Synechococcus phage S-CBS2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4YCL3|F4YCL3_9CAUD Structural protein OS=Synechococcus phage S-CBS2 OX=753084 GN=S-CBS2_gp032 PE=4 SV=1
MM1 pKa = 7.63AVPSNRR7 pKa = 11.84VPVRR11 pKa = 11.84VARR14 pKa = 11.84GLKK17 pKa = 10.04SALTANLADD26 pKa = 4.16LLEE29 pKa = 5.37GEE31 pKa = 4.59LVYY34 pKa = 11.13AKK36 pKa = 10.76DD37 pKa = 3.53EE38 pKa = 4.7DD39 pKa = 3.97KK40 pKa = 11.33LYY42 pKa = 10.04MIEE45 pKa = 4.46GGTLVAMGADD55 pKa = 3.96LAAASILDD63 pKa = 4.14LGDD66 pKa = 3.71VEE68 pKa = 5.32PNSLPDD74 pKa = 3.44GARR77 pKa = 11.84YY78 pKa = 9.82ALGGVQFPGNGLWYY92 pKa = 10.07RR93 pKa = 11.84SGSRR97 pKa = 11.84ISWGAIDD104 pKa = 5.55ADD106 pKa = 3.96STDD109 pKa = 3.42RR110 pKa = 11.84TSQITAASGSIFWSTDD126 pKa = 2.43GATWNEE132 pKa = 3.73IFFTGSPQTVGDD144 pKa = 4.21SYY146 pKa = 11.7YY147 pKa = 10.24IDD149 pKa = 3.44ITSGSLPALTGDD161 pKa = 4.62LYY163 pKa = 11.35FSFSTPGTPSPAPLADD179 pKa = 3.49GDD181 pKa = 4.05YY182 pKa = 11.26LRR184 pKa = 11.84FDD186 pKa = 3.68GTDD189 pKa = 3.52FRR191 pKa = 11.84PQQLATVAEE200 pKa = 4.39TGAYY204 pKa = 9.7SDD206 pKa = 5.03LSGTPTLVEE215 pKa = 4.8NIDD218 pKa = 4.18DD219 pKa = 4.12LTDD222 pKa = 3.25VDD224 pKa = 4.57TSTSAPTTDD233 pKa = 5.61QVLAWNGTNWVPSDD247 pKa = 3.42VSAVGGGVTRR257 pKa = 11.84IQDD260 pKa = 3.39ATDD263 pKa = 3.59YY264 pKa = 10.69EE265 pKa = 5.05PNTTPPAGTIYY276 pKa = 10.61YY277 pKa = 9.82FSKK280 pKa = 10.95AIANFPEE287 pKa = 5.25DD288 pKa = 3.35WTCPDD293 pKa = 3.48RR294 pKa = 11.84GTYY297 pKa = 8.51STDD300 pKa = 3.31PGDD303 pKa = 3.61GTQYY307 pKa = 11.62NLARR311 pKa = 11.84YY312 pKa = 9.48DD313 pKa = 3.48RR314 pKa = 11.84DD315 pKa = 3.52GRR317 pKa = 11.84DD318 pKa = 3.46TQSVFPSSGSIWWTFDD334 pKa = 2.88LTQPWTQMTYY344 pKa = 9.99TGLGAQSICTANSRR358 pKa = 11.84KK359 pKa = 10.11AYY361 pKa = 10.28NGTVNGWPLDD371 pKa = 3.48GSTDD375 pKa = 3.56KK376 pKa = 10.85ILYY379 pKa = 9.13VALSDD384 pKa = 4.25LGTGIPIPLAAGDD397 pKa = 3.81YY398 pKa = 10.45LRR400 pKa = 11.84YY401 pKa = 10.32DD402 pKa = 3.71GGDD405 pKa = 3.5FRR407 pKa = 11.84PKK409 pKa = 10.18QLATVAEE416 pKa = 4.39TGAYY420 pKa = 9.74SDD422 pKa = 4.92LSGAPTLGTAAAAATTDD439 pKa = 3.71FATAAQGTLADD450 pKa = 4.26SAVQPTDD457 pKa = 4.55SIDD460 pKa = 3.35TLADD464 pKa = 3.39VTVTTPSNGEE474 pKa = 3.72ALVWNGSAWVNSTVSTVGSIDD495 pKa = 4.39DD496 pKa = 4.16LTDD499 pKa = 2.95VDD501 pKa = 4.5TTTSAPTNGQVLEE514 pKa = 4.37WNGTNWVPATIAAGGVTSIIAGTGISVDD542 pKa = 3.42QATGDD547 pKa = 3.57VTITATGGGVSGIGVATRR565 pKa = 11.84VSDD568 pKa = 3.48SGTAASGALALTGLGSSGMLHH589 pKa = 6.02TLSTDD594 pKa = 3.06LDD596 pKa = 3.65AWVVFYY602 pKa = 10.85GSAADD607 pKa = 3.93RR608 pKa = 11.84TADD611 pKa = 3.62AGRR614 pKa = 11.84AYY616 pKa = 9.92NTDD619 pKa = 3.53PAAGSGVLAEE629 pKa = 4.98AYY631 pKa = 7.75VTTAGAILFTPGTGYY646 pKa = 10.26MNNDD650 pKa = 3.26TTEE653 pKa = 3.82TAALYY658 pKa = 10.42LAVRR662 pKa = 11.84DD663 pKa = 3.85QAGAAVNATLTVTAYY678 pKa = 10.62VQGGYY683 pKa = 10.62DD684 pKa = 3.63GVSGGTFGSGG694 pKa = 2.79

Molecular weight: 71.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4YCP0|F4YCP0_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBS2 OX=753084 GN=S-CBS2_gp059 PE=4 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84LSHH5 pKa = 6.25QEE7 pKa = 3.49AEE9 pKa = 4.46ARR11 pKa = 11.84RR12 pKa = 11.84QLYY15 pKa = 10.18EE16 pKa = 4.05EE17 pKa = 4.54GGKK20 pKa = 10.51AIGEE24 pKa = 4.24LLCTYY29 pKa = 9.73PEE31 pKa = 4.68KK32 pKa = 10.75EE33 pKa = 3.94PQIWKK38 pKa = 9.68LVAGAGLWTRR48 pKa = 11.84KK49 pKa = 9.9DD50 pKa = 3.09LAAFKK55 pKa = 10.53SLWRR59 pKa = 11.84RR60 pKa = 11.84DD61 pKa = 3.48HH62 pKa = 5.97PVEE65 pKa = 3.79YY66 pKa = 10.75SRR68 pKa = 11.84IKK70 pKa = 8.61QARR73 pKa = 11.84YY74 pKa = 9.66DD75 pKa = 3.81RR76 pKa = 11.84KK77 pKa = 9.29QLARR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84AAKK86 pKa = 10.32LEE88 pKa = 3.94KK89 pKa = 9.71TRR91 pKa = 11.84EE92 pKa = 3.97ASKK95 pKa = 9.58PQAMPTEE102 pKa = 4.05RR103 pKa = 11.84RR104 pKa = 11.84PRR106 pKa = 11.84KK107 pKa = 9.61DD108 pKa = 3.1LRR110 pKa = 11.84DD111 pKa = 3.61RR112 pKa = 11.84KK113 pKa = 10.46

Molecular weight: 13.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

21656

43

956

212.3

23.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.656 ± 0.327

1.261 ± 0.274

6.008 ± 0.17

6.248 ± 0.321

3.325 ± 0.146

9.138 ± 0.525

1.598 ± 0.169

5.042 ± 0.18

4.738 ± 0.334

8.016 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.912 ± 0.14

4.248 ± 0.198

4.308 ± 0.203

4.059 ± 0.246

5.052 ± 0.311

6.968 ± 0.335

6.686 ± 0.414

6.516 ± 0.226

1.635 ± 0.106

3.588 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski