secondary endosymbiont of Heteropsylla cubana

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; aphid secondary symbionts

Average proteome isoelectric point is 7.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 576 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7GWM5|J7GWM5_9ENTR Acyl carrier protein OS=secondary endosymbiont of Heteropsylla cubana OX=134287 GN=acpP PE=3 SV=1
MM1 pKa = 7.89SITTPLLLQFSDD13 pKa = 3.82SAVKK17 pKa = 9.66QVKK20 pKa = 9.89ILIANEE26 pKa = 3.98DD27 pKa = 3.75NPNLKK32 pKa = 10.28LRR34 pKa = 11.84VYY36 pKa = 10.23IIGGGCSGFQYY47 pKa = 11.39AFTFDD52 pKa = 3.41DD53 pKa = 4.56HH54 pKa = 9.01VYY56 pKa = 10.43EE57 pKa = 5.68DD58 pKa = 4.46DD59 pKa = 3.69VTIEE63 pKa = 4.03KK64 pKa = 10.45EE65 pKa = 4.28GVILVVDD72 pKa = 4.33PMSLQYY78 pKa = 11.04LIGGSIDD85 pKa = 3.59YY86 pKa = 10.72VEE88 pKa = 4.5KK89 pKa = 11.26LEE91 pKa = 3.98GSRR94 pKa = 11.84FIVTNPNAKK103 pKa = 8.36NTCGCGASFSII114 pKa = 5.14

Molecular weight: 12.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J3YT87|J3YT87_9ENTR Uncharacterized protein OS=secondary endosymbiont of Heteropsylla cubana OX=134287 GN=A35E_00350 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.68RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 7.11GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.14NGRR28 pKa = 11.84QVLGRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.25SRR39 pKa = 11.84TRR41 pKa = 11.84LTVSCKK47 pKa = 10.3

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

576

0

576

182885

30

1407

317.5

35.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.43 ± 0.104

1.319 ± 0.035

4.728 ± 0.064

5.498 ± 0.096

4.114 ± 0.065

6.273 ± 0.088

2.52 ± 0.05

8.996 ± 0.092

6.622 ± 0.081

10.755 ± 0.12

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.445 ± 0.049

5.24 ± 0.083

3.648 ± 0.048

3.858 ± 0.06

5.477 ± 0.083

6.402 ± 0.07

5.239 ± 0.058

6.025 ± 0.102

1.092 ± 0.035

3.319 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski