Mycobacterium phage Pinnie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482JC04|A0A482JC04_9CAUD Uncharacterized protein OS=Mycobacterium phage Pinnie OX=2517965 GN=54 PE=4 SV=1
MM1 pKa = 7.49ALIEE5 pKa = 5.43DD6 pKa = 4.3MPEE9 pKa = 4.23DD10 pKa = 4.32LPTSNALDD18 pKa = 4.36DD19 pKa = 4.63VDD21 pKa = 6.15DD22 pKa = 4.96PDD24 pKa = 3.53EE25 pKa = 4.48QEE27 pKa = 4.05EE28 pKa = 4.16HH29 pKa = 6.67GEE31 pKa = 3.82RR32 pKa = 11.84AEE34 pKa = 4.12IFLSGAHH41 pKa = 6.0VCTLDD46 pKa = 3.3NAPRR50 pKa = 11.84GLGRR54 pKa = 11.84VTLMVEE60 pKa = 4.17LEE62 pKa = 4.28VTEE65 pKa = 3.99EE66 pKa = 3.85ATRR69 pKa = 11.84FTEE72 pKa = 5.39DD73 pKa = 2.99GTEE76 pKa = 4.01VPIRR80 pKa = 11.84RR81 pKa = 11.84CRR83 pKa = 11.84RR84 pKa = 11.84IGDD87 pKa = 3.17MWIPGTPKK95 pKa = 10.01PPTRR99 pKa = 11.84EE100 pKa = 3.97EE101 pKa = 3.82IKK103 pKa = 11.14AMADD107 pKa = 3.04AAKK110 pKa = 10.48AKK112 pKa = 10.39AQAEE116 pKa = 4.03QLAAEE121 pKa = 4.23RR122 pKa = 11.84AEE124 pKa = 4.08QEE126 pKa = 4.26EE127 pKa = 4.48NEE129 pKa = 4.24PPMFDD134 pKa = 3.47EE135 pKa = 5.75DD136 pKa = 4.41GNPLSEE142 pKa = 5.13DD143 pKa = 3.41APAPADD149 pKa = 4.96DD150 pKa = 4.78EE151 pKa = 5.0DD152 pKa = 3.88QAGDD156 pKa = 3.65GSGDD160 pKa = 3.74ADD162 pKa = 3.88DD163 pKa = 4.71VDD165 pKa = 4.14PAEE168 pKa = 4.71SLGEE172 pKa = 4.06VNPQFSDD179 pKa = 3.5GATT182 pKa = 3.14

Molecular weight: 19.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482JDG3|A0A482JDG3_9CAUD Uncharacterized protein OS=Mycobacterium phage Pinnie OX=2517965 GN=55 PE=4 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84PLYY5 pKa = 10.36VRR7 pKa = 11.84QSEE10 pKa = 4.37AGPNASNTGDD20 pKa = 3.05YY21 pKa = 9.62WYY23 pKa = 9.41TVSTGNGEE31 pKa = 4.69PILTSKK37 pKa = 10.07MYY39 pKa = 8.87RR40 pKa = 11.84TRR42 pKa = 11.84ARR44 pKa = 11.84AIRR47 pKa = 11.84AARR50 pKa = 11.84AFMRR54 pKa = 11.84RR55 pKa = 11.84TRR57 pKa = 11.84GPVTFSYY64 pKa = 9.34WAGYY68 pKa = 8.29TPTQQAEE75 pKa = 3.84RR76 pKa = 11.84MAIGRR81 pKa = 11.84QPRR84 pKa = 11.84GQLQLVTEE92 pKa = 5.29HH93 pKa = 6.19YY94 pKa = 10.71QFADD98 pKa = 3.06

Molecular weight: 11.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

14508

47

1340

219.8

23.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.138 ± 0.623

1.034 ± 0.18

7.203 ± 0.345

5.652 ± 0.471

3.026 ± 0.199

9.422 ± 0.805

2.013 ± 0.198

4.184 ± 0.221

3.426 ± 0.228

7.396 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.082 ± 0.176

3.164 ± 0.184

6.086 ± 0.405

3.453 ± 0.174

7.217 ± 0.475

4.487 ± 0.29

6.252 ± 0.263

6.576 ± 0.244

1.985 ± 0.14

2.206 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski