Clostridium sp. (strain SY8519)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2601 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7V610|F7V610_CLOSS Predicted Rossmann fold nucleotide-binding protein involved in DNA uptake OS=Clostridium sp. (strain SY8519) OX=1042156 GN=Smf PE=3 SV=1
MM1 pKa = 7.92ADD3 pKa = 3.17HH4 pKa = 7.1FSNNTSDD11 pKa = 4.95PDD13 pKa = 3.54ILDD16 pKa = 4.01PEE18 pKa = 5.27DD19 pKa = 3.44IVVTIDD25 pKa = 4.26LEE27 pKa = 4.79DD28 pKa = 4.37GSSIDD33 pKa = 5.17CEE35 pKa = 3.82ILTIFSVGPQDD46 pKa = 4.92YY47 pKa = 10.43IALLPTDD54 pKa = 3.84EE55 pKa = 4.26QNQIVEE61 pKa = 4.46SKK63 pKa = 10.46GVYY66 pKa = 9.57LYY68 pKa = 10.54RR69 pKa = 11.84YY70 pKa = 10.01FEE72 pKa = 4.92DD73 pKa = 3.48EE74 pKa = 4.04QEE76 pKa = 4.24NYY78 pKa = 10.72SIGNIDD84 pKa = 3.4SDD86 pKa = 4.2EE87 pKa = 4.0EE88 pKa = 4.36LEE90 pKa = 4.1RR91 pKa = 11.84VIQAFHH97 pKa = 7.04RR98 pKa = 11.84LADD101 pKa = 3.84GEE103 pKa = 4.58GG104 pKa = 3.2

Molecular weight: 11.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7V2K2|F7V2K2_CLOSS Uncharacterized protein OS=Clostridium sp. (strain SY8519) OX=1042156 GN=CXIVA_10140 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.56QPKK8 pKa = 9.32KK9 pKa = 7.54RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.77VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 8.85KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2601

0

2601

830445

39

2289

319.3

35.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.512 ± 0.053

1.549 ± 0.023

5.61 ± 0.038

7.237 ± 0.059

3.797 ± 0.034

7.458 ± 0.044

1.996 ± 0.021

6.797 ± 0.046

6.036 ± 0.052

8.735 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.962 ± 0.024

3.747 ± 0.031

3.683 ± 0.031

3.431 ± 0.025

5.594 ± 0.048

5.879 ± 0.048

5.563 ± 0.049

6.656 ± 0.041

0.888 ± 0.015

3.871 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski