Firmicutes bacterium CAG:272

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2248 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6UCN4|R6UCN4_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:272 OX=1263015 GN=BN580_00525 PE=4 SV=1
MM1 pKa = 8.16DD2 pKa = 3.88KK3 pKa = 10.43TVFDD7 pKa = 4.05SKK9 pKa = 11.1VRR11 pKa = 11.84ALSRR15 pKa = 11.84DD16 pKa = 3.6DD17 pKa = 5.05YY18 pKa = 10.86YY19 pKa = 11.4QSPDD23 pKa = 3.52FQCAQTGGYY32 pKa = 7.36PTMLCINWDD41 pKa = 3.75EE42 pKa = 4.1EE43 pKa = 4.54KK44 pKa = 10.88AWLKK48 pKa = 10.61PNEE51 pKa = 4.15FVDD54 pKa = 3.71PCGADD59 pKa = 3.0VAEE62 pKa = 4.15YY63 pKa = 10.34EE64 pKa = 4.6KK65 pKa = 11.21LCADD69 pKa = 4.28FGIRR73 pKa = 11.84LCSDD77 pKa = 2.98IEE79 pKa = 4.32DD80 pKa = 3.95FNGLLKK86 pKa = 10.66EE87 pKa = 4.48LGPDD91 pKa = 3.09AVEE94 pKa = 3.97NATLYY99 pKa = 10.67EE100 pKa = 4.95DD101 pKa = 4.39EE102 pKa = 6.19DD103 pKa = 4.78FDD105 pKa = 5.79LSS107 pKa = 3.73

Molecular weight: 12.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6U959|R6U959_9FIRM DNA polymerase I OS=Firmicutes bacterium CAG:272 OX=1263015 GN=polA PE=3 SV=1
MM1 pKa = 7.51RR2 pKa = 11.84VIEE5 pKa = 4.46FGRR8 pKa = 11.84IPRR11 pKa = 11.84AVGLTVRR18 pKa = 11.84QIAVIGKK25 pKa = 8.68IALNTEE31 pKa = 4.14IEE33 pKa = 4.31ALTVDD38 pKa = 3.55KK39 pKa = 10.72QIQVVFVILNVNVGSQLGRR58 pKa = 11.84TVWPGRR64 pKa = 11.84NIVLPDD70 pKa = 3.37IEE72 pKa = 4.81TEE74 pKa = 4.17TVLYY78 pKa = 10.38LFNRR82 pKa = 11.84SVLGIRR88 pKa = 11.84RR89 pKa = 11.84LVCLGGIDD97 pKa = 4.13IKK99 pKa = 11.51DD100 pKa = 3.53DD101 pKa = 3.35VLRR104 pKa = 11.84ILDD107 pKa = 3.64CQRR110 pKa = 11.84PYY112 pKa = 10.69RR113 pKa = 11.84VAVVFFVRR121 pKa = 11.84QILGCVLFPALCLTAAVGRR140 pKa = 11.84AGFISAGSARR150 pKa = 11.84RR151 pKa = 11.84KK152 pKa = 9.59RR153 pKa = 3.58

Molecular weight: 16.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2248

0

2248

665660

29

2791

296.1

32.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.445 ± 0.06

1.767 ± 0.024

6.257 ± 0.043

6.611 ± 0.058

4.237 ± 0.032

7.938 ± 0.054

1.612 ± 0.021

6.651 ± 0.044

5.857 ± 0.047

8.401 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.63 ± 0.024

3.964 ± 0.037

3.856 ± 0.029

2.016 ± 0.031

5.389 ± 0.047

6.518 ± 0.043

5.77 ± 0.047

7.132 ± 0.044

0.864 ± 0.018

4.079 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski