Quaranfil virus (isolate QrfV/Tick/Afghanistan/EG_T_377/1968) (QRFV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Quaranjavirus; Quaranfil quaranjavirus

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|D0UFC9|M1_QRFVE Probable matrix protein OS=Quaranfil virus (isolate QrfV/Tick/Afghanistan/EG_T_377/1968) OX=1559362 GN=Segment 6 PE=4 SV=1
MM1 pKa = 7.4AFEE4 pKa = 5.3AYY6 pKa = 9.91RR7 pKa = 11.84LLIANPQLYY16 pKa = 9.98EE17 pKa = 4.13PDD19 pKa = 3.81FVSQAGEE26 pKa = 4.1LSEE29 pKa = 3.98HH30 pKa = 6.37WARR33 pKa = 11.84EE34 pKa = 3.45NWKK37 pKa = 10.16RR38 pKa = 11.84RR39 pKa = 11.84EE40 pKa = 3.51EE41 pKa = 4.18SLRR44 pKa = 11.84HH45 pKa = 6.34DD46 pKa = 4.3KK47 pKa = 11.27VCMLLMNTEE56 pKa = 3.83PRR58 pKa = 11.84EE59 pKa = 4.05RR60 pKa = 11.84SHH62 pKa = 5.81TQAEE66 pKa = 4.66GEE68 pKa = 4.36EE69 pKa = 4.53EE70 pKa = 4.53GSSSRR75 pKa = 11.84SLATEE80 pKa = 3.78EE81 pKa = 4.42GEE83 pKa = 4.64KK84 pKa = 10.55RR85 pKa = 11.84FSLEE89 pKa = 3.77ALEE92 pKa = 4.69GLVDD96 pKa = 3.5QGRR99 pKa = 11.84EE100 pKa = 3.82VLLEE104 pKa = 3.92NLEE107 pKa = 4.15MEE109 pKa = 4.57EE110 pKa = 4.06AQANSDD116 pKa = 3.86SEE118 pKa = 4.69GPNEE122 pKa = 4.2ADD124 pKa = 2.97SGGEE128 pKa = 3.89GEE130 pKa = 4.3EE131 pKa = 4.09FVFGVEE137 pKa = 4.6SEE139 pKa = 4.72SEE141 pKa = 4.09MEE143 pKa = 4.3VASEE147 pKa = 4.15VTKK150 pKa = 10.9LLEE153 pKa = 4.74PDD155 pKa = 5.25LIDD158 pKa = 4.0SNFRR162 pKa = 11.84YY163 pKa = 8.19TLLEE167 pKa = 4.12GVTSGKK173 pKa = 9.1FAQMEE178 pKa = 4.5FTALWGINTNNKK190 pKa = 8.17WDD192 pKa = 3.61LVDD195 pKa = 3.5RR196 pKa = 11.84VNRR199 pKa = 11.84KK200 pKa = 9.48LIEE203 pKa = 4.32VKK205 pKa = 9.45VTTRR209 pKa = 11.84PPTGVWEE216 pKa = 4.22EE217 pKa = 4.34VVAHH221 pKa = 7.04AEE223 pKa = 4.31GTDD226 pKa = 3.65PEE228 pKa = 4.86HH229 pKa = 6.36NGAYY233 pKa = 9.54IIHH236 pKa = 7.96DD237 pKa = 4.15DD238 pKa = 3.63LCGNFTPYY246 pKa = 10.95KK247 pKa = 9.69FGNISDD253 pKa = 4.08LPGWPSAQDD262 pKa = 3.44FPIRR266 pKa = 11.84RR267 pKa = 11.84HH268 pKa = 5.93AFLASYY274 pKa = 10.62GGVPSAGGIEE284 pKa = 4.23EE285 pKa = 5.3GLPAWDD291 pKa = 3.82DD292 pKa = 3.38VFMEE296 pKa = 4.76RR297 pKa = 11.84TRR299 pKa = 11.84NWVAPLWKK307 pKa = 9.82KK308 pKa = 10.93GPISVRR314 pKa = 11.84RR315 pKa = 11.84DD316 pKa = 3.09DD317 pKa = 4.32CPRR320 pKa = 11.84MEE322 pKa = 5.16PFNITKK328 pKa = 10.18FITLLEE334 pKa = 4.32DD335 pKa = 3.33PRR337 pKa = 11.84LRR339 pKa = 11.84NTDD342 pKa = 4.12KK343 pKa = 10.54SAKK346 pKa = 8.36WKK348 pKa = 10.84GKK350 pKa = 9.1ILPVSWCRR358 pKa = 11.84TIISSQEE365 pKa = 3.31KK366 pKa = 10.37DD367 pKa = 2.41IDD369 pKa = 3.74MVQEE373 pKa = 4.12VIRR376 pKa = 11.84DD377 pKa = 3.62LGVIGLWEE385 pKa = 4.87EE386 pKa = 4.09INANPVKK393 pKa = 10.77VEE395 pKa = 4.01RR396 pKa = 11.84TVSALSEE403 pKa = 3.99LMRR406 pKa = 11.84LFQEE410 pKa = 4.94GFRR413 pKa = 11.84EE414 pKa = 4.07KK415 pKa = 10.88NPYY418 pKa = 9.77ISVKK422 pKa = 9.83RR423 pKa = 11.84KK424 pKa = 9.06CKK426 pKa = 8.78GWVITGHH433 pKa = 5.98KK434 pKa = 7.31QTPALTDD441 pKa = 3.24TLLEE445 pKa = 4.56LGVGYY450 pKa = 10.43KK451 pKa = 9.84PYY453 pKa = 10.87QMSNVVEE460 pKa = 3.97EE461 pKa = 5.15DD462 pKa = 3.06MRR464 pKa = 11.84QCEE467 pKa = 3.92TDD469 pKa = 4.68DD470 pKa = 3.52IEE472 pKa = 4.62KK473 pKa = 10.07MKK475 pKa = 10.33WVDD478 pKa = 3.15WMSKK482 pKa = 9.83LAATEE487 pKa = 4.15SEE489 pKa = 4.12PLGSKK494 pKa = 9.89VSRR497 pKa = 11.84DD498 pKa = 3.96DD499 pKa = 3.16IFKK502 pKa = 9.76EE503 pKa = 3.94AEE505 pKa = 3.95SRR507 pKa = 11.84HH508 pKa = 5.4VLDD511 pKa = 4.76EE512 pKa = 4.28PAKK515 pKa = 9.63KK516 pKa = 9.02MCSKK520 pKa = 10.86VYY522 pKa = 10.62DD523 pKa = 4.32LYY525 pKa = 11.0RR526 pKa = 11.84SHH528 pKa = 7.57KK529 pKa = 10.37IGATCSKK536 pKa = 10.72FMGFYY541 pKa = 10.58SRR543 pKa = 11.84MGGSYY548 pKa = 10.47LRR550 pKa = 11.84AIAGNSRR557 pKa = 11.84QHH559 pKa = 6.22SSLAIMPLYY568 pKa = 10.23YY569 pKa = 9.22KK570 pKa = 9.95TYY572 pKa = 10.48QEE574 pKa = 5.26DD575 pKa = 4.18GTSTRR580 pKa = 11.84HH581 pKa = 4.52LTGLVIRR588 pKa = 11.84GPHH591 pKa = 5.92HH592 pKa = 6.27VRR594 pKa = 11.84EE595 pKa = 4.37STDD598 pKa = 3.51TINLLIVEE606 pKa = 4.61KK607 pKa = 9.44TCLSRR612 pKa = 11.84RR613 pKa = 11.84EE614 pKa = 4.09AQEE617 pKa = 3.88RR618 pKa = 11.84LSGGALVNGVWWVRR632 pKa = 11.84KK633 pKa = 8.74NAIRR637 pKa = 11.84KK638 pKa = 9.11ADD640 pKa = 3.58PTYY643 pKa = 11.01LAFLHH648 pKa = 6.51NSLFVPTNFLGEE660 pKa = 4.22LVTTPKK666 pKa = 10.53HH667 pKa = 6.08FLRR670 pKa = 11.84QRR672 pKa = 11.84QPRR675 pKa = 11.84ILGRR679 pKa = 11.84NPVWQLWLLL688 pKa = 3.93

Molecular weight: 78.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|G9HXM2|NCAP_QRFVE Nucleoprotein OS=Quaranfil virus (isolate QrfV/Tick/Afghanistan/EG_T_377/1968) OX=1559362 GN=Segment 4 PE=3 SV=1
MM1 pKa = 7.43EE2 pKa = 5.29VDD4 pKa = 4.5PAPSTAPKK12 pKa = 8.41PTRR15 pKa = 11.84KK16 pKa = 9.36RR17 pKa = 11.84VNNSGEE23 pKa = 4.21EE24 pKa = 3.82IGSGGKK30 pKa = 9.41RR31 pKa = 11.84ALTGLSLTQKK41 pKa = 10.2VAIHH45 pKa = 5.83HH46 pKa = 7.14AIALMVYY53 pKa = 9.66HH54 pKa = 5.99VVIDD58 pKa = 3.62IFKK61 pKa = 9.33CTIIDD66 pKa = 5.58LITDD70 pKa = 3.48VNIGVYY76 pKa = 10.71AMNQIWSIHH85 pKa = 5.46NYY87 pKa = 9.26FRR89 pKa = 11.84TKK91 pKa = 10.61NNSGRR96 pKa = 11.84SSIGAKK102 pKa = 9.28IDD104 pKa = 3.55NATDD108 pKa = 3.75TYY110 pKa = 11.41QFILHH115 pKa = 6.34GRR117 pKa = 11.84TITVNKK123 pKa = 10.06EE124 pKa = 3.72EE125 pKa = 4.33LKK127 pKa = 10.8GVYY130 pKa = 8.98KK131 pKa = 10.59RR132 pKa = 11.84CITTYY137 pKa = 10.83FDD139 pKa = 3.42WDD141 pKa = 4.3KK142 pKa = 11.12MNDD145 pKa = 3.09WMGSVHH151 pKa = 7.41TIMALWNLFGSRR163 pKa = 11.84LSEE166 pKa = 3.88VRR168 pKa = 11.84IMPSANTISVEE179 pKa = 3.77KK180 pKa = 10.59DD181 pKa = 2.71NRR183 pKa = 11.84TTVVKK188 pKa = 10.68DD189 pKa = 3.62FNQYY193 pKa = 10.48GIPAGMRR200 pKa = 11.84HH201 pKa = 5.99FATGADD207 pKa = 4.53FKK209 pKa = 10.69PTMKK213 pKa = 10.48SALAQSMGPVTVLVQLSEE231 pKa = 4.6AKK233 pKa = 10.45DD234 pKa = 3.39NQYY237 pKa = 11.0AGKK240 pKa = 9.27WVDD243 pKa = 3.04ALKK246 pKa = 10.54RR247 pKa = 11.84AFSHH251 pKa = 6.57IPHH254 pKa = 7.38IDD256 pKa = 3.4QIAKK260 pKa = 10.16FMLANKK266 pKa = 9.19PAVLTKK272 pKa = 10.45INGYY276 pKa = 10.55LMAIAGYY283 pKa = 8.67TGTRR287 pKa = 11.84EE288 pKa = 4.09QKK290 pKa = 10.54RR291 pKa = 11.84IAFPPGTLAYY301 pKa = 10.34LLHH304 pKa = 7.4DD305 pKa = 4.44LDD307 pKa = 5.5KK308 pKa = 11.32EE309 pKa = 4.4NKK311 pKa = 9.64LHH313 pKa = 6.83FNEE316 pKa = 4.74DD317 pKa = 3.32RR318 pKa = 11.84CSKK321 pKa = 10.52FDD323 pKa = 3.67FSGAGAFRR331 pKa = 11.84MYY333 pKa = 11.15SHH335 pKa = 7.54FLKK338 pKa = 10.59VCKK341 pKa = 10.22KK342 pKa = 10.29NPLRR346 pKa = 11.84MNIADD351 pKa = 4.04PGKK354 pKa = 9.76ARR356 pKa = 11.84QLLFHH361 pKa = 6.46TMFGTHH367 pKa = 5.81VEE369 pKa = 4.28DD370 pKa = 5.25FGILQSMTDD379 pKa = 3.03VSDD382 pKa = 3.09WLKK385 pKa = 11.0RR386 pKa = 11.84KK387 pKa = 10.21DD388 pKa = 3.58FEE390 pKa = 5.14EE391 pKa = 4.1EE392 pKa = 4.1FKK394 pKa = 10.56TLRR397 pKa = 11.84ASHH400 pKa = 6.34ARR402 pKa = 11.84VEE404 pKa = 4.65GNFYY408 pKa = 9.73PIEE411 pKa = 3.67MRR413 pKa = 11.84YY414 pKa = 9.01YY415 pKa = 10.86SKK417 pKa = 11.09VCSSLNTKK425 pKa = 9.66MIGGGSAPVTNCQIFSGNRR444 pKa = 11.84KK445 pKa = 9.74RR446 pKa = 11.84IVTEE450 pKa = 3.95GLKK453 pKa = 10.67SLGQQGVGAGLSALDD468 pKa = 3.72KK469 pKa = 11.37DD470 pKa = 4.73SIEE473 pKa = 4.01KK474 pKa = 10.56LLRR477 pKa = 11.84EE478 pKa = 4.04HH479 pKa = 7.03LEE481 pKa = 4.13SIKK484 pKa = 10.93AAFGDD489 pKa = 4.01SFEE492 pKa = 5.34AGTVKK497 pKa = 9.36WRR499 pKa = 11.84KK500 pKa = 9.21FDD502 pKa = 3.26GLTWDD507 pKa = 3.6KK508 pKa = 11.42EE509 pKa = 4.05GDD511 pKa = 3.85EE512 pKa = 4.49VEE514 pKa = 4.18MRR516 pKa = 11.84PGEE519 pKa = 3.95VGEE522 pKa = 4.92IYY524 pKa = 10.12WSNN527 pKa = 3.04

Molecular weight: 59.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3502

266

777

583.7

66.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.368 ± 0.4

1.571 ± 0.3

5.111 ± 0.29

7.453 ± 0.7

4.055 ± 0.256

6.91 ± 0.565

2.513 ± 0.195

4.712 ± 0.345

6.71 ± 0.308

9.338 ± 0.476

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.884 ± 0.207

4.397 ± 0.267

4.283 ± 0.4

3.055 ± 0.157

6.139 ± 0.525

6.853 ± 0.402

5.911 ± 0.169

6.425 ± 0.281

2.113 ± 0.243

3.198 ± 0.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski