Anaplasma phagocytophilum (strain HZ)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Anaplasmataceae; Anaplasma; phagocytophilum group; Anaplasma phagocytophilum

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1330 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2GL18|Q2GL18_ANAPZ Replicative DNA helicase OS=Anaplasma phagocytophilum (strain HZ) OX=212042 GN=dnaB PE=3 SV=1
MM1 pKa = 7.41SSCFGYY7 pKa = 10.66LPSKK11 pKa = 10.74VLGAITATQIFSYY24 pKa = 10.73IDD26 pKa = 3.61DD27 pKa = 4.33PKK29 pKa = 11.33VAVLEE34 pKa = 4.61NIEE37 pKa = 4.46SGDD40 pKa = 3.99FSGLTAWLSPRR51 pKa = 11.84SLFSAFFLVIASEE64 pKa = 4.22YY65 pKa = 9.72QALSSYY71 pKa = 9.65TCADD75 pKa = 3.5LASRR79 pKa = 11.84SLEE82 pKa = 3.82VDD84 pKa = 3.32ALAVSPVTALLVVVFLISTGFSTTSVEE111 pKa = 4.58FTASS115 pKa = 3.04

Molecular weight: 12.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2GL32|Q2GL32_ANAPZ Sodium:alanine symporter family protein OS=Anaplasma phagocytophilum (strain HZ) OX=212042 GN=APH_0307 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84IVRR12 pKa = 11.84KK13 pKa = 9.26RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 8.59WGRR28 pKa = 11.84RR29 pKa = 11.84ILNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84SILCAA44 pKa = 3.72

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1330

0

1330

347830

30

3373

261.5

28.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.909 ± 0.066

1.861 ± 0.039

5.344 ± 0.061

6.206 ± 0.079

4.04 ± 0.057

7.15 ± 0.096

2.203 ± 0.039

6.691 ± 0.068

5.865 ± 0.054

9.518 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.036

3.926 ± 0.045

3.284 ± 0.047

2.762 ± 0.045

5.064 ± 0.058

8.338 ± 0.07

4.833 ± 0.061

8.298 ± 0.089

0.745 ± 0.021

3.332 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski