Escherichia phage phi191

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sepvirinae; Oslovirus; Escherichia virus 191

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A096XEP6|A0A096XEP6_9CAUD Uncharacterized protein OS=Escherichia phage phi191 OX=1458706 GN=phi191_00034 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 6.02DD3 pKa = 4.69KK4 pKa = 11.06IIAYY8 pKa = 8.38FVVLSDD14 pKa = 3.63EE15 pKa = 4.99AISVIDD21 pKa = 3.53TEE23 pKa = 4.75GNVMFIAEE31 pKa = 4.49HH32 pKa = 6.78PEE34 pKa = 4.56DD35 pKa = 3.76IALQAAAYY43 pKa = 8.68FFAKK47 pKa = 9.82EE48 pKa = 3.91QEE50 pKa = 4.37EE51 pKa = 4.59EE52 pKa = 4.71CNCPVCQLSRR62 pKa = 11.84QINLMHH68 pKa = 7.14

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A096XER0|A0A096XER0_9CAUD Uncharacterized protein OS=Escherichia phage phi191 OX=1458706 GN=phi191_00041 PE=4 SV=1
MM1 pKa = 7.11KK2 pKa = 10.36QIAFYY7 pKa = 10.4RR8 pKa = 11.84RR9 pKa = 11.84SGRR12 pKa = 11.84PGAFRR17 pKa = 11.84GLKK20 pKa = 9.98EE21 pKa = 3.78RR22 pKa = 11.84VTWMIQSRR30 pKa = 11.84GRR32 pKa = 11.84PVTGSEE38 pKa = 3.59IAEE41 pKa = 4.12KK42 pKa = 10.53FGVSLCEE49 pKa = 3.92FNKK52 pKa = 9.89VARR55 pKa = 11.84GLTKK59 pKa = 9.93GSKK62 pKa = 8.32VVKK65 pKa = 10.17IKK67 pKa = 10.72ASEE70 pKa = 4.1PFTTDD75 pKa = 2.19TGIVDD80 pKa = 3.88RR81 pKa = 11.84FFSLEE86 pKa = 4.02SNPRR90 pKa = 11.84RR91 pKa = 11.84DD92 pKa = 3.57TPRR95 pKa = 11.84SRR97 pKa = 11.84NAVPPFSRR105 pKa = 11.84RR106 pKa = 11.84SRR108 pKa = 11.84EE109 pKa = 3.82HH110 pKa = 5.97AAKK113 pKa = 10.17NCRR116 pKa = 11.84EE117 pKa = 4.05EE118 pKa = 4.13YY119 pKa = 8.72VQKK122 pKa = 10.9AEE124 pKa = 3.91RR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84LIKK131 pKa = 10.2AGLYY135 pKa = 9.58IDD137 pKa = 4.18EE138 pKa = 5.21FEE140 pKa = 4.32NALL143 pKa = 3.75

Molecular weight: 16.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

16636

38

699

191.2

21.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.702 ± 0.413

1.136 ± 0.145

5.572 ± 0.181

6.498 ± 0.265

3.468 ± 0.232

7.532 ± 0.605

1.779 ± 0.13

4.959 ± 0.238

5.735 ± 0.225

7.802 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.795 ± 0.157

4.394 ± 0.167

4.532 ± 0.3

4.202 ± 0.263

6.66 ± 0.286

6.336 ± 0.267

5.933 ± 0.23

6.678 ± 0.273

1.328 ± 0.109

2.831 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski