Arthrobacter agilis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2887 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y3LYI9|A0A1Y3LYI9_9MICC Cholesterol dehydrogenase OS=Arthrobacter agilis OX=37921 GN=B8W74_12855 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.55AKK4 pKa = 10.05FLASAGIVAATAVALTGCSGGNGGGDD30 pKa = 3.31GGGSEE35 pKa = 5.13AASCTNTIVNAEE47 pKa = 4.06APQVTVWAWYY57 pKa = 8.09PAFDD61 pKa = 4.2QVVDD65 pKa = 3.79NFNNANDD72 pKa = 4.22DD73 pKa = 4.28VQICWTNAGQGNDD86 pKa = 3.61EE87 pKa = 4.16YY88 pKa = 10.85TKK90 pKa = 10.57FSTSIEE96 pKa = 4.43SGSGAPDD103 pKa = 3.87VIQLEE108 pKa = 4.61SEE110 pKa = 4.4VLSSFTIRR118 pKa = 11.84DD119 pKa = 3.45ALVDD123 pKa = 3.76LTEE126 pKa = 4.2YY127 pKa = 10.55GANDD131 pKa = 3.37VEE133 pKa = 4.28GDD135 pKa = 3.81YY136 pKa = 11.77AEE138 pKa = 5.0GAWEE142 pKa = 4.32DD143 pKa = 3.96ASSGDD148 pKa = 3.44AVYY151 pKa = 10.35AIPVDD156 pKa = 4.16GGPMGMLYY164 pKa = 10.34RR165 pKa = 11.84QDD167 pKa = 3.34IFDD170 pKa = 3.87AAGIEE175 pKa = 4.71VPTTWEE181 pKa = 3.85EE182 pKa = 3.88FEE184 pKa = 3.97AAARR188 pKa = 11.84TLKK191 pKa = 10.89DD192 pKa = 3.37SGDD195 pKa = 3.68GAVIADD201 pKa = 4.62FPTNGRR207 pKa = 11.84AFNQALFAQAGSEE220 pKa = 4.01PFVYY224 pKa = 10.49DD225 pKa = 3.13SANPQEE231 pKa = 4.57IGITVNDD238 pKa = 4.18DD239 pKa = 3.29GAKK242 pKa = 10.08KK243 pKa = 10.32VLEE246 pKa = 4.13YY247 pKa = 10.71WNGLVEE253 pKa = 5.45DD254 pKa = 4.77GLVATDD260 pKa = 4.26DD261 pKa = 4.86AFTADD266 pKa = 4.2YY267 pKa = 7.76NTKK270 pKa = 10.53LVDD273 pKa = 3.63GSYY276 pKa = 11.06AVYY279 pKa = 10.1LAAAWGPGYY288 pKa = 10.47LQGLSDD294 pKa = 4.5SDD296 pKa = 4.17PDD298 pKa = 4.22AVWRR302 pKa = 11.84AAPLPLWDD310 pKa = 4.08EE311 pKa = 4.45ANPVQVNWGGSTFAVTEE328 pKa = 3.77QAEE331 pKa = 4.47NKK333 pKa = 9.46EE334 pKa = 3.84LAARR338 pKa = 11.84VAMEE342 pKa = 3.78IFGNEE347 pKa = 3.19EE348 pKa = 3.76RR349 pKa = 11.84MNLGVDD355 pKa = 3.8EE356 pKa = 4.91GALFPSYY363 pKa = 10.88LPVLEE368 pKa = 4.55SPEE371 pKa = 4.19FEE373 pKa = 3.96NKK375 pKa = 9.72EE376 pKa = 3.87YY377 pKa = 10.97EE378 pKa = 4.34FFGGQQINKK387 pKa = 9.99DD388 pKa = 3.74VFLDD392 pKa = 3.84AAAGYY397 pKa = 9.57EE398 pKa = 4.19GATFSPFQNYY408 pKa = 10.17AYY410 pKa = 10.26DD411 pKa = 3.58QLTEE415 pKa = 3.82QIYY418 pKa = 11.32AMVQGEE424 pKa = 4.19KK425 pKa = 10.71DD426 pKa = 3.12ADD428 pKa = 3.6QALDD432 pKa = 4.0DD433 pKa = 4.68LQSSLEE439 pKa = 4.06QYY441 pKa = 9.34ATEE444 pKa = 3.81QGFTIKK450 pKa = 10.67

Molecular weight: 48.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y3LV39|A0A1Y3LV39_9MICC Transcriptional regulator MalT OS=Arthrobacter agilis OX=37921 GN=B8W74_13310 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2887

0

2887

945779

26

1816

327.6

35.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.011 ± 0.061

0.571 ± 0.011

6.185 ± 0.037

5.64 ± 0.041

3.089 ± 0.027

9.246 ± 0.042

2.036 ± 0.022

4.193 ± 0.034

2.032 ± 0.033

10.329 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.836 ± 0.018

2.065 ± 0.024

5.472 ± 0.034

2.964 ± 0.022

7.2 ± 0.053

5.951 ± 0.033

6.166 ± 0.033

8.673 ± 0.042

1.344 ± 0.018

1.995 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski