Alcelaphine herpesvirus 1 (strain C500) (AlHV-1) (Malignant catarrhal fever virus)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Gammaherpesvirinae; Macavirus; Alcelaphine gammaherpesvirus 1

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|O36360|DNBI_ALHV1 Major DNA-binding protein OS=Alcelaphine herpesvirus 1 (strain C500) OX=654901 GN=DBP PE=3 SV=1
MM1 pKa = 7.21VAQLYY6 pKa = 9.75HH7 pKa = 6.95HH8 pKa = 6.73LTILIASIYY17 pKa = 10.37IIFFVNAAPTLYY29 pKa = 10.54EE30 pKa = 5.04DD31 pKa = 5.46DD32 pKa = 5.05EE33 pKa = 4.96EE34 pKa = 5.52DD35 pKa = 4.37FNSTEE40 pKa = 3.81YY41 pKa = 11.19DD42 pKa = 3.43VFLDD46 pKa = 3.32SDD48 pKa = 3.82SFQFPRR54 pKa = 11.84RR55 pKa = 11.84NSSCNCTRR63 pKa = 11.84EE64 pKa = 3.92EE65 pKa = 3.94ATRR68 pKa = 11.84TLLEE72 pKa = 3.95ALKK75 pKa = 10.53RR76 pKa = 11.84ALQIIAGYY84 pKa = 10.18INEE87 pKa = 4.52TDD89 pKa = 3.61TEE91 pKa = 4.31EE92 pKa = 4.6LPTYY96 pKa = 8.86PPTMTTPLEE105 pKa = 4.2TTPLDD110 pKa = 3.39TSPPVLPSAIPP121 pKa = 3.31

Molecular weight:
13.69 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|O36416|UL49_ALHV1 Uncharacterized gene 66 protein OS=Alcelaphine herpesvirus 1 (strain C500) OX=654901 GN=66 PE=3 SV=1
MM1 pKa = 7.12AHH3 pKa = 7.11ARR5 pKa = 11.84PKK7 pKa = 10.49LPRR10 pKa = 11.84INLRR14 pKa = 11.84LDD16 pKa = 3.19AEE18 pKa = 4.55YY19 pKa = 10.24PNDD22 pKa = 3.45PRR24 pKa = 11.84VQQLHH29 pKa = 5.79LQILNNPNYY38 pKa = 9.8ANNVRR43 pKa = 11.84APLSYY48 pKa = 10.88LVFLTAQEE56 pKa = 4.14MYY58 pKa = 10.68EE59 pKa = 4.22VFVRR63 pKa = 11.84QARR66 pKa = 11.84GVNKK70 pKa = 9.6KK71 pKa = 10.43RR72 pKa = 11.84SGGPEE77 pKa = 4.03KK78 pKa = 10.44PILPANPGAGPQNAPGCGGGGSGSGGSGPPGGSAPQIQPPNNGVPSNQVSSNSGSAAGSGSGSGASSNSGAGSGAGAGSSACPSSGAGSGAGAGSSAGSNVQAPPAPPPLPQVPMVPGVVNGQEE202 pKa = 4.18MYY204 pKa = 11.08LPLGPVNGLPQQLGTAGSDD223 pKa = 3.27NSGGQASSPGSQNPPTKK240 pKa = 10.06PVAPRR245 pKa = 11.84GKK247 pKa = 9.96LGKK250 pKa = 10.43GRR252 pKa = 3.76

Molecular weight:
24.55 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

34799

59

2606

497.1

55.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.313 ± 0.276

2.624 ± 0.19

4.647 ± 0.171

5.828 ± 0.606

4.793 ± 0.211

5.742 ± 0.964

2.489 ± 0.119

5.178 ± 0.219

5.296 ± 0.224

10.311 ± 0.391

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.121 ± 0.107

4.92 ± 0.23

5.974 ± 0.449

4.25 ± 0.222

3.928 ± 0.202

8.316 ± 0.251

6.29 ± 0.273

6.423 ± 0.241

1.06 ± 0.066

3.497 ± 0.167

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski