Flavobacterium pallidum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2931 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1SHV9|A0A2S1SHV9_9FLAO Cyanophycin synthase OS=Flavobacterium pallidum OX=2172098 GN=cphA PE=3 SV=1
MM1 pKa = 7.59KK2 pKa = 10.51KK3 pKa = 10.23NLLFLIALICFSLGNAQSISIVGEE27 pKa = 4.0AVGGWEE33 pKa = 4.95DD34 pKa = 4.23NDD36 pKa = 4.09PNTPDD41 pKa = 3.19THH43 pKa = 8.76IMSTVDD49 pKa = 3.31NINYY53 pKa = 7.51TFSNLAVATADD64 pKa = 3.89PNGGGAKK71 pKa = 9.79FRR73 pKa = 11.84QDD75 pKa = 3.37GAWAINWGNAAFPSGTGTQNGANILTVAGTYY106 pKa = 10.55DD107 pKa = 3.26VTFNRR112 pKa = 11.84LTGAYY117 pKa = 9.96SFTNVVTYY125 pKa = 8.94PSIGMIGTAVNANGFNGPDD144 pKa = 3.05VDD146 pKa = 4.19MVTFDD151 pKa = 4.49GITYY155 pKa = 8.38TITNYY160 pKa = 10.43NFLSGEE166 pKa = 4.1MKK168 pKa = 10.44FRR170 pKa = 11.84LDD172 pKa = 4.85DD173 pKa = 3.45SWDD176 pKa = 3.72NSWGSTAFPSGTAVLNVPDD195 pKa = 5.49NIPVPAGTYY204 pKa = 8.9SVSFNRR210 pKa = 11.84ITGDD214 pKa = 3.32YY215 pKa = 10.37SFTFPSIGIIGTAVSAGGFNDD236 pKa = 3.97PDD238 pKa = 4.26TDD240 pKa = 4.4LMTADD245 pKa = 3.34GVHH248 pKa = 5.25YY249 pKa = 10.55VLYY252 pKa = 10.74NHH254 pKa = 6.21VFTDD258 pKa = 4.12GLAKK262 pKa = 10.41FRR264 pKa = 11.84QGDD267 pKa = 3.3NWDD270 pKa = 4.16VNWGNADD277 pKa = 3.25GDD279 pKa = 4.19FPSGIGTQGGTDD291 pKa = 3.18IPVTAGTYY299 pKa = 7.21TVNFNRR305 pKa = 11.84EE306 pKa = 3.37TGAYY310 pKa = 9.95SFDD313 pKa = 3.78APVVFANIGVIGTAVSAGGFDD334 pKa = 5.84DD335 pKa = 5.91PDD337 pKa = 3.58TDD339 pKa = 3.39MTTTDD344 pKa = 4.33GITYY348 pKa = 8.93TLEE351 pKa = 3.95NYY353 pKa = 9.5VFANGQLKK361 pKa = 9.77FRR363 pKa = 11.84QDD365 pKa = 4.12DD366 pKa = 3.56VWDD369 pKa = 4.13NNWGGVGFPGDD380 pKa = 3.37TANFNGPNIPVTAGTYY396 pKa = 9.2TLTFNINTLAYY407 pKa = 9.84SFTGTPLYY415 pKa = 8.21PTVGILGTAVSANAFNGNDD434 pKa = 3.37INLTTTDD441 pKa = 3.41GEE443 pKa = 4.73TYY445 pKa = 10.46ILEE448 pKa = 4.45NYY450 pKa = 8.45TFVAGEE456 pKa = 3.86AKK458 pKa = 9.94FRR460 pKa = 11.84QDD462 pKa = 4.79DD463 pKa = 3.37AWDD466 pKa = 3.9INWGNPAFPTGIATQGGSNITVPAGTFNVFFTRR499 pKa = 11.84STGAYY504 pKa = 8.5EE505 pKa = 4.11FTTVGAFPAVGLIGTAVDD523 pKa = 3.74ANGFDD528 pKa = 5.12GPDD531 pKa = 3.03TDD533 pKa = 3.63MATTNGILYY542 pKa = 8.91TLDD545 pKa = 3.41GVTLMDD551 pKa = 4.16GEE553 pKa = 4.53AKK555 pKa = 10.05FRR557 pKa = 11.84QDD559 pKa = 4.85DD560 pKa = 3.68AWDD563 pKa = 3.84MSWGSVNFPSGTGTVGGANIMVTAGNYY590 pKa = 9.57DD591 pKa = 3.38VTFNRR596 pKa = 11.84LTGDD600 pKa = 3.45YY601 pKa = 10.47SFDD604 pKa = 3.33STLPVYY610 pKa = 10.18PSIGIIGDD618 pKa = 3.53AVTVDD623 pKa = 4.06GFTGADD629 pKa = 2.85VDD631 pKa = 3.94MQTTDD636 pKa = 3.69GITYY640 pKa = 9.28TLMAYY645 pKa = 8.41TFTDD649 pKa = 4.23GEE651 pKa = 4.36AKK653 pKa = 10.01FRR655 pKa = 11.84QDD657 pKa = 5.42DD658 pKa = 3.61NWDD661 pKa = 3.93VNWGSVDD668 pKa = 4.09FPSGTGTQGGSNIEE682 pKa = 4.31VAAGTYY688 pKa = 10.29NVTFNILTGAYY699 pKa = 10.17SFDD702 pKa = 3.53FVNIGILGTALNGFDD717 pKa = 5.56VEE719 pKa = 4.48DD720 pKa = 3.93TDD722 pKa = 3.65MTTTDD727 pKa = 3.19GVNYY731 pKa = 9.87YY732 pKa = 10.74LDD734 pKa = 5.35GIHH737 pKa = 6.41LTDD740 pKa = 3.6GLIKK744 pKa = 10.54FRR746 pKa = 11.84QNDD749 pKa = 3.14NWTVNWGGTDD759 pKa = 3.94FPGGTYY765 pKa = 9.43TLSGEE770 pKa = 4.45NIPVTEE776 pKa = 4.17GDD778 pKa = 3.85YY779 pKa = 10.72IITFNRR785 pKa = 11.84LTGVGQFDD793 pKa = 4.05EE794 pKa = 5.17VILGVNEE801 pKa = 3.79PAQVTFSVFPNPSSEE816 pKa = 3.79SWNFSSRR823 pKa = 11.84GDD825 pKa = 3.62NLHH828 pKa = 7.26DD829 pKa = 4.42IEE831 pKa = 7.03IIDD834 pKa = 3.81ATGKK838 pKa = 8.29TVVSKK843 pKa = 10.92NLSGNHH849 pKa = 4.75ATIDD853 pKa = 3.63ASSLSSGLYY862 pKa = 9.01FAKK865 pKa = 9.52LTSDD869 pKa = 3.92KK870 pKa = 10.85GSKK873 pKa = 8.15TIKK876 pKa = 10.07VIKK879 pKa = 10.1NN880 pKa = 3.24

Molecular weight: 93.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1SLR8|A0A2S1SLR8_9FLAO Uncharacterized protein OS=Flavobacterium pallidum OX=2172098 GN=HYN49_12435 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.3RR3 pKa = 11.84RR4 pKa = 11.84IKK6 pKa = 10.44RR7 pKa = 11.84SRR9 pKa = 11.84KK10 pKa = 7.71NSKK13 pKa = 9.39RR14 pKa = 11.84KK15 pKa = 9.44NRR17 pKa = 11.84LGVKK21 pKa = 10.2NSLVNNINARR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 9.34GVARR38 pKa = 11.84SKK40 pKa = 10.47RR41 pKa = 11.84KK42 pKa = 9.38SHH44 pKa = 6.56ISKK47 pKa = 10.22KK48 pKa = 9.93AYY50 pKa = 9.61KK51 pKa = 10.42AMRR54 pKa = 11.84EE55 pKa = 3.93NWKK58 pKa = 10.31KK59 pKa = 10.89

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2931

0

2931

1068508

22

5887

364.6

40.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.417 ± 0.063

0.846 ± 0.02

5.748 ± 0.06

5.75 ± 0.073

5.165 ± 0.045

6.876 ± 0.062

1.757 ± 0.026

7.462 ± 0.05

6.86 ± 0.099

8.596 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.331 ± 0.03

6.133 ± 0.073

3.749 ± 0.047

3.343 ± 0.028

3.395 ± 0.038

6.593 ± 0.056

6.575 ± 0.139

6.38 ± 0.041

1.013 ± 0.017

4.012 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski