Pteropus associated gemycircularvirus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus ptero3

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTM5|A0A140CTM5_9VIRU RepA OS=Pteropus associated gemycircularvirus 3 OX=1985397 PE=3 SV=1
MM1 pKa = 7.45PQFLHH6 pKa = 6.4NYY8 pKa = 8.71RR9 pKa = 11.84YY10 pKa = 10.72VLVTYY15 pKa = 7.74PQSGNLDD22 pKa = 3.24PWRR25 pKa = 11.84VMEE28 pKa = 4.28RR29 pKa = 11.84FSYY32 pKa = 10.63LGAEE36 pKa = 4.39CIIGRR41 pKa = 11.84EE42 pKa = 3.99HH43 pKa = 7.87HH44 pKa = 6.83EE45 pKa = 4.6DD46 pKa = 3.53GGLHH50 pKa = 5.69LHH52 pKa = 6.24VFADD56 pKa = 4.7FGRR59 pKa = 11.84KK60 pKa = 8.16FRR62 pKa = 11.84SRR64 pKa = 11.84KK65 pKa = 7.76TNIFDD70 pKa = 3.43VDD72 pKa = 3.3GRR74 pKa = 11.84HH75 pKa = 6.67PNRR78 pKa = 11.84APSKK82 pKa = 7.79GTPEE86 pKa = 3.79KK87 pKa = 10.85GYY89 pKa = 10.88DD90 pKa = 3.47YY91 pKa = 10.69AIKK94 pKa = 10.83DD95 pKa = 3.47GDD97 pKa = 4.33VVCGGLGRR105 pKa = 11.84PEE107 pKa = 4.28PSSVGPGPSADD118 pKa = 2.51KK119 pKa = 9.89WARR122 pKa = 11.84ITQASDD128 pKa = 3.42RR129 pKa = 11.84DD130 pKa = 4.06EE131 pKa = 4.85FWDD134 pKa = 5.01LVHH137 pKa = 7.38QLDD140 pKa = 4.6PKK142 pKa = 10.89AAACSFNALSKK153 pKa = 9.33YY154 pKa = 10.43ADD156 pKa = 3.01WRR158 pKa = 11.84FAEE161 pKa = 4.12IAPEE165 pKa = 4.06YY166 pKa = 9.89EE167 pKa = 3.43HH168 pKa = 7.47DD169 pKa = 3.72GRR171 pKa = 11.84IKK173 pKa = 10.41FVPGDD178 pKa = 3.42ADD180 pKa = 5.31GRR182 pKa = 11.84DD183 pKa = 3.47DD184 pKa = 3.79WLSQSGIGLDD194 pKa = 3.77EE195 pKa = 4.83PFLGSRR201 pKa = 11.84VKK203 pKa = 10.91SLVLFGGTRR212 pKa = 11.84TGKK215 pKa = 5.99TTWARR220 pKa = 11.84SLGPHH225 pKa = 7.21IYY227 pKa = 10.58CIGLVSGTEE236 pKa = 4.11CAKK239 pKa = 10.12GTDD242 pKa = 2.88AKK244 pKa = 11.1YY245 pKa = 10.77AVFDD249 pKa = 5.04DD250 pKa = 3.32IRR252 pKa = 11.84GGFGFFHH259 pKa = 7.53GYY261 pKa = 9.92KK262 pKa = 9.15EE263 pKa = 3.99WLGAQPHH270 pKa = 5.31VSIKK274 pKa = 9.91QLYY277 pKa = 9.33RR278 pKa = 11.84EE279 pKa = 4.26PFYY282 pKa = 10.71MKK284 pKa = 9.68WGKK287 pKa = 7.38PTIWVCNTDD296 pKa = 3.58PRR298 pKa = 11.84LDD300 pKa = 3.79SYY302 pKa = 11.37PNNARR307 pKa = 11.84PDD309 pKa = 4.0FDD311 pKa = 3.38WMEE314 pKa = 4.29GNVDD318 pKa = 4.61FIEE321 pKa = 4.55VTSSLIEE328 pKa = 4.61SISHH332 pKa = 6.81ANTGG336 pKa = 3.26

Molecular weight: 37.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTM4|A0A140CTM4_9VIRU Replication-associated protein OS=Pteropus associated gemycircularvirus 3 OX=1985397 PE=3 SV=1
MM1 pKa = 8.16PYY3 pKa = 8.89RR4 pKa = 11.84TRR6 pKa = 11.84KK7 pKa = 7.19WGRR10 pKa = 11.84RR11 pKa = 11.84SYY13 pKa = 9.31TRR15 pKa = 11.84RR16 pKa = 11.84TTRR19 pKa = 11.84PGYY22 pKa = 9.51GGRR25 pKa = 11.84VSRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84YY31 pKa = 6.53PARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84YY38 pKa = 7.87PRR40 pKa = 11.84KK41 pKa = 7.79MTNKK45 pKa = 9.96RR46 pKa = 11.84ILNVTSHH53 pKa = 6.27KK54 pKa = 10.92KK55 pKa = 9.37KK56 pKa = 11.01DD57 pKa = 3.62NMCNYY62 pKa = 10.65SNTTPANPGGSTTYY76 pKa = 10.72SSNAAVLVGGQTYY89 pKa = 10.0IFPWIASARR98 pKa = 11.84PGGDD102 pKa = 2.84VSSNPGVGIEE112 pKa = 4.01MSNRR116 pKa = 11.84TSNVCYY122 pKa = 10.06MRR124 pKa = 11.84GLKK127 pKa = 10.1EE128 pKa = 4.18KK129 pKa = 10.41IQFQTSTGMAWQWRR143 pKa = 11.84RR144 pKa = 11.84ICFTAKK150 pKa = 10.32GNRR153 pKa = 11.84FYY155 pKa = 11.35QNDD158 pKa = 3.58SAGYY162 pKa = 8.13RR163 pKa = 11.84WSLLTSAGVVRR174 pKa = 11.84VVNNIAGTAQGSSFVDD190 pKa = 4.81AIFDD194 pKa = 3.88GQNGVDD200 pKa = 3.2WVSPFTARR208 pKa = 11.84VDD210 pKa = 3.46RR211 pKa = 11.84DD212 pKa = 3.5IFSVKK217 pKa = 9.85YY218 pKa = 10.39DD219 pKa = 3.31KK220 pKa = 8.97TTIIQSGNDD229 pKa = 3.15TGVMRR234 pKa = 11.84NYY236 pKa = 10.44NRR238 pKa = 11.84WHH240 pKa = 6.75AMNKK244 pKa = 9.77NLVFDD249 pKa = 5.18DD250 pKa = 4.91DD251 pKa = 4.34EE252 pKa = 6.8SGDD255 pKa = 3.82SEE257 pKa = 4.45IVGRR261 pKa = 11.84YY262 pKa = 9.29SNIGKK267 pKa = 9.41QGMGDD272 pKa = 3.79YY273 pKa = 10.77YY274 pKa = 11.38VIDD277 pKa = 4.89IIAAGTGSSSADD289 pKa = 3.11QASFQPEE296 pKa = 3.72ATLYY300 pKa = 8.73WHH302 pKa = 7.08EE303 pKa = 4.26KK304 pKa = 9.3

Molecular weight: 34.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

878

238

336

292.7

32.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.492 ± 0.065

1.708 ± 0.34

7.403 ± 0.746

3.986 ± 0.829

4.67 ± 0.582

10.706 ± 0.418

2.62 ± 0.674

4.67 ± 0.328

4.556 ± 0.282

4.784 ± 1.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.708 ± 0.523

4.1 ± 1.175

6.15 ± 1.242

2.62 ± 0.411

8.314 ± 0.807

7.745 ± 0.641

4.784 ± 1.239

5.809 ± 0.318

2.733 ± 0.108

4.442 ± 0.508
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski