Streptomyces tsukubensis (strain DSM 42081 / NBRC 108919 / NRRL 18488 / 9993)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces tsukubensis

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6304 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2MW45|I2MW45_STRT9 Peptidase OS=Streptomyces tsukubensis (strain DSM 42081 / NBRC 108919 / NRRL 18488 / 9993) OX=1114943 GN=STSU_028025 PE=4 SV=1
MM1 pKa = 7.2TVQHH5 pKa = 6.31EE6 pKa = 4.51APSPEE11 pKa = 3.78AAEE14 pKa = 4.27ALEE17 pKa = 3.98VWIDD21 pKa = 3.41QDD23 pKa = 3.99LCTGDD28 pKa = 5.01GICAQYY34 pKa = 11.17APDD37 pKa = 4.08VFEE40 pKa = 6.18LDD42 pKa = 3.22IDD44 pKa = 3.57GLAYY48 pKa = 10.42VKK50 pKa = 10.8SADD53 pKa = 4.52DD54 pKa = 4.1EE55 pKa = 4.59LLQEE59 pKa = 4.84PGATTPVPLPLLRR72 pKa = 11.84DD73 pKa = 3.74VVDD76 pKa = 4.03SAKK79 pKa = 10.21EE80 pKa = 3.92CPGDD84 pKa = 4.18CIHH87 pKa = 6.28VRR89 pKa = 11.84RR90 pKa = 11.84VSDD93 pKa = 3.47SVEE96 pKa = 4.14VYY98 pKa = 10.93GPDD101 pKa = 3.69AEE103 pKa = 4.31

Molecular weight: 11.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2N2N5|I2N2N5_STRT9 Putative DNA-binding protein OS=Streptomyces tsukubensis (strain DSM 42081 / NBRC 108919 / NRRL 18488 / 9993) OX=1114943 GN=STSU_016610 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.86GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6304

0

6304

2206834

27

11769

350.1

37.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.88 ± 0.047

0.762 ± 0.009

5.925 ± 0.025

5.663 ± 0.029

2.618 ± 0.018

9.943 ± 0.039

2.23 ± 0.016

3.079 ± 0.022

1.951 ± 0.026

10.242 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.602 ± 0.013

1.678 ± 0.017

6.59 ± 0.039

2.497 ± 0.017

8.385 ± 0.039

4.91 ± 0.023

6.254 ± 0.03

8.316 ± 0.035

1.461 ± 0.013

2.014 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski