Sphaerochaeta coccoides (strain ATCC BAA-1237 / DSM 17374 / SPN1) (Spirochaeta coccoides)

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Sphaerochaetaceae; Sphaerochaeta; Sphaerochaeta coccoides

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1819 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4GM11|F4GM11_SPHCD Uncharacterized protein OS=Sphaerochaeta coccoides (strain ATCC BAA-1237 / DSM 17374 / SPN1) OX=760011 GN=Spico_1278 PE=4 SV=1
MM1 pKa = 7.72KK2 pKa = 10.32KK3 pKa = 10.09RR4 pKa = 11.84LFVLMVFLGVSCVVLFAGPFGIDD27 pKa = 2.71MKK29 pKa = 10.82MSLEE33 pKa = 4.09DD34 pKa = 3.76LEE36 pKa = 4.54NAGFFPEE43 pKa = 3.78VDD45 pKa = 4.24EE46 pKa = 4.83IRR48 pKa = 11.84TVYY51 pKa = 8.52TWYY54 pKa = 10.84SVFPSKK60 pKa = 10.57PHH62 pKa = 7.12DD63 pKa = 3.78LFEE66 pKa = 4.54QYY68 pKa = 9.92FVAVDD73 pKa = 3.84DD74 pKa = 4.85EE75 pKa = 4.87YY76 pKa = 11.77GVCKK80 pKa = 10.31IVAVGKK86 pKa = 10.13DD87 pKa = 3.54IEE89 pKa = 4.42TSTYY93 pKa = 9.62GEE95 pKa = 4.01NVIDD99 pKa = 3.67AFEE102 pKa = 4.02RR103 pKa = 11.84VEE105 pKa = 5.15KK106 pKa = 10.37GVSWTYY112 pKa = 11.48GEE114 pKa = 4.02VDD116 pKa = 3.66VVFDD120 pKa = 3.8YY121 pKa = 11.59LDD123 pKa = 3.97YY124 pKa = 11.04EE125 pKa = 4.87SVWNEE130 pKa = 3.82PEE132 pKa = 3.38DD133 pKa = 3.53WMYY136 pKa = 11.49GLYY139 pKa = 10.27YY140 pKa = 10.08EE141 pKa = 4.92EE142 pKa = 4.1RR143 pKa = 11.84TLLAFWEE150 pKa = 4.4PTLDD154 pKa = 5.12DD155 pKa = 4.22IDD157 pKa = 6.36NIMLEE162 pKa = 4.27ANALSSTVGWLMLGYY177 pKa = 10.46EE178 pKa = 4.27SLHH181 pKa = 6.1WSDD184 pKa = 4.53VLDD187 pKa = 4.42RR188 pKa = 11.84DD189 pKa = 3.73

Molecular weight: 21.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4GLS9|F4GLS9_SPHCD Uncharacterized protein OS=Sphaerochaeta coccoides (strain ATCC BAA-1237 / DSM 17374 / SPN1) OX=760011 GN=Spico_1264 PE=4 SV=1
MM1 pKa = 7.56NKK3 pKa = 9.57TIQTTLKK10 pKa = 10.35RR11 pKa = 11.84MSGRR15 pKa = 11.84NEE17 pKa = 3.72PYY19 pKa = 10.09IFLILILLSILIQVRR34 pKa = 11.84SGQFFSSNNLVDD46 pKa = 3.29IASALIVPGLFAVATFMVIVSGGIDD71 pKa = 3.28VSFPALASLSVYY83 pKa = 10.59AITKK87 pKa = 7.87MFLDD91 pKa = 3.88IGYY94 pKa = 9.33QGNVLLPLACAVFLGALLGAFNGLFVGYY122 pKa = 9.79FGLPAMIVTLGSSSVFRR139 pKa = 11.84GIMQGALNSRR149 pKa = 11.84QLTMIPEE156 pKa = 4.41PMRR159 pKa = 11.84IFGTTPLFIARR170 pKa = 11.84NPVSGLTSRR179 pKa = 11.84LPLPFMILVVVAVLVFLMMRR199 pKa = 11.84FTMFGRR205 pKa = 11.84GIYY208 pKa = 10.22AIGGSEE214 pKa = 4.17VSAHH218 pKa = 6.08RR219 pKa = 11.84AGFHH223 pKa = 5.13VKK225 pKa = 8.36RR226 pKa = 11.84TKK228 pKa = 10.39FLLYY232 pKa = 10.25VFAGIIASLAGFARR246 pKa = 11.84VTMMQQAHH254 pKa = 5.98PTNMLGMEE262 pKa = 4.23MNIIAGVVLGGTAITGGRR280 pKa = 11.84GTLLGCFLGTLLIVIVEE297 pKa = 4.1NSLILIGVPTSWKK310 pKa = 10.47SVFTGLLIIIGTGVSAYY327 pKa = 10.71QGMLMGKK334 pKa = 9.45KK335 pKa = 9.84RR336 pKa = 11.84GIGTKK341 pKa = 7.07EE342 pKa = 3.65VRR344 pKa = 11.84SS345 pKa = 3.82

Molecular weight: 36.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1819

0

1819

660275

34

2867

363.0

40.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.477 ± 0.052

1.022 ± 0.019

5.842 ± 0.044

5.91 ± 0.06

4.335 ± 0.045

7.859 ± 0.059

2.101 ± 0.023

6.672 ± 0.049

4.969 ± 0.049

9.41 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.909 ± 0.027

3.573 ± 0.038

4.101 ± 0.035

2.939 ± 0.032

5.379 ± 0.046

6.864 ± 0.049

5.935 ± 0.057

7.291 ± 0.045

1.195 ± 0.021

3.216 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski