Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Betaherpesvirinae; Cytomegalovirus; Human betaherpesvirus 5

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 180 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|F5H8R6|UL96_HCMVM Protein UL96 OS=Human cytomegalovirus (strain Merlin) OX=295027 GN=UL96 PE=3 SV=1
MM1 pKa = 7.29SLFYY5 pKa = 10.51RR6 pKa = 11.84AVALGTLSALVWYY19 pKa = 7.5STSILAEE26 pKa = 4.05INEE29 pKa = 4.36NSCSSSSVDD38 pKa = 4.59HH39 pKa = 7.01EE40 pKa = 4.54DD41 pKa = 3.96CEE43 pKa = 4.58EE44 pKa = 4.11PDD46 pKa = 3.55EE47 pKa = 4.67IVRR50 pKa = 11.84EE51 pKa = 4.12EE52 pKa = 3.71QDD54 pKa = 3.41YY55 pKa = 10.84RR56 pKa = 11.84ALLAFSLVICGTLLVTCVII75 pKa = 4.52

Molecular weight: 8.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|F5HBC6|PP71_HCMVM Protein pp71 OS=Human cytomegalovirus (strain Merlin) OX=295027 GN=UL82 PE=3 SV=1
MM1 pKa = 7.61GNPRR5 pKa = 11.84SPLDD9 pKa = 3.11RR10 pKa = 11.84RR11 pKa = 11.84LRR13 pKa = 11.84FRR15 pKa = 11.84NVPFLPPRR23 pKa = 11.84IAASSTTTARR33 pKa = 11.84SLKK36 pKa = 10.05AKK38 pKa = 8.48TMEE41 pKa = 3.93MRR43 pKa = 11.84FTIAWMWFPSVLLILGLLTPPSNGCTVDD71 pKa = 2.93VGRR74 pKa = 11.84NVSIRR79 pKa = 11.84EE80 pKa = 3.74QCRR83 pKa = 11.84LRR85 pKa = 11.84NGATFSKK92 pKa = 10.98GDD94 pKa = 3.37IEE96 pKa = 5.57GNFSGPVVVEE106 pKa = 4.44LDD108 pKa = 3.82YY109 pKa = 11.77EE110 pKa = 4.91DD111 pKa = 4.89IDD113 pKa = 3.73ITGEE117 pKa = 3.9RR118 pKa = 11.84QRR120 pKa = 11.84LRR122 pKa = 11.84FHH124 pKa = 7.13LSGLGCPTKK133 pKa = 10.92EE134 pKa = 4.84NIRR137 pKa = 11.84KK138 pKa = 10.05DD139 pKa = 3.53NEE141 pKa = 4.06SDD143 pKa = 3.66VNGGIRR149 pKa = 11.84WALYY153 pKa = 9.78IQTGDD158 pKa = 2.97AKK160 pKa = 10.84YY161 pKa = 10.53GIRR164 pKa = 11.84NQHH167 pKa = 5.28LSIRR171 pKa = 11.84LMYY174 pKa = 10.14PGEE177 pKa = 4.51KK178 pKa = 8.85NTQQLLGSDD187 pKa = 4.87FSCEE191 pKa = 3.4RR192 pKa = 11.84HH193 pKa = 5.67RR194 pKa = 11.84RR195 pKa = 11.84PSTPLGKK202 pKa = 9.93NAEE205 pKa = 4.32VPPATRR211 pKa = 11.84TSSTYY216 pKa = 10.31GVLSAFVVWIGSGLNIIWWTGIVLLAADD244 pKa = 4.13ALGLGEE250 pKa = 4.1RR251 pKa = 11.84WLRR254 pKa = 11.84LALSHH259 pKa = 6.94RR260 pKa = 11.84DD261 pKa = 2.94KK262 pKa = 11.15HH263 pKa = 5.82HH264 pKa = 7.0ASRR267 pKa = 11.84TAALQCQRR275 pKa = 11.84DD276 pKa = 3.56MLLRR280 pKa = 11.84QRR282 pKa = 11.84RR283 pKa = 11.84RR284 pKa = 11.84ARR286 pKa = 11.84RR287 pKa = 11.84LHH289 pKa = 5.93AVSEE293 pKa = 4.5GKK295 pKa = 9.93LQEE298 pKa = 4.03EE299 pKa = 5.07KK300 pKa = 10.52KK301 pKa = 10.37RR302 pKa = 11.84QSALVWNVEE311 pKa = 3.71ARR313 pKa = 11.84PFPSTHH319 pKa = 5.48QLIVLPPPVASAPPAVPSQPPEE341 pKa = 3.92YY342 pKa = 10.41SSVFPPVV349 pKa = 2.78

Molecular weight: 39.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

168

12

180

66935

47

2241

371.9

41.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.982 ± 0.202

2.461 ± 0.107

4.968 ± 0.127

5.396 ± 0.146

3.727 ± 0.111

6.233 ± 0.221

3.172 ± 0.092

3.435 ± 0.129

2.903 ± 0.098

10.352 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.056 ± 0.071

3.266 ± 0.131

5.922 ± 0.204

3.665 ± 0.2

7.74 ± 0.187

7.772 ± 0.23

6.784 ± 0.221

7.498 ± 0.155

1.43 ± 0.083

3.239 ± 0.108
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski