Gordonia phage LittleFella

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Terapinvirus; unclassified Terapinvirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LDN9|A0A7G8LDN9_9CAUD Uncharacterized protein OS=Gordonia phage LittleFella OX=2762411 GN=50 PE=4 SV=1
MM1 pKa = 7.68ASEE4 pKa = 5.75DD5 pKa = 3.7YY6 pKa = 10.94DD7 pKa = 3.98LQPEE11 pKa = 4.19RR12 pKa = 11.84CLVCGEE18 pKa = 4.66FIDD21 pKa = 4.05YY22 pKa = 10.82CLGHH26 pKa = 6.59GGGLEE31 pKa = 3.93LVVWDD36 pKa = 3.49QHH38 pKa = 6.69EE39 pKa = 5.13DD40 pKa = 3.79GDD42 pKa = 5.05HH43 pKa = 6.62SNCNPLACEE52 pKa = 4.13EE53 pKa = 4.14LRR55 pKa = 4.87

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LDJ4|A0A7G8LDJ4_9CAUD Uncharacterized protein OS=Gordonia phage LittleFella OX=2762411 GN=5 PE=4 SV=1
MM1 pKa = 7.62AFSQRR6 pKa = 11.84KK7 pKa = 8.44FKK9 pKa = 10.74IRR11 pKa = 11.84VQMRR15 pKa = 11.84DD16 pKa = 3.12KK17 pKa = 10.97SSKK20 pKa = 8.61KK21 pKa = 9.11PRR23 pKa = 11.84RR24 pKa = 11.84ITLVSQRR31 pKa = 11.84RR32 pKa = 11.84IVVNGKK38 pKa = 7.51VVKK41 pKa = 10.74AGAQADD47 pKa = 4.09LTKK50 pKa = 10.39PSQLRR55 pKa = 11.84NVVAKK60 pKa = 10.31AKK62 pKa = 9.81PRR64 pKa = 11.84RR65 pKa = 11.84NPAGGKK71 pKa = 9.41LKK73 pKa = 9.06QTASLTKK80 pKa = 10.45KK81 pKa = 9.55NLKK84 pKa = 9.43VGKK87 pKa = 9.78FF88 pKa = 3.36

Molecular weight: 9.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

20460

36

2231

222.4

24.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.404 ± 0.554

0.684 ± 0.09

6.403 ± 0.264

7.287 ± 0.307

3.309 ± 0.143

8.685 ± 0.367

1.852 ± 0.166

5.078 ± 0.229

5.645 ± 0.275

7.385 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.59 ± 0.114

3.905 ± 0.142

4.858 ± 0.21

4.189 ± 0.211

6.471 ± 0.283

5.543 ± 0.184

5.44 ± 0.197

6.593 ± 0.164

1.755 ± 0.154

2.923 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski