Imtechella halotolerans K1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Imtechella; Imtechella halotolerans

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2694 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0WBR2|I0WBR2_9FLAO RNA-directed DNA polymerase OS=Imtechella halotolerans K1 OX=946077 GN=W5A_09695 PE=4 SV=1
TT1 pKa = 5.45TTFSATYY8 pKa = 9.21TITQSDD14 pKa = 3.26IDD16 pKa = 3.87AGVVVNQATAEE27 pKa = 4.21GSDD30 pKa = 3.69PEE32 pKa = 4.56GNPVIDD38 pKa = 5.3FSGTDD43 pKa = 3.13IDD45 pKa = 4.36NDD47 pKa = 3.67EE48 pKa = 4.15PTITVIEE55 pKa = 4.27STIPLAEE62 pKa = 5.38DD63 pKa = 3.87DD64 pKa = 4.58SVTTGMKK71 pKa = 9.68QPVIISILDD80 pKa = 3.29NDD82 pKa = 3.91EE83 pKa = 4.76ANDD86 pKa = 3.44VGFDD90 pKa = 3.56YY91 pKa = 11.28SSIEE95 pKa = 4.05IISYY99 pKa = 8.88PIHH102 pKa = 5.29GTITIMPDD110 pKa = 2.79GTVLYY115 pKa = 10.05EE116 pKa = 3.64PRR118 pKa = 11.84LDD120 pKa = 3.6VKK122 pKa = 11.37YY123 pKa = 10.76SGTDD127 pKa = 2.7AFTYY131 pKa = 10.17RR132 pKa = 11.84VQDD135 pKa = 4.37GNGKK139 pKa = 5.65WTNVATVTITIPGLFIPNVITPNGDD164 pKa = 3.34TKK166 pKa = 11.51NDD168 pKa = 3.28TFEE171 pKa = 5.86IIGLQSYY178 pKa = 8.4TSAEE182 pKa = 3.81VLIYY186 pKa = 10.52NRR188 pKa = 11.84WGNEE192 pKa = 3.41VYY194 pKa = 10.63RR195 pKa = 11.84NSKK198 pKa = 9.64YY199 pKa = 11.12DD200 pKa = 3.22NTFNGEE206 pKa = 4.0GLNEE210 pKa = 3.7GTYY213 pKa = 10.45YY214 pKa = 11.27YY215 pKa = 10.62LVKK218 pKa = 9.8LTNVNGGVEE227 pKa = 4.89TYY229 pKa = 10.52QGWIFIKK236 pKa = 10.52RR237 pKa = 3.45

Molecular weight: 26.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0WGG3|I0WGG3_9FLAO Sugar phosphate nucleotidyl transferase OS=Imtechella halotolerans K1 OX=946077 GN=W5A_04673 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.19RR22 pKa = 11.84MATANGRR29 pKa = 11.84KK30 pKa = 8.93VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.52LSVSSEE48 pKa = 3.87PRR50 pKa = 11.84HH51 pKa = 5.92KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2694

0

2694

933156

25

3778

346.4

39.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.368 ± 0.04

0.694 ± 0.016

5.199 ± 0.032

6.579 ± 0.038

5.09 ± 0.033

6.453 ± 0.045

1.968 ± 0.021

7.861 ± 0.041

7.127 ± 0.053

9.462 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.377 ± 0.022

5.757 ± 0.047

3.524 ± 0.027

3.696 ± 0.024

3.704 ± 0.032

6.665 ± 0.035

5.811 ± 0.044

6.342 ± 0.039

1.1 ± 0.017

4.224 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski