaic virus OX=12182 GN=8 kda polypeptide PE=4 SV=1
MM1 pKa = 7.23TGKK4 pKa = 10.47SSTRR8 pKa = 11.84TSGIVTPSRR17 pKa = 11.84EE18 pKa = 4.07QASQNYY24 pKa = 9.17FSTNRR29 pKa = 11.84QKK31 pKa = 10.57MSLCYY36 pKa = 9.88GQPLKK41 pKa = 10.83LDD43 pKa = 3.5WPSQVLKK50 pKa = 8.64TTGKK54 pKa = 10.3SSFSRR59 pKa = 11.84KK60 pKa = 7.64TSVIFYY66 pKa = 10.61SSITMPP72 pKa = 3.95

Molecular weight: 8.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

1

6

2383

72

1646

397.2

44.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.973 ± 1.344

2.056 ± 0.422

4.784 ± 0.565

6.211 ± 0.837

4.07 ± 0.28

3.903 ± 0.346

3.063 ± 0.363

5.665 ± 0.803

6.798 ± 0.478

9.987 ± 0.792

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.93 ± 0.35

5.036 ± 0.866

5.204 ± 0.301

4.238 ± 0.33

4.7 ± 0.385

7.512 ± 1.275

5.623 ± 0.569

6.588 ± 0.406

1.259 ± 0.279

3.399 ± 0.599
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski