Roseofilum reptotaenium AO1-A

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Coleofasciculaceae; Roseofilum; Roseofilum reptotaenium

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4956 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L9QXH2|A0A1L9QXH2_9CYAN Uncharacterized protein OS=Roseofilum reptotaenium AO1-A OX=1925591 GN=BI308_02255 PE=4 SV=1
MM1 pKa = 6.96SQEE4 pKa = 3.98FINRR8 pKa = 11.84VVEE11 pKa = 3.79LTNFQRR17 pKa = 11.84SQFGLAPLVVNAQLTQAAQSHH38 pKa = 5.18TEE40 pKa = 3.66NMAFQDD46 pKa = 4.32FFSHH50 pKa = 6.55TGLDD54 pKa = 3.32NSSIGDD60 pKa = 3.79RR61 pKa = 11.84ISATGYY67 pKa = 9.03QASTGAEE74 pKa = 3.95NIAAGYY80 pKa = 6.62QTPEE84 pKa = 3.87QVVEE88 pKa = 3.95GWMNSPGHH96 pKa = 6.56RR97 pKa = 11.84ANILDD102 pKa = 4.61ANLQEE107 pKa = 4.44IGVGYY112 pKa = 9.83FFLANDD118 pKa = 4.02TGTVNYY124 pKa = 10.31NSYY127 pKa = 6.9WTQVFGTPRR136 pKa = 11.84NGVVTPAPAPTLVNPTSPFTLGSDD160 pKa = 5.04LNDD163 pKa = 3.15QMTGTVSNDD172 pKa = 3.3TIFALGGDD180 pKa = 4.45DD181 pKa = 3.47IARR184 pKa = 11.84GNSGHH189 pKa = 7.41DD190 pKa = 3.45YY191 pKa = 11.46VNGNLGNDD199 pKa = 3.47QVAGDD204 pKa = 4.82LGNDD208 pKa = 3.63SVRR211 pKa = 11.84GGQGNDD217 pKa = 3.14FVFGDD222 pKa = 3.8RR223 pKa = 11.84QNDD226 pKa = 3.46QVFGNNGDD234 pKa = 4.35DD235 pKa = 3.76ILYY238 pKa = 10.52GGQNEE243 pKa = 4.81DD244 pKa = 4.11YY245 pKa = 11.02LDD247 pKa = 4.09GGAGNDD253 pKa = 3.45ILYY256 pKa = 10.67GDD258 pKa = 5.01LGSDD262 pKa = 3.63VVIGGTGNDD271 pKa = 3.0IFVLRR276 pKa = 11.84PGAPDD281 pKa = 3.35LMFYY285 pKa = 11.17NDD287 pKa = 3.56AEE289 pKa = 4.48DD290 pKa = 4.34YY291 pKa = 10.89IGLTGGLSFNSLTFNSGSGEE311 pKa = 4.06LANATLIYY319 pKa = 10.2QAGTTNVLAILPNVAPSQIDD339 pKa = 3.24PGDD342 pKa = 4.18FILVV346 pKa = 3.56

Molecular weight: 36.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L9QV79|A0A1L9QV79_9CYAN Mg-protoporphyrin IX chelatase OS=Roseofilum reptotaenium AO1-A OX=1925591 GN=BI308_05730 PE=3 SV=1
MM1 pKa = 7.79SKK3 pKa = 9.03RR4 pKa = 11.84TLEE7 pKa = 3.95GTNRR11 pKa = 11.84KK12 pKa = 7.62QKK14 pKa = 8.83RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84SNTGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84TRR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.17KK38 pKa = 9.81GRR40 pKa = 11.84HH41 pKa = 5.26RR42 pKa = 11.84LTVV45 pKa = 3.07

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4956

0

4956

1620154

33

3921

326.9

36.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.459 ± 0.031

1.017 ± 0.01

5.148 ± 0.026

6.887 ± 0.036

3.809 ± 0.023

6.723 ± 0.043

2.028 ± 0.018

6.597 ± 0.027

4.371 ± 0.038

11.271 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.956 ± 0.016

3.995 ± 0.028

4.965 ± 0.03

5.729 ± 0.033

5.202 ± 0.027

6.454 ± 0.026

5.493 ± 0.029

6.247 ± 0.024

1.537 ± 0.017

3.113 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski