Kitasatospora viridis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Kitasatospora

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8776 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A561UES7|A0A561UES7_9ACTN Maltokinase OS=Kitasatospora viridis OX=281105 GN=FHX73_111657 PE=3 SV=1
MM1 pKa = 7.59TGTTLSADD9 pKa = 3.79DD10 pKa = 3.54QGEE13 pKa = 3.95FLDD16 pKa = 4.26RR17 pKa = 11.84FSHH20 pKa = 7.01FEE22 pKa = 4.21DD23 pKa = 4.56GVITGIRR30 pKa = 11.84LQLPRR35 pKa = 11.84TGGSGWTAEE44 pKa = 4.25FDD46 pKa = 3.78VQAVDD51 pKa = 4.66GADD54 pKa = 3.42GDD56 pKa = 3.56EE57 pKa = 3.94WRR59 pKa = 11.84LVRR62 pKa = 11.84ITLSGLYY69 pKa = 9.52EE70 pKa = 4.08YY71 pKa = 9.84EE72 pKa = 4.38FPLSRR77 pKa = 11.84QYY79 pKa = 11.62SYY81 pKa = 11.46FVLSDD86 pKa = 4.04GLNLDD91 pKa = 4.15CTAEE95 pKa = 4.03RR96 pKa = 11.84CLLDD100 pKa = 5.18LDD102 pKa = 5.13PGPDD106 pKa = 3.36PWTPAQVGQPGEE118 pKa = 3.99YY119 pKa = 9.64SKK121 pKa = 11.08QYY123 pKa = 11.13AIGTSCTYY131 pKa = 10.3EE132 pKa = 4.07VLDD135 pKa = 4.79GPFII139 pKa = 4.51

Molecular weight: 15.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A561UHH9|A0A561UHH9_9ACTN MFS transporter OS=Kitasatospora viridis OX=281105 GN=FHX73_112648 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.43GRR40 pKa = 11.84SSISAA45 pKa = 3.4

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8776

0

8776

2985417

28

8237

340.2

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.485 ± 0.042

0.802 ± 0.007

5.602 ± 0.019

5.36 ± 0.031

2.666 ± 0.013

9.605 ± 0.029

2.29 ± 0.014

2.841 ± 0.018

1.612 ± 0.019

11.143 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.497 ± 0.012

1.783 ± 0.019

6.495 ± 0.027

3.206 ± 0.017

7.818 ± 0.035

4.923 ± 0.024

6.138 ± 0.031

8.175 ± 0.026

1.554 ± 0.012

2.006 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski