Escherichia phage megetsur

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Bonnellvirus; unclassified Bonnellvirus

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6C6XYV8|A0A6C6XYV8_9CAUD Putative Rz1A protein OS=Escherichia phage megetsur OX=2696417 GN=megetsur_51 PE=4 SV=1
MM1 pKa = 7.47ALVDD5 pKa = 3.57STAAEE10 pKa = 3.86RR11 pKa = 11.84AEE13 pKa = 5.0FVAACNMAAKK23 pKa = 9.82YY24 pKa = 10.73LNVWTEE30 pKa = 4.4DD31 pKa = 3.66YY32 pKa = 11.2FFDD35 pKa = 3.72TTNTEE40 pKa = 3.85LVVYY44 pKa = 8.54YY45 pKa = 9.81QEE47 pKa = 4.26SVTRR51 pKa = 11.84VQTALTNLTNAAAA64 pKa = 4.26

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6C6XYU0|A0A6C6XYU0_9CAUD Uncharacterized protein OS=Escherichia phage megetsur OX=2696417 GN=megetsur_33 PE=4 SV=1
MM1 pKa = 7.6SVLLWIVAVVWFALFSVVWLRR22 pKa = 11.84VTSTTRR28 pKa = 11.84HH29 pKa = 5.2TLTGLVQQDD38 pKa = 4.01LSCGYY43 pKa = 10.05SWLSSLLFCLPVLRR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84LYY61 pKa = 9.0EE62 pKa = 4.13SLSKK66 pKa = 10.79SHH68 pKa = 6.73

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13160

50

1034

243.7

26.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.395 ± 0.517

0.919 ± 0.146

6.018 ± 0.256

6.185 ± 0.318

3.412 ± 0.219

7.865 ± 0.345

1.869 ± 0.186

4.65 ± 0.226

4.369 ± 0.272

8.131 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.964 ± 0.275

4.271 ± 0.276

4.271 ± 0.347

4.985 ± 0.5

5.585 ± 0.268

5.79 ± 0.279

6.155 ± 0.369

7.188 ± 0.309

1.451 ± 0.132

3.526 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski