Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi s sarcoma-associated herpesvirus)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Gammaherpesvirinae; Rhadinovirus; Human gammaherpesvirus 8

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q2HRB6-2|SCAF-2_HHV8P Isoform of Q2HRB6 Isoform pAP of Capsid scaffolding protein OS=Human herpesvirus 8 type P (isolate GK18) OX=868565 GN=ORF17 PE=1 SV=1
MM1 pKa = 7.75APPGMRR7 pKa = 11.84LRR9 pKa = 11.84SGRR12 pKa = 11.84STGAPLTRR20 pKa = 11.84GSCRR24 pKa = 11.84KK25 pKa = 9.62RR26 pKa = 11.84NRR28 pKa = 11.84SPEE31 pKa = 3.97RR32 pKa = 11.84CDD34 pKa = 5.09LGDD37 pKa = 4.86DD38 pKa = 3.78LHH40 pKa = 6.35LQPRR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 9.5HH47 pKa = 5.62VADD50 pKa = 3.58SVDD53 pKa = 3.17GRR55 pKa = 11.84EE56 pKa = 4.62CGPHH60 pKa = 5.99TLPIPGSPTVFTSGLPAFVSSPTLPVAPIPSPAPATPLPPPALLPPVTTSSSPIPPSHH118 pKa = 6.71PVSPGTTDD126 pKa = 2.91THH128 pKa = 6.78SPSPALPPTQSPEE141 pKa = 3.81SSQRR145 pKa = 11.84PPLSSPTGRR154 pKa = 11.84PDD156 pKa = 2.63SSTPMRR162 pKa = 11.84PPPSQQTTPPHH173 pKa = 6.63SPTTPPPEE181 pKa = 4.72PPSKK185 pKa = 10.64SSPDD189 pKa = 3.28SLAPSTLRR197 pKa = 11.84SLRR200 pKa = 11.84KK201 pKa = 9.2RR202 pKa = 11.84RR203 pKa = 11.84LSSPQGPSTLNPICQSPPVSPPRR226 pKa = 11.84CDD228 pKa = 3.09FANRR232 pKa = 11.84SVYY235 pKa = 10.06PPWATEE241 pKa = 3.69SPIYY245 pKa = 10.41VGSSSDD251 pKa = 3.01GDD253 pKa = 3.85TPPRR257 pKa = 11.84QPPTSPISIGSSSPSEE273 pKa = 4.18GSWGDD278 pKa = 3.52DD279 pKa = 2.79TAMLVLLAEE288 pKa = 4.45IAEE291 pKa = 4.14EE292 pKa = 3.7ASKK295 pKa = 11.3NEE297 pKa = 4.25KK298 pKa = 9.88EE299 pKa = 4.32CSEE302 pKa = 4.02NNQAGEE308 pKa = 4.28DD309 pKa = 3.65NGDD312 pKa = 3.55NEE314 pKa = 4.33ISKK317 pKa = 10.3EE318 pKa = 4.18SQVDD322 pKa = 3.67KK323 pKa = 11.19DD324 pKa = 4.68DD325 pKa = 4.5NDD327 pKa = 3.72NKK329 pKa = 10.87DD330 pKa = 3.69DD331 pKa = 4.22EE332 pKa = 4.92EE333 pKa = 4.21EE334 pKa = 4.3QEE336 pKa = 3.97TDD338 pKa = 4.05EE339 pKa = 4.96EE340 pKa = 4.57DD341 pKa = 3.94EE342 pKa = 4.7EE343 pKa = 6.24DD344 pKa = 5.14DD345 pKa = 5.21EE346 pKa = 6.18EE347 pKa = 6.72DD348 pKa = 5.0DD349 pKa = 5.25EE350 pKa = 6.16EE351 pKa = 6.7DD352 pKa = 5.0DD353 pKa = 5.25EE354 pKa = 6.16EE355 pKa = 6.7DD356 pKa = 5.0DD357 pKa = 5.25EE358 pKa = 6.16EE359 pKa = 6.7DD360 pKa = 5.0DD361 pKa = 5.25EE362 pKa = 6.16EE363 pKa = 6.7DD364 pKa = 5.0DD365 pKa = 4.88EE366 pKa = 6.06EE367 pKa = 6.43DD368 pKa = 5.02DD369 pKa = 4.1EE370 pKa = 5.75EE371 pKa = 4.55EE372 pKa = 5.15DD373 pKa = 3.76EE374 pKa = 5.3EE375 pKa = 4.38EE376 pKa = 5.36DD377 pKa = 3.77EE378 pKa = 5.34EE379 pKa = 4.41EE380 pKa = 5.36DD381 pKa = 3.77EE382 pKa = 5.16EE383 pKa = 4.34EE384 pKa = 4.89DD385 pKa = 3.92EE386 pKa = 4.92EE387 pKa = 4.62EE388 pKa = 4.97EE389 pKa = 4.72EE390 pKa = 5.79DD391 pKa = 5.51EE392 pKa = 5.53EE393 pKa = 6.97DD394 pKa = 6.31DD395 pKa = 5.53DD396 pKa = 7.12DD397 pKa = 5.49EE398 pKa = 6.88DD399 pKa = 5.88NEE401 pKa = 4.97DD402 pKa = 3.85EE403 pKa = 4.7EE404 pKa = 6.42DD405 pKa = 5.51DD406 pKa = 3.92EE407 pKa = 5.88EE408 pKa = 4.28EE409 pKa = 4.41DD410 pKa = 4.01KK411 pKa = 11.62KK412 pKa = 11.23EE413 pKa = 4.03DD414 pKa = 3.96EE415 pKa = 4.45EE416 pKa = 6.55DD417 pKa = 3.72GGDD420 pKa = 3.74GNKK423 pKa = 8.77TLSIQSSQQQQEE435 pKa = 4.2PQQQEE440 pKa = 4.09PQQQEE445 pKa = 4.09PQQQEE450 pKa = 4.09PQQQEE455 pKa = 4.09PQQQEE460 pKa = 4.09PQQQEE465 pKa = 4.09PQQQEE470 pKa = 3.9PQQRR474 pKa = 11.84EE475 pKa = 3.91PQQRR479 pKa = 11.84EE480 pKa = 3.78PQQRR484 pKa = 11.84EE485 pKa = 3.78PQQRR489 pKa = 11.84EE490 pKa = 3.78PQQRR494 pKa = 11.84EE495 pKa = 3.78PQQRR499 pKa = 11.84EE500 pKa = 3.78PQQRR504 pKa = 11.84EE505 pKa = 3.78PQQRR509 pKa = 11.84EE510 pKa = 3.78PQQRR514 pKa = 11.84EE515 pKa = 3.78PQQRR519 pKa = 11.84EE520 pKa = 3.78PQQRR524 pKa = 11.84EE525 pKa = 3.78PQQRR529 pKa = 11.84EE530 pKa = 3.94PQQQEE535 pKa = 3.96PQQQEE540 pKa = 4.09PQQQEE545 pKa = 4.09PQQQEE550 pKa = 4.09PQQQEE555 pKa = 4.09PQQQEE560 pKa = 4.09PQQQEE565 pKa = 4.09PQQQEE570 pKa = 4.09PQQQEE575 pKa = 4.09PQQQEE580 pKa = 4.09PQQQEE585 pKa = 4.09PQQQEE590 pKa = 3.99PQQQDD595 pKa = 3.2EE596 pKa = 4.35QQQDD600 pKa = 3.38EE601 pKa = 4.34QQQDD605 pKa = 3.38EE606 pKa = 4.34QQQDD610 pKa = 3.38EE611 pKa = 4.34QQQDD615 pKa = 3.38EE616 pKa = 4.34QQQDD620 pKa = 3.38EE621 pKa = 4.34QQQDD625 pKa = 3.38EE626 pKa = 4.37QQQDD630 pKa = 3.42EE631 pKa = 4.55QEE633 pKa = 4.31QQDD636 pKa = 3.86EE637 pKa = 4.34QQQDD641 pKa = 3.34EE642 pKa = 4.35QQQQDD647 pKa = 3.49EE648 pKa = 4.51QEE650 pKa = 4.15QQEE653 pKa = 4.3EE654 pKa = 4.39QEE656 pKa = 4.26QQEE659 pKa = 4.41EE660 pKa = 4.29QQQDD664 pKa = 3.44EE665 pKa = 4.39QQQDD669 pKa = 3.38EE670 pKa = 4.34QQQDD674 pKa = 3.38EE675 pKa = 4.37QQQDD679 pKa = 3.42EE680 pKa = 4.55QEE682 pKa = 4.31QQDD685 pKa = 3.86EE686 pKa = 4.34QQQDD690 pKa = 3.34EE691 pKa = 4.35QQQQDD696 pKa = 3.49EE697 pKa = 4.51QEE699 pKa = 4.15QQEE702 pKa = 4.3EE703 pKa = 4.39QEE705 pKa = 4.16QQEE708 pKa = 4.29EE709 pKa = 4.38QEE711 pKa = 4.16QQEE714 pKa = 4.29EE715 pKa = 4.38QEE717 pKa = 4.16QQEE720 pKa = 4.35EE721 pKa = 4.41QEE723 pKa = 4.38QEE725 pKa = 4.17LEE727 pKa = 4.15EE728 pKa = 4.07QEE730 pKa = 4.42QEE732 pKa = 4.69LEE734 pKa = 4.15EE735 pKa = 4.07QEE737 pKa = 4.42QEE739 pKa = 4.69LEE741 pKa = 4.15EE742 pKa = 4.07QEE744 pKa = 4.42QEE746 pKa = 4.69LEE748 pKa = 4.15EE749 pKa = 4.07QEE751 pKa = 4.42QEE753 pKa = 4.69LEE755 pKa = 4.15EE756 pKa = 4.07QEE758 pKa = 4.42QEE760 pKa = 4.69LEE762 pKa = 4.15EE763 pKa = 4.07QEE765 pKa = 4.42QEE767 pKa = 4.69LEE769 pKa = 4.15EE770 pKa = 4.07QEE772 pKa = 4.42QEE774 pKa = 4.69LEE776 pKa = 4.15EE777 pKa = 4.07QEE779 pKa = 4.42QEE781 pKa = 4.69LEE783 pKa = 4.15EE784 pKa = 4.07QEE786 pKa = 4.42QEE788 pKa = 4.69LEE790 pKa = 4.15EE791 pKa = 4.07QEE793 pKa = 4.42QEE795 pKa = 4.69LEE797 pKa = 4.15EE798 pKa = 4.07QEE800 pKa = 4.42QEE802 pKa = 4.69LEE804 pKa = 4.15EE805 pKa = 4.07QEE807 pKa = 4.42QEE809 pKa = 4.69LEE811 pKa = 4.15EE812 pKa = 4.07QEE814 pKa = 4.42QEE816 pKa = 4.69LEE818 pKa = 4.17EE819 pKa = 4.1QEE821 pKa = 4.24QEE823 pKa = 4.08QEE825 pKa = 4.07LEE827 pKa = 4.06EE828 pKa = 4.42VEE830 pKa = 4.38EE831 pKa = 4.22QEE833 pKa = 4.93QEE835 pKa = 4.04QEE837 pKa = 4.12EE838 pKa = 4.52QEE840 pKa = 4.18LEE842 pKa = 4.06EE843 pKa = 4.61VEE845 pKa = 4.38EE846 pKa = 4.22QEE848 pKa = 4.93QEE850 pKa = 3.97QEE852 pKa = 4.09EE853 pKa = 4.39QEE855 pKa = 4.2EE856 pKa = 4.48QEE858 pKa = 4.15LEE860 pKa = 4.05EE861 pKa = 4.34VEE863 pKa = 4.21EE864 pKa = 4.21QEE866 pKa = 4.18EE867 pKa = 4.43QEE869 pKa = 4.13LEE871 pKa = 4.05EE872 pKa = 4.34VEE874 pKa = 4.21EE875 pKa = 4.21QEE877 pKa = 4.18EE878 pKa = 4.43QEE880 pKa = 4.13LEE882 pKa = 4.06EE883 pKa = 4.74VEE885 pKa = 4.41EE886 pKa = 4.22QEE888 pKa = 4.27QQGVEE893 pKa = 4.0QQEE896 pKa = 4.31QEE898 pKa = 4.47TVEE901 pKa = 4.17EE902 pKa = 4.66PIILHH907 pKa = 6.37GSSSEE912 pKa = 4.27DD913 pKa = 3.39EE914 pKa = 4.5MEE916 pKa = 3.4VDD918 pKa = 3.84YY919 pKa = 10.89PVVSTHH925 pKa = 5.49EE926 pKa = 4.56QIASSPPGDD935 pKa = 3.92NTPDD939 pKa = 5.1DD940 pKa = 4.93DD941 pKa = 4.49PQPGPSRR948 pKa = 11.84EE949 pKa = 3.78YY950 pKa = 10.3RR951 pKa = 11.84YY952 pKa = 10.66VLRR955 pKa = 11.84TSPPHH960 pKa = 5.87RR961 pKa = 11.84PGVRR965 pKa = 11.84MRR967 pKa = 11.84RR968 pKa = 11.84VPVTHH973 pKa = 7.12PKK975 pKa = 10.05KK976 pKa = 9.84PHH978 pKa = 6.45PRR980 pKa = 11.84YY981 pKa = 8.97QQPPVPYY988 pKa = 9.64RR989 pKa = 11.84QIDD992 pKa = 3.68DD993 pKa = 4.36CPAKK997 pKa = 10.46ARR999 pKa = 11.84PQHH1002 pKa = 4.69IFYY1005 pKa = 10.7RR1006 pKa = 11.84RR1007 pKa = 11.84FLGKK1011 pKa = 10.01DD1012 pKa = 2.94GRR1014 pKa = 11.84RR1015 pKa = 11.84DD1016 pKa = 3.68PKK1018 pKa = 10.69CQWKK1022 pKa = 9.05FAVIFWGNDD1031 pKa = 2.98PYY1033 pKa = 11.57GLKK1036 pKa = 10.41KK1037 pKa = 10.79LSQAFQFGGVKK1048 pKa = 10.3AGPVSCLPHH1057 pKa = 6.94PGPDD1061 pKa = 3.33QSPITYY1067 pKa = 9.65CVYY1070 pKa = 10.48VYY1072 pKa = 10.17CQNKK1076 pKa = 8.21DD1077 pKa = 3.17TSKK1080 pKa = 10.09KK1081 pKa = 9.13VQMARR1086 pKa = 11.84LAWEE1090 pKa = 4.34ASHH1093 pKa = 7.2PLAGNLQSSIVKK1105 pKa = 9.82FKK1107 pKa = 11.13KK1108 pKa = 9.77PLPLTQPGEE1117 pKa = 4.15NQGPGDD1123 pKa = 4.34SPQEE1127 pKa = 3.99MTT1129 pKa = 4.75

Molecular weight: 131.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q2HRB3|CVC2_HHV8P Capsid vertex component 2 OS=Human herpesvirus 8 type P (isolate GK18) OX=868565 GN=CVC2 PE=1 SV=1
MM1 pKa = 7.09VRR3 pKa = 11.84PTEE6 pKa = 4.32AEE8 pKa = 4.15VKK10 pKa = 10.25KK11 pKa = 10.92SLSRR15 pKa = 11.84LPAARR20 pKa = 11.84KK21 pKa = 8.57RR22 pKa = 11.84AGNRR26 pKa = 11.84AHH28 pKa = 7.16LATYY32 pKa = 10.23RR33 pKa = 11.84RR34 pKa = 11.84LLKK37 pKa = 10.9YY38 pKa = 9.62STLPDD43 pKa = 3.55LWRR46 pKa = 11.84FLSSRR51 pKa = 11.84PQNPPLGHH59 pKa = 6.4HH60 pKa = 6.32RR61 pKa = 11.84LFFEE65 pKa = 4.05VTLGHH70 pKa = 7.24RR71 pKa = 11.84IADD74 pKa = 3.86CVILVSGGHH83 pKa = 5.17QPVCYY88 pKa = 9.98VVEE91 pKa = 4.48LKK93 pKa = 9.66TCLSHH98 pKa = 7.52QLIPTNTVRR107 pKa = 11.84TSQRR111 pKa = 11.84AQGLCQLSDD120 pKa = 3.95SIHH123 pKa = 6.49YY124 pKa = 8.93IAHH127 pKa = 6.19SAPPGTEE134 pKa = 3.13AWTITPLLIFKK145 pKa = 9.1NQKK148 pKa = 5.9TLKK151 pKa = 8.46TVYY154 pKa = 10.02SEE156 pKa = 4.51SPGAFPTPVHH166 pKa = 4.73TTEE169 pKa = 4.61GKK171 pKa = 10.16LCAFLTARR179 pKa = 11.84EE180 pKa = 3.97NADD183 pKa = 2.87IRR185 pKa = 11.84KK186 pKa = 9.29VLSKK190 pKa = 10.84VPKK193 pKa = 9.01KK194 pKa = 10.53PKK196 pKa = 8.73MDD198 pKa = 3.54RR199 pKa = 11.84GGKK202 pKa = 9.2ILGPTPGKK210 pKa = 9.9RR211 pKa = 11.84AVYY214 pKa = 9.79SQAHH218 pKa = 6.28HH219 pKa = 6.17GRR221 pKa = 11.84NKK223 pKa = 10.25KK224 pKa = 8.96GRR226 pKa = 11.84PWTAQPTRR234 pKa = 11.84AKK236 pKa = 10.67SRR238 pKa = 11.84TKK240 pKa = 10.97DD241 pKa = 2.83KK242 pKa = 9.8GTPAFPRR249 pKa = 11.84AGPACSGPP257 pKa = 3.49

Molecular weight: 28.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

1

86

38742

60

2635

450.5

50.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.591 ± 0.289

2.548 ± 0.189

4.685 ± 0.177

5.568 ± 0.491

4.161 ± 0.192

6.161 ± 0.222

2.746 ± 0.102

4.535 ± 0.164

3.761 ± 0.16

10.374 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.049 ± 0.113

3.221 ± 0.149

6.967 ± 0.352

4.455 ± 0.535

5.849 ± 0.181

7.591 ± 0.22

6.582 ± 0.202

6.961 ± 0.23

1.223 ± 0.077

2.971 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski