Synechococcus sp. (strain JA-2-3B a(2-13)) (Cyanobacteria bacterium Yellowstone B-Prime)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2835 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2JPF3|Q2JPF3_SYNJB Putative membrane protein OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=CYB_0341 PE=4 SV=1
MM1 pKa = 7.16ALEE4 pKa = 4.43YY5 pKa = 9.68PSHH8 pKa = 6.26LRR10 pKa = 11.84YY11 pKa = 10.07VDD13 pKa = 3.06THH15 pKa = 6.2EE16 pKa = 4.76YY17 pKa = 9.84IHH19 pKa = 7.97VEE21 pKa = 3.78DD22 pKa = 6.07DD23 pKa = 3.14IAVIGITAYY32 pKa = 10.39AVDD35 pKa = 3.84QLGDD39 pKa = 3.45IVFVGLPEE47 pKa = 4.37EE48 pKa = 4.27GTEE51 pKa = 4.02IEE53 pKa = 4.33KK54 pKa = 11.17GEE56 pKa = 4.26SFGSVEE62 pKa = 4.1SVKK65 pKa = 10.74AVEE68 pKa = 4.92DD69 pKa = 4.29LYY71 pKa = 11.54APLSGEE77 pKa = 4.07VVAVNTAVVEE87 pKa = 4.58SPEE90 pKa = 4.12SLADD94 pKa = 3.83DD95 pKa = 4.75PYY97 pKa = 11.67GDD99 pKa = 3.29GWLIKK104 pKa = 10.6VRR106 pKa = 11.84IANPEE111 pKa = 4.02DD112 pKa = 4.77LEE114 pKa = 4.36DD115 pKa = 3.76TMSAEE120 pKa = 4.47AYY122 pKa = 9.67ASLVEE127 pKa = 4.46GSS129 pKa = 3.4

Molecular weight: 13.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2JHZ0|Q2JHZ0_SYNJB Membrane protein putative OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=CYB_2861 PE=4 SV=1
MM1 pKa = 6.59TQRR4 pKa = 11.84TLRR7 pKa = 11.84GTNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84IRR15 pKa = 11.84VSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TATGRR29 pKa = 11.84QVLRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.95GRR40 pKa = 11.84YY41 pKa = 8.41RR42 pKa = 11.84LAVSS46 pKa = 3.85

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2835

0

2835

860160

30

2505

303.4

33.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.817 ± 0.045

1.085 ± 0.019

4.37 ± 0.028

6.276 ± 0.045

3.449 ± 0.033

7.766 ± 0.045

2.019 ± 0.023

4.808 ± 0.035

2.907 ± 0.035

12.388 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.736 ± 0.021

2.533 ± 0.027

6.205 ± 0.047

5.785 ± 0.052

7.169 ± 0.045

5.732 ± 0.032

4.65 ± 0.03

6.918 ± 0.039

1.79 ± 0.027

2.595 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski