Microbacterium sp. ZXX196

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2672 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I3LAB8|A0A6I3LAB8_9MICO 50S ribosomal protein L36 OS=Microbacterium sp. ZXX196 OX=2609291 GN=rpmJ PE=3 SV=1
MM1 pKa = 8.12RR2 pKa = 11.84LTTPLAAAALAGALVLTSCSSSQNPGPDD30 pKa = 3.11EE31 pKa = 4.74GGVAQADD38 pKa = 3.39ATIYY42 pKa = 10.6VGSLYY47 pKa = 10.49EE48 pKa = 4.16PQNLDD53 pKa = 3.24NTSGGGQGVTEE64 pKa = 4.27ALTGNVYY71 pKa = 10.62EE72 pKa = 4.32GLYY75 pKa = 10.48RR76 pKa = 11.84LADD79 pKa = 3.72SGEE82 pKa = 4.11VEE84 pKa = 4.36PLLAAAHH91 pKa = 5.84EE92 pKa = 4.26VSEE95 pKa = 5.53DD96 pKa = 3.01GLTYY100 pKa = 10.66TFTLQDD106 pKa = 3.44GVSFASGKK114 pKa = 8.06TLTADD119 pKa = 3.25DD120 pKa = 3.84VVYY123 pKa = 10.88SIEE126 pKa = 4.73KK127 pKa = 10.44VIADD131 pKa = 4.21DD132 pKa = 3.72SQSARR137 pKa = 11.84KK138 pKa = 8.82SQLEE142 pKa = 4.4VIDD145 pKa = 4.13SVAAVDD151 pKa = 3.91DD152 pKa = 3.5QTVRR156 pKa = 11.84VTLSEE161 pKa = 3.94RR162 pKa = 11.84SISFLYY168 pKa = 9.67NLSYY172 pKa = 11.06VWIVNSAFEE181 pKa = 4.46GNRR184 pKa = 11.84SSEE187 pKa = 4.14ADD189 pKa = 3.1GTGPYY194 pKa = 10.56VLDD197 pKa = 2.97TWTRR201 pKa = 11.84GSALSLVRR209 pKa = 11.84NDD211 pKa = 4.95DD212 pKa = 3.58YY213 pKa = 11.25WGEE216 pKa = 3.92APANGGVVFTYY227 pKa = 10.58FSDD230 pKa = 3.58ATSLNNALLTGAVDD244 pKa = 3.79VVTSQQSPDD253 pKa = 2.99GLAQFEE259 pKa = 4.38QTDD262 pKa = 4.13DD263 pKa = 3.66FTITDD268 pKa = 3.87GNSTTKK274 pKa = 10.89LLLAFNDD281 pKa = 3.49AAAPFDD287 pKa = 4.02DD288 pKa = 3.58VRR290 pKa = 11.84VRR292 pKa = 11.84QAVSAAIDD300 pKa = 3.73DD301 pKa = 4.26EE302 pKa = 4.54KK303 pKa = 11.24LLEE306 pKa = 4.52SVWGDD311 pKa = 3.37YY312 pKa = 8.14GTLIGSMVPPTDD324 pKa = 2.97PWFEE328 pKa = 4.66DD329 pKa = 3.47LTDD332 pKa = 4.81LDD334 pKa = 4.35TYY336 pKa = 11.57DD337 pKa = 4.25PDD339 pKa = 3.78RR340 pKa = 11.84AADD343 pKa = 3.99LLAEE347 pKa = 4.72AGYY350 pKa = 11.07EE351 pKa = 4.09DD352 pKa = 5.53GFTFTLDD359 pKa = 3.3TPNYY363 pKa = 9.21DD364 pKa = 3.72PHH366 pKa = 6.24PTAATFIQSQLAEE379 pKa = 3.88VGITVEE385 pKa = 4.26INTITSDD392 pKa = 2.67EE393 pKa = 4.85WYY395 pKa = 9.44TKK397 pKa = 10.26VYY399 pKa = 9.93QNRR402 pKa = 11.84DD403 pKa = 3.31FEE405 pKa = 4.61ATMQEE410 pKa = 4.31HH411 pKa = 6.41VNDD414 pKa = 5.12RR415 pKa = 11.84DD416 pKa = 3.88LVWYY420 pKa = 8.66GNPDD424 pKa = 4.11FYY426 pKa = 10.72WGYY429 pKa = 11.4DD430 pKa = 3.3NAEE433 pKa = 3.99VQEE436 pKa = 4.23WVAQSEE442 pKa = 4.69LVSSEE447 pKa = 4.3AEE449 pKa = 3.82QTEE452 pKa = 4.19LLKK455 pKa = 11.08LVARR459 pKa = 11.84TTAEE463 pKa = 3.86EE464 pKa = 4.29AASEE468 pKa = 3.99WLYY471 pKa = 10.8LYY473 pKa = 9.17PQIVVASSALSGYY486 pKa = 8.67PVNGLNSQFFAYY498 pKa = 10.2DD499 pKa = 3.19IEE501 pKa = 4.48KK502 pKa = 10.66AAA504 pKa = 4.43

Molecular weight: 54.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I3LDZ8|A0A6I3LDZ8_9MICO DUF368 domain-containing protein OS=Microbacterium sp. ZXX196 OX=2609291 GN=GJQ66_03800 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2672

0

2672

793030

22

2073

296.8

31.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.316 ± 0.088

0.503 ± 0.012

6.326 ± 0.05

6.063 ± 0.044

3.136 ± 0.026

9.104 ± 0.045

1.928 ± 0.022

4.435 ± 0.033

1.95 ± 0.032

9.708 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.832 ± 0.021

1.847 ± 0.025

5.282 ± 0.035

2.677 ± 0.027

7.662 ± 0.067

5.193 ± 0.035

5.712 ± 0.03

8.897 ± 0.042

1.472 ± 0.022

1.958 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski