Spinach amalgavirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; Amalgavirus

Average proteome isoelectric point is 7.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1W6S3T3|A0A1W6S3T3_9VIRU Putative coat protein OS=Spinach amalgavirus 1 OX=1985164 PE=4 SV=1
MM1 pKa = 7.7AGLEE5 pKa = 4.4GEE7 pKa = 4.43GSEE10 pKa = 5.28GIHH13 pKa = 6.25LVEE16 pKa = 4.4KK17 pKa = 10.0TPKK20 pKa = 9.84QEE22 pKa = 3.94QEE24 pKa = 4.13EE25 pKa = 4.9LFTASEE31 pKa = 4.42KK32 pKa = 10.67LRR34 pKa = 11.84EE35 pKa = 4.09AGIPLGAFDD44 pKa = 4.75RR45 pKa = 11.84NAIIKK50 pKa = 10.1AGHH53 pKa = 5.96SFNGYY58 pKa = 8.1MKK60 pKa = 9.83MIKK63 pKa = 10.25YY64 pKa = 6.31VTNYY68 pKa = 9.68TEE70 pKa = 5.41GGFFDD75 pKa = 4.06TLLVMGGSKK84 pKa = 10.31KK85 pKa = 10.51LYY87 pKa = 8.9PIHH90 pKa = 7.34SKK92 pKa = 9.41MDD94 pKa = 3.05HH95 pKa = 6.13FGFVRR100 pKa = 11.84FARR103 pKa = 11.84WLQSKK108 pKa = 9.76EE109 pKa = 4.15GQDD112 pKa = 3.97EE113 pKa = 4.86IYY115 pKa = 10.34TLQRR119 pKa = 11.84EE120 pKa = 4.39QKK122 pKa = 9.29LLRR125 pKa = 11.84KK126 pKa = 9.64AGEE129 pKa = 4.43SLTPTQMIKK138 pKa = 10.71NDD140 pKa = 3.54VFNLIRR146 pKa = 11.84TEE148 pKa = 4.18FSRR151 pKa = 11.84AMKK154 pKa = 10.53AEE156 pKa = 3.91RR157 pKa = 11.84EE158 pKa = 4.1KK159 pKa = 11.18FEE161 pKa = 4.04QEE163 pKa = 3.3KK164 pKa = 11.34DD165 pKa = 3.09EE166 pKa = 4.13LRR168 pKa = 11.84RR169 pKa = 11.84LLRR172 pKa = 11.84QKK174 pKa = 10.43EE175 pKa = 4.12RR176 pKa = 11.84EE177 pKa = 3.67EE178 pKa = 3.77RR179 pKa = 11.84QAFKK183 pKa = 11.09NLQEE187 pKa = 4.12KK188 pKa = 8.96FAPISFYY195 pKa = 10.88RR196 pKa = 11.84EE197 pKa = 3.81PTDD200 pKa = 3.47EE201 pKa = 4.17EE202 pKa = 4.37VGIAAYY208 pKa = 10.04EE209 pKa = 4.09MYY211 pKa = 10.67EE212 pKa = 3.86NEE214 pKa = 3.6ARR216 pKa = 11.84KK217 pKa = 9.86NGKK220 pKa = 7.46TPMSRR225 pKa = 11.84YY226 pKa = 9.72HH227 pKa = 6.97GGDD230 pKa = 2.89VYY232 pKa = 11.34ARR234 pKa = 11.84QHH236 pKa = 6.27FSQAARR242 pKa = 11.84EE243 pKa = 3.93LAQVYY248 pKa = 8.03FASNPEE254 pKa = 3.88NQDD257 pKa = 2.56IMEE260 pKa = 4.59RR261 pKa = 11.84FMKK264 pKa = 10.29EE265 pKa = 2.97RR266 pKa = 11.84FLFFRR271 pKa = 11.84KK272 pKa = 7.71TADD275 pKa = 3.14EE276 pKa = 4.3SGEE279 pKa = 3.98RR280 pKa = 11.84TARR283 pKa = 11.84MQLVGIGGEE292 pKa = 4.18KK293 pKa = 10.21AVEE296 pKa = 3.87DD297 pKa = 4.19ALAEE301 pKa = 4.0EE302 pKa = 4.51AKK304 pKa = 10.62AYY306 pKa = 10.6GDD308 pKa = 4.43DD309 pKa = 4.12PDD311 pKa = 5.2RR312 pKa = 11.84DD313 pKa = 3.93SAGAGAAGEE322 pKa = 4.21VQGASKK328 pKa = 10.22KK329 pKa = 10.01AKK331 pKa = 9.19PRR333 pKa = 11.84FVSKK337 pKa = 10.55PPTGRR342 pKa = 11.84GKK344 pKa = 10.27AAAGDD349 pKa = 3.97QPEE352 pKa = 4.82GGADD356 pKa = 3.44QPGSEE361 pKa = 4.45DD362 pKa = 5.7AEE364 pKa = 4.44DD365 pKa = 3.87QSDD368 pKa = 3.81TSSTLSRR375 pKa = 11.84PRR377 pKa = 11.84KK378 pKa = 6.11TARR381 pKa = 11.84NTRR384 pKa = 11.84SKK386 pKa = 10.8KK387 pKa = 10.2

Molecular weight:
43.69 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1W6S3T3|A0A1W6S3T3_9VIRU Putative coat protein OS=Spinach amalgavirus 1 OX=1985164 PE=4 SV=1
MM1 pKa = 7.7AGLEE5 pKa = 4.4GEE7 pKa = 4.43GSEE10 pKa = 5.28GIHH13 pKa = 6.25LVEE16 pKa = 4.4KK17 pKa = 10.0TPKK20 pKa = 9.84QEE22 pKa = 3.94QEE24 pKa = 4.13EE25 pKa = 4.9LFTASEE31 pKa = 4.42KK32 pKa = 10.67LRR34 pKa = 11.84EE35 pKa = 4.09AGIPLGAFDD44 pKa = 4.75RR45 pKa = 11.84NAIIKK50 pKa = 10.1AGHH53 pKa = 5.96SFNGYY58 pKa = 8.1MKK60 pKa = 9.83MIKK63 pKa = 10.25YY64 pKa = 6.31VTNYY68 pKa = 9.68TEE70 pKa = 5.41GGFFDD75 pKa = 4.06TLLVMGGSKK84 pKa = 10.31KK85 pKa = 10.51LYY87 pKa = 8.9PIHH90 pKa = 7.34SKK92 pKa = 9.41MDD94 pKa = 3.05HH95 pKa = 6.13FGFVRR100 pKa = 11.84FARR103 pKa = 11.84WLQSKK108 pKa = 9.76EE109 pKa = 4.15GQDD112 pKa = 3.97EE113 pKa = 4.86IYY115 pKa = 10.34TLQRR119 pKa = 11.84EE120 pKa = 4.39QKK122 pKa = 9.29LLRR125 pKa = 11.84KK126 pKa = 9.64AGEE129 pKa = 4.43SLTPTQMIKK138 pKa = 10.71NDD140 pKa = 3.54VFNLIRR146 pKa = 11.84TEE148 pKa = 4.18FSRR151 pKa = 11.84AMKK154 pKa = 10.53AEE156 pKa = 3.91RR157 pKa = 11.84EE158 pKa = 4.1KK159 pKa = 11.18FEE161 pKa = 4.04QEE163 pKa = 3.3KK164 pKa = 11.34DD165 pKa = 3.09EE166 pKa = 4.13LRR168 pKa = 11.84RR169 pKa = 11.84LLRR172 pKa = 11.84QKK174 pKa = 10.43EE175 pKa = 4.12RR176 pKa = 11.84EE177 pKa = 3.67EE178 pKa = 3.77RR179 pKa = 11.84QAFKK183 pKa = 11.09NLQEE187 pKa = 4.12KK188 pKa = 8.96FAPISFYY195 pKa = 10.88RR196 pKa = 11.84EE197 pKa = 3.81PTDD200 pKa = 3.47EE201 pKa = 4.17EE202 pKa = 4.37VGIAAYY208 pKa = 10.04EE209 pKa = 4.09MYY211 pKa = 10.67EE212 pKa = 3.86NEE214 pKa = 3.6ARR216 pKa = 11.84KK217 pKa = 9.86NGKK220 pKa = 7.46TPMSRR225 pKa = 11.84YY226 pKa = 9.72HH227 pKa = 6.97GGDD230 pKa = 2.89VYY232 pKa = 11.34ARR234 pKa = 11.84QHH236 pKa = 6.27FSQAARR242 pKa = 11.84EE243 pKa = 3.93LAQVYY248 pKa = 8.03FASNPEE254 pKa = 3.88NQDD257 pKa = 2.56IMEE260 pKa = 4.59RR261 pKa = 11.84FMKK264 pKa = 10.3EE265 pKa = 3.01RR266 pKa = 11.84FLFFGRR272 pKa = 11.84PPMNLGSAQQEE283 pKa = 4.61CSWLALVEE291 pKa = 4.16RR292 pKa = 11.84KK293 pKa = 8.72LWKK296 pKa = 9.69MPLQRR301 pKa = 11.84RR302 pKa = 11.84LRR304 pKa = 11.84LTGMIPIGILQAPGRR319 pKa = 11.84RR320 pKa = 11.84GRR322 pKa = 11.84CRR324 pKa = 11.84EE325 pKa = 3.98LRR327 pKa = 11.84RR328 pKa = 11.84KK329 pKa = 10.17LSPDD333 pKa = 3.26LLASPRR339 pKa = 11.84QVGVKK344 pKa = 9.43LQQGISRR351 pKa = 11.84KK352 pKa = 9.74VEE354 pKa = 3.92LTSLGPKK361 pKa = 8.44MLRR364 pKa = 11.84TSRR367 pKa = 11.84IRR369 pKa = 11.84VLRR372 pKa = 11.84SLGPEE377 pKa = 3.84RR378 pKa = 11.84PRR380 pKa = 11.84AIPVARR386 pKa = 11.84SRR388 pKa = 11.84FEE390 pKa = 3.96AGVRR394 pKa = 11.84KK395 pKa = 10.21VIGGGAMRR403 pKa = 11.84SWEE406 pKa = 4.43VDD408 pKa = 2.97SQMYY412 pKa = 10.23RR413 pKa = 11.84GGGNSADD420 pKa = 3.82ALRR423 pKa = 11.84LLGQARR429 pKa = 11.84DD430 pKa = 3.89DD431 pKa = 3.73RR432 pKa = 11.84PGAFLSGKK440 pKa = 8.03FTQASARR447 pKa = 11.84LALLLPNNLDD457 pKa = 3.5VPDD460 pKa = 4.4GAKK463 pKa = 8.35STRR466 pKa = 11.84MKK468 pKa = 10.89NFNNDD473 pKa = 2.39ATAGPFLRR481 pKa = 11.84SFGIKK486 pKa = 9.75GKK488 pKa = 10.75YY489 pKa = 7.62GLKK492 pKa = 10.15RR493 pKa = 11.84KK494 pKa = 10.1LEE496 pKa = 4.14EE497 pKa = 4.18EE498 pKa = 3.56MWRR501 pKa = 11.84YY502 pKa = 9.62YY503 pKa = 11.08DD504 pKa = 4.59DD505 pKa = 4.21YY506 pKa = 11.34GAGRR510 pKa = 11.84IDD512 pKa = 3.73SSGLPFFTARR522 pKa = 11.84VGFRR526 pKa = 11.84TKK528 pKa = 10.47LVSVEE533 pKa = 3.76KK534 pKa = 10.93AEE536 pKa = 4.48EE537 pKa = 4.14KK538 pKa = 10.74FKK540 pKa = 10.52TGQPFGRR547 pKa = 11.84AVMMLDD553 pKa = 3.64ALEE556 pKa = 4.0QAAASPLYY564 pKa = 10.4NVLSHH569 pKa = 5.37YY570 pKa = 9.11TFEE573 pKa = 4.01RR574 pKa = 11.84RR575 pKa = 11.84LRR577 pKa = 11.84RR578 pKa = 11.84DD579 pKa = 3.25CGFKK583 pKa = 10.55NAVIRR588 pKa = 11.84ASSDD592 pKa = 2.65WNEE595 pKa = 3.1IWKK598 pKa = 9.95GVRR601 pKa = 11.84EE602 pKa = 4.11AEE604 pKa = 5.06AIIEE608 pKa = 4.16LDD610 pKa = 2.75WSKK613 pKa = 10.98FDD615 pKa = 3.97RR616 pKa = 11.84EE617 pKa = 4.2RR618 pKa = 11.84PAEE621 pKa = 4.18DD622 pKa = 3.94LEE624 pKa = 4.88FIVDD628 pKa = 3.7VVISCFKK635 pKa = 10.07PRR637 pKa = 11.84NEE639 pKa = 3.86RR640 pKa = 11.84EE641 pKa = 3.75RR642 pKa = 11.84RR643 pKa = 11.84LLRR646 pKa = 11.84AYY648 pKa = 10.34GIMMRR653 pKa = 11.84RR654 pKa = 11.84ALIEE658 pKa = 4.04RR659 pKa = 11.84LLVMDD664 pKa = 4.42GGGVFGIEE672 pKa = 3.97GMVPSGSLWTGWIDD686 pKa = 3.17TALNVLYY693 pKa = 10.56LRR695 pKa = 11.84SACLEE700 pKa = 3.91VGLPSQRR707 pKa = 11.84YY708 pKa = 8.54LPMCAGDD715 pKa = 4.88DD716 pKa = 3.76NLTLFWSDD724 pKa = 3.06PGEE727 pKa = 4.2VKK729 pKa = 10.5LLKK732 pKa = 10.05IRR734 pKa = 11.84EE735 pKa = 4.45LLNEE739 pKa = 4.6WYY741 pKa = 10.28RR742 pKa = 11.84AGIDD746 pKa = 3.45DD747 pKa = 4.78ADD749 pKa = 4.24FFIHH753 pKa = 6.76RR754 pKa = 11.84PPYY757 pKa = 10.21HH758 pKa = 5.19VVKK761 pKa = 10.43RR762 pKa = 11.84QACFPPGTDD771 pKa = 3.32LKK773 pKa = 10.97GGTSMLMKK781 pKa = 10.1DD782 pKa = 3.8AEE784 pKa = 4.23WVEE787 pKa = 4.25FEE789 pKa = 4.08GEE791 pKa = 4.16LRR793 pKa = 11.84VDD795 pKa = 3.79EE796 pKa = 4.49AAGRR800 pKa = 11.84SHH802 pKa = 7.02RR803 pKa = 11.84WQYY806 pKa = 10.95LFKK809 pKa = 10.77GRR811 pKa = 11.84PKK813 pKa = 10.46FLSCYY818 pKa = 4.9WTRR821 pKa = 11.84DD822 pKa = 3.37GLPIRR827 pKa = 11.84PTSDD831 pKa = 3.16NLEE834 pKa = 4.07KK835 pKa = 10.94LLWPEE840 pKa = 5.26GIHH843 pKa = 7.21KK844 pKa = 10.88SIDD847 pKa = 3.44DD848 pKa = 3.97YY849 pKa = 10.88EE850 pKa = 5.84ASVASMAVDD859 pKa = 3.42NPFNHH864 pKa = 6.72HH865 pKa = 6.04NVNHH869 pKa = 5.88MLMRR873 pKa = 11.84YY874 pKa = 9.69VIIQQIRR881 pKa = 11.84RR882 pKa = 11.84VSAGILSPEE891 pKa = 3.98EE892 pKa = 3.81CLAFCKK898 pKa = 10.2FRR900 pKa = 11.84SQEE903 pKa = 4.08GEE905 pKa = 4.12EE906 pKa = 4.03IPYY909 pKa = 10.62PMVAPWRR916 pKa = 11.84RR917 pKa = 11.84GMHH920 pKa = 5.5EE921 pKa = 4.78ARR923 pKa = 11.84MEE925 pKa = 4.71DD926 pKa = 3.84YY927 pKa = 10.88PEE929 pKa = 4.01TKK931 pKa = 9.62PWVKK935 pKa = 10.21EE936 pKa = 3.62FRR938 pKa = 11.84DD939 pKa = 4.82FISGVSSLYY948 pKa = 10.43ARR950 pKa = 11.84KK951 pKa = 8.27PTGGVDD957 pKa = 3.51AYY959 pKa = 11.16RR960 pKa = 11.84FMEE963 pKa = 4.95IIRR966 pKa = 11.84GEE968 pKa = 3.96QSPGEE973 pKa = 4.12GQFGSDD979 pKa = 3.12LVKK982 pKa = 10.35WVDD985 pKa = 3.39WLRR988 pKa = 11.84KK989 pKa = 9.53HH990 pKa = 6.28PVTKK994 pKa = 10.56FLRR997 pKa = 11.84ATGGRR1002 pKa = 11.84HH1003 pKa = 4.68HH1004 pKa = 6.68QKK1006 pKa = 8.83STARR1010 pKa = 11.84LEE1012 pKa = 4.57GEE1014 pKa = 4.39DD1015 pKa = 3.6LRR1017 pKa = 11.84KK1018 pKa = 10.37VEE1020 pKa = 4.4DD1021 pKa = 3.21AFMALRR1027 pKa = 11.84EE1028 pKa = 4.14RR1029 pKa = 11.84LGSGQVGSTEE1039 pKa = 5.05DD1040 pKa = 3.47FSLWVSDD1047 pKa = 3.9LLRR1050 pKa = 11.84SGMM1053 pKa = 4.04

Molecular weight:
120.85 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1440

387

1053

720.0

82.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.917 ± 1.141

0.694 ± 0.367

5.0 ± 0.089

9.236 ± 1.126

5.208 ± 0.115

8.542 ± 0.129

1.667 ± 0.061

4.028 ± 0.353

6.597 ± 1.019

8.542 ± 1.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.403 ± 0.159

2.778 ± 0.034

4.514 ± 0.337

3.958 ± 0.639

9.375 ± 0.72

5.903 ± 0.115

3.611 ± 0.549

4.514 ± 0.746

1.528 ± 0.67

2.986 ± 0.061

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski