Bacillus weihaiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3944 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3MQF2|A0A1L3MQF2_9BACI L D-transpeptidase OS=Bacillus weihaiensis OX=1547283 GN=A9C19_06825 PE=4 SV=1
MM1 pKa = 7.64AKK3 pKa = 8.9YY4 pKa = 9.07TIVDD8 pKa = 3.48KK9 pKa = 9.83DD10 pKa = 3.47TCIACGACGAAAPDD24 pKa = 3.33IYY26 pKa = 10.99DD27 pKa = 3.84YY28 pKa = 11.45DD29 pKa = 4.3DD30 pKa = 3.75EE31 pKa = 7.04GIAFVTLDD39 pKa = 3.64DD40 pKa = 3.93NQGVVEE46 pKa = 4.62VPDD49 pKa = 4.1ALEE52 pKa = 4.23EE53 pKa = 4.16DD54 pKa = 3.61MMDD57 pKa = 3.8AFEE60 pKa = 4.82GCPTDD65 pKa = 3.63SIKK68 pKa = 10.69ISDD71 pKa = 3.8EE72 pKa = 4.12KK73 pKa = 11.27FDD75 pKa = 3.84GDD77 pKa = 3.76ALKK80 pKa = 11.0FEE82 pKa = 4.68

Molecular weight: 8.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3MWV3|A0A1L3MWV3_9BACI Uncharacterized protein OS=Bacillus weihaiensis OX=1547283 GN=A9C19_20335 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.48VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.94VLSAA44 pKa = 4.11

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3944

0

3944

1122802

26

2415

284.7

32.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.331 ± 0.041

0.698 ± 0.01

5.067 ± 0.034

7.878 ± 0.047

4.616 ± 0.031

6.493 ± 0.037

2.182 ± 0.02

8.026 ± 0.046

7.152 ± 0.037

9.926 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.702 ± 0.019

4.438 ± 0.033

3.405 ± 0.023

3.807 ± 0.025

3.756 ± 0.027

6.311 ± 0.033

5.643 ± 0.028

7.005 ± 0.028

0.949 ± 0.015

3.614 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski