Flavobacterium macacae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3009 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P3WJN9|A0A3P3WJN9_9FLAO XRE family transcriptional regulator OS=Flavobacterium macacae OX=2488993 GN=EG849_05450 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.36KK3 pKa = 10.47VILSVAFMLATGVAFAQEE21 pKa = 3.79NSATVSQEE29 pKa = 3.51GTSNGSSVVQVGRR42 pKa = 11.84EE43 pKa = 3.71NTATVNQQGNVAPGVASGLEE63 pKa = 3.83NSSIVDD69 pKa = 3.41QLGDD73 pKa = 3.46EE74 pKa = 4.33NMADD78 pKa = 3.18VDD80 pKa = 3.91QRR82 pKa = 11.84GDD84 pKa = 3.64FNISDD89 pKa = 3.89VNQDD93 pKa = 2.92GDD95 pKa = 3.95NNEE98 pKa = 4.05ATVSQGNGGGQAEE111 pKa = 4.35NNKK114 pKa = 10.27AYY116 pKa = 8.44ATQLGDD122 pKa = 3.9GNKK125 pKa = 8.66STQTQRR131 pKa = 11.84FDD133 pKa = 3.75NNTGTVTQTGDD144 pKa = 3.83DD145 pKa = 3.57NVATQFQTSGPDD157 pKa = 3.38GSAGNTATATQHH169 pKa = 6.5GDD171 pKa = 3.14GNEE174 pKa = 3.6VDD176 pKa = 3.97QRR178 pKa = 11.84QEE180 pKa = 3.86GHH182 pKa = 6.53SNTALATQGINPSSFQALNNYY203 pKa = 9.02ALQDD207 pKa = 3.45QDD209 pKa = 3.91GDD211 pKa = 4.36SNNAMISQSNQDD223 pKa = 3.34NEE225 pKa = 4.23AYY227 pKa = 9.95QYY229 pKa = 11.11QIGDD233 pKa = 4.06SNSATVWQDD242 pKa = 3.05EE243 pKa = 4.43VQPTINGGDD252 pKa = 3.76LAVQYY257 pKa = 10.82QEE259 pKa = 4.58GDD261 pKa = 3.76SNAATIDD268 pKa = 3.14QGEE271 pKa = 4.45NYY273 pKa = 10.47LGAPYY278 pKa = 10.14NAYY281 pKa = 9.26PHH283 pKa = 7.12GDD285 pKa = 3.4NEE287 pKa = 4.71SYY289 pKa = 10.45QVQLGDD295 pKa = 4.93DD296 pKa = 3.97NIAYY300 pKa = 9.74SKK302 pKa = 11.01QSGTSNISGQLQMGEE317 pKa = 4.43GNNANHH323 pKa = 5.62VQVGNSNVAATYY335 pKa = 10.83QNGEE339 pKa = 4.65DD340 pKa = 3.62NTSSIDD346 pKa = 3.81QLGLGNGALTIQMGDD361 pKa = 2.98GHH363 pKa = 6.75MSTVTQVGNTNVSFVSQSNN382 pKa = 3.16

Molecular weight: 39.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P3W5L1|A0A3P3W5L1_9FLAO TonB-dependent receptor OS=Flavobacterium macacae OX=2488993 GN=EG849_10320 PE=4 SV=1
MM1 pKa = 7.47RR2 pKa = 11.84RR3 pKa = 11.84IAFAISAVGLAVSLYY18 pKa = 10.17ALPGRR23 pKa = 11.84FADD26 pKa = 3.84NSADD30 pKa = 3.44LTSYY34 pKa = 9.97ILKK37 pKa = 10.55GSFVLMFLVSLIINVICIHH56 pKa = 7.26DD57 pKa = 4.09YY58 pKa = 10.31FRR60 pKa = 11.84KK61 pKa = 9.79SKK63 pKa = 11.17NNIRR67 pKa = 11.84TIRR70 pKa = 11.84QYY72 pKa = 11.14YY73 pKa = 7.52LQRR76 pKa = 11.84SIRR79 pKa = 3.6

Molecular weight: 9.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3009

0

3009

977937

33

2412

325.0

36.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.591 ± 0.046

0.739 ± 0.014

5.378 ± 0.029

6.78 ± 0.055

5.486 ± 0.039

6.334 ± 0.046

1.688 ± 0.022

7.942 ± 0.046

8.02 ± 0.057

9.103 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.254 ± 0.021

6.141 ± 0.051

3.388 ± 0.024

3.4 ± 0.022

3.302 ± 0.032

6.607 ± 0.036

5.76 ± 0.062

6.187 ± 0.035

0.963 ± 0.016

3.937 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski