Spirosoma montaniterrae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Spirosoma

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4811 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P9WWN2|A0A1P9WWN2_9BACT GMC family oxidoreductase OS=Spirosoma montaniterrae OX=1178516 GN=AWR27_10765 PE=4 SV=1
MM1 pKa = 7.38ATYY4 pKa = 10.28KK5 pKa = 10.95NEE7 pKa = 3.93GFDD10 pKa = 3.66PEE12 pKa = 4.83EE13 pKa = 4.23INALKK18 pKa = 10.51DD19 pKa = 3.08EE20 pKa = 4.68CRR22 pKa = 11.84QEE24 pKa = 4.28GQSFVYY30 pKa = 10.97VEE32 pKa = 4.74DD33 pKa = 5.33DD34 pKa = 4.33DD35 pKa = 4.77MDD37 pKa = 3.84VLEE40 pKa = 4.84TGEE43 pKa = 4.35CVHH46 pKa = 6.29IQFPGLYY53 pKa = 9.58DD54 pKa = 3.55GQEE57 pKa = 4.05VVFDD61 pKa = 4.01ALVYY65 pKa = 8.56TLRR68 pKa = 11.84LHH70 pKa = 6.7HH71 pKa = 6.86SSLVYY76 pKa = 10.46EE77 pKa = 4.46MAVEE81 pKa = 4.54QIRR84 pKa = 11.84KK85 pKa = 7.25TYY87 pKa = 9.7PEE89 pKa = 4.24YY90 pKa = 10.99VPPEE94 pKa = 4.27DD95 pKa = 4.72RR96 pKa = 11.84PANYY100 pKa = 9.72KK101 pKa = 10.04ISPEE105 pKa = 4.01LEE107 pKa = 4.12EE108 pKa = 4.16EE109 pKa = 4.39AEE111 pKa = 4.22TALTEE116 pKa = 4.51IIDD119 pKa = 4.46EE120 pKa = 4.28IEE122 pKa = 3.82EE123 pKa = 4.43TEE125 pKa = 4.26TVKK128 pKa = 10.73VQEE131 pKa = 4.21HH132 pKa = 5.52VEE134 pKa = 3.95VDD136 pKa = 3.73LEE138 pKa = 4.12ADD140 pKa = 3.33YY141 pKa = 11.32GIALDD146 pKa = 3.4VCLNVEE152 pKa = 4.59EE153 pKa = 4.56ITDD156 pKa = 3.64EE157 pKa = 4.26VIEE160 pKa = 4.15NFIRR164 pKa = 11.84AYY166 pKa = 9.96QSNTLRR172 pKa = 11.84LDD174 pKa = 3.17NTLYY178 pKa = 11.03SFTSEE183 pKa = 4.21SEE185 pKa = 4.1EE186 pKa = 3.98

Molecular weight: 21.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P9WZN5|A0A1P9WZN5_9BACT DNA polymerase III subunit delta OS=Spirosoma montaniterrae OX=1178516 GN=AWR27_16880 PE=4 SV=1
MM1 pKa = 7.69PSSEE5 pKa = 4.14PDD7 pKa = 2.78KK8 pKa = 10.88TRR10 pKa = 11.84PIQRR14 pKa = 11.84NDD16 pKa = 2.57SFLRR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.72DD24 pKa = 2.92KK25 pKa = 11.34LMVYY29 pKa = 10.03VDD31 pKa = 3.17PHH33 pKa = 7.27FGFIRR38 pKa = 11.84QVYY41 pKa = 9.96KK42 pKa = 10.69GVFYY46 pKa = 10.54IKK48 pKa = 10.21KK49 pKa = 9.51CAYY52 pKa = 9.7CKK54 pKa = 9.8RR55 pKa = 11.84TFEE58 pKa = 4.27AKK60 pKa = 9.47RR61 pKa = 11.84RR62 pKa = 11.84HH63 pKa = 5.89AAFCSPTCQQAHH75 pKa = 4.82RR76 pKa = 11.84RR77 pKa = 11.84GRR79 pKa = 11.84NRR81 pKa = 3.12

Molecular weight: 9.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4811

0

4811

1699614

38

3542

353.3

39.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.68 ± 0.038

0.749 ± 0.01

5.415 ± 0.026

5.038 ± 0.035

4.497 ± 0.027

7.366 ± 0.037

1.791 ± 0.017

5.452 ± 0.026

4.325 ± 0.034

10.129 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.078 ± 0.015

4.718 ± 0.038

4.788 ± 0.023

4.488 ± 0.022

6.006 ± 0.029

5.767 ± 0.029

6.582 ± 0.039

7.098 ± 0.026

1.327 ± 0.012

3.706 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski