Clostridium sp. CAG:590

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2354 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5ECM4|R5ECM4_9CLOT AHS2 domain-containing protein OS=Clostridium sp. CAG:590 OX=1262825 GN=BN724_01092 PE=4 SV=1
MM1 pKa = 7.66TILLEE6 pKa = 4.16NEE8 pKa = 3.85CDD10 pKa = 3.18IDD12 pKa = 5.19LGIDD16 pKa = 3.43YY17 pKa = 7.36EE18 pKa = 4.76TIATRR23 pKa = 11.84VIEE26 pKa = 4.13AALDD30 pKa = 3.96YY31 pKa = 9.7VQCPYY36 pKa = 10.4EE37 pKa = 4.52CEE39 pKa = 4.17VNIVLTDD46 pKa = 3.38NDD48 pKa = 4.7GIQTVNAQTRR58 pKa = 11.84QINAPTDD65 pKa = 3.54VLSFPMIDD73 pKa = 4.37FEE75 pKa = 4.65TEE77 pKa = 3.25ADD79 pKa = 4.02FSIVEE84 pKa = 4.23HH85 pKa = 7.37DD86 pKa = 3.44EE87 pKa = 4.08ASYY90 pKa = 11.23FDD92 pKa = 4.03AATGEE97 pKa = 4.29LLLGDD102 pKa = 4.36IMISLDD108 pKa = 3.68KK109 pKa = 11.26VKK111 pKa = 10.49EE112 pKa = 3.61QAKK115 pKa = 10.14AYY117 pKa = 7.81NHH119 pKa = 5.56SVIRR123 pKa = 11.84EE124 pKa = 3.9YY125 pKa = 11.57AFLLAHH131 pKa = 6.71SMLHH135 pKa = 6.12LSGYY139 pKa = 10.38DD140 pKa = 3.2HH141 pKa = 6.67MVDD144 pKa = 3.37EE145 pKa = 4.65EE146 pKa = 4.65RR147 pKa = 11.84IRR149 pKa = 11.84MEE151 pKa = 3.97QMQDD155 pKa = 3.46EE156 pKa = 4.62ILQSIGYY163 pKa = 7.22TRR165 pKa = 11.84DD166 pKa = 3.23YY167 pKa = 11.37TEE169 pKa = 3.7

Molecular weight: 19.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5EGB6|R5EGB6_9CLOT Aspartokinase OS=Clostridium sp. CAG:590 OX=1262825 GN=BN724_01583 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.74VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2354

0

2354

755942

30

1954

321.1

36.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.194 ± 0.049

1.433 ± 0.018

6.043 ± 0.044

7.48 ± 0.056

3.845 ± 0.035

6.721 ± 0.042

1.738 ± 0.022

7.658 ± 0.05

7.107 ± 0.049

8.539 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.238 ± 0.027

4.604 ± 0.042

2.987 ± 0.025

3.526 ± 0.028

4.026 ± 0.036

5.604 ± 0.042

5.694 ± 0.053

7.284 ± 0.042

0.797 ± 0.016

4.476 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski