Frankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Frankiales; Frankiaceae; Frankia

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4441 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2JCB0|Q2JCB0_FRACC Uncharacterized protein OS=Frankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3) OX=106370 GN=Francci3_1706 PE=4 SV=1
MM1 pKa = 6.59TAARR5 pKa = 11.84TMRR8 pKa = 11.84VTISGVYY15 pKa = 9.6SEE17 pKa = 5.39YY18 pKa = 10.65EE19 pKa = 4.04VPANDD24 pKa = 4.7DD25 pKa = 3.18RR26 pKa = 11.84WNGFAVPGFTLDD38 pKa = 4.01QVRR41 pKa = 11.84QLAAEE46 pKa = 4.19TDD48 pKa = 3.62ALGATVDD55 pKa = 3.78ADD57 pKa = 4.43EE58 pKa = 5.61IDD60 pKa = 4.16TITIGDD66 pKa = 3.86DD67 pKa = 4.24GIVSVHH73 pKa = 5.98SGHH76 pKa = 6.7WNCTTIVVPDD86 pKa = 4.38PDD88 pKa = 3.13GLYY91 pKa = 10.6YY92 pKa = 10.8VGGYY96 pKa = 7.51EE97 pKa = 4.12WAWEE101 pKa = 3.99IVGNN105 pKa = 4.32

Molecular weight: 11.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2JFW3|Q2JFW3_FRACC Response regulator receiver protein OS=Frankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3) OX=106370 GN=Francci3_0443 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.72HH17 pKa = 5.74RR18 pKa = 11.84KK19 pKa = 7.77LLKK22 pKa = 8.07RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4441

0

4441

1512431

32

4606

340.6

36.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.681 ± 0.06

0.835 ± 0.011

6.053 ± 0.026

4.947 ± 0.035

2.619 ± 0.022

9.552 ± 0.041

2.275 ± 0.021

3.512 ± 0.024

1.473 ± 0.022

10.157 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.621 ± 0.012

1.638 ± 0.017

6.723 ± 0.044

2.532 ± 0.021

9.084 ± 0.036

5.27 ± 0.027

6.048 ± 0.03

8.694 ± 0.04

1.436 ± 0.015

1.85 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski