Phlebovirus GGP-2011a

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; unclassified Phlebovirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1T371|I1T371_9VIRU Nucleoprotein OS=Phlebovirus GGP-2011a OX=1048857 PE=3 SV=1
MM1 pKa = 7.11NRR3 pKa = 11.84FMRR6 pKa = 11.84DD7 pKa = 3.02MPLVGTTGLSLNRR20 pKa = 11.84IQVTYY25 pKa = 10.75IPFNKK30 pKa = 8.47TWNSPVSTYY39 pKa = 11.51DD40 pKa = 3.19VMEE43 pKa = 4.91FPVHH47 pKa = 6.91NYY49 pKa = 7.1TQANEE54 pKa = 3.96IRR56 pKa = 11.84DD57 pKa = 3.99NLQSFYY63 pKa = 11.08RR64 pKa = 11.84LNRR67 pKa = 11.84IPARR71 pKa = 11.84WGPGFPSIRR80 pKa = 11.84NEE82 pKa = 3.54SDD84 pKa = 3.03IEE86 pKa = 4.16HH87 pKa = 7.05LSLIHH92 pKa = 6.34EE93 pKa = 4.64LSKK96 pKa = 10.25IDD98 pKa = 3.83EE99 pKa = 4.38IEE101 pKa = 3.96IVRR104 pKa = 11.84HH105 pKa = 5.31NEE107 pKa = 3.78PNLKK111 pKa = 9.96RR112 pKa = 11.84ALYY115 pKa = 9.56WPFNYY120 pKa = 9.85PSMSFIRR127 pKa = 11.84HH128 pKa = 5.93ASRR131 pKa = 11.84VDD133 pKa = 3.58EE134 pKa = 4.41EE135 pKa = 4.71KK136 pKa = 10.51PWLFKK141 pKa = 11.55NNVATDD147 pKa = 3.4ILRR150 pKa = 11.84ASKK153 pKa = 9.28STVLDD158 pKa = 3.7LAIVNLHH165 pKa = 6.13RR166 pKa = 11.84EE167 pKa = 4.33VLRR170 pKa = 11.84EE171 pKa = 3.98AARR174 pKa = 11.84QSLPEE179 pKa = 4.03GKK181 pKa = 10.4FPGKK185 pKa = 10.19DD186 pKa = 2.65ILMEE190 pKa = 4.22IASLQCRR197 pKa = 11.84RR198 pKa = 11.84MLSAIDD204 pKa = 3.9SDD206 pKa = 4.25SEE208 pKa = 4.21YY209 pKa = 10.81DD210 pKa = 3.96GPHH213 pKa = 5.83SPIFNAVWDD222 pKa = 3.99YY223 pKa = 11.58RR224 pKa = 11.84EE225 pKa = 4.21SCLEE229 pKa = 3.97TVFFSDD235 pKa = 3.39WVPSVDD241 pKa = 5.47EE242 pKa = 4.14FDD244 pKa = 4.1CPIMHH249 pKa = 7.04MMRR252 pKa = 11.84NGLRR256 pKa = 11.84TWDD259 pKa = 3.96PEE261 pKa = 4.14DD262 pKa = 3.87TEE264 pKa = 4.82PEE266 pKa = 3.54VDD268 pKa = 3.15AASGLKK274 pKa = 10.66ANN276 pKa = 4.61

Molecular weight: 32.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1T371|I1T371_9VIRU Nucleoprotein OS=Phlebovirus GGP-2011a OX=1048857 PE=3 SV=1
MM1 pKa = 7.69SDD3 pKa = 3.5YY4 pKa = 11.17EE5 pKa = 4.26RR6 pKa = 11.84IAVEE10 pKa = 4.36FAADD14 pKa = 3.3IPSNEE19 pKa = 4.47VIQEE23 pKa = 3.94WVEE26 pKa = 3.67AFAYY30 pKa = 10.22QGFDD34 pKa = 2.98AAHH37 pKa = 5.18VLKK40 pKa = 10.75RR41 pKa = 11.84LIEE44 pKa = 4.42CGGNNWKK51 pKa = 10.22EE52 pKa = 3.95DD53 pKa = 3.49AKK55 pKa = 10.97KK56 pKa = 10.71LIVIAITRR64 pKa = 11.84GNKK67 pKa = 8.4PDD69 pKa = 4.19KK70 pKa = 10.82IMEE73 pKa = 4.34KK74 pKa = 9.73MSEE77 pKa = 3.97EE78 pKa = 3.9GKK80 pKa = 10.61KK81 pKa = 10.39AFKK84 pKa = 10.29VLKK87 pKa = 10.15DD88 pKa = 3.57RR89 pKa = 11.84YY90 pKa = 9.17RR91 pKa = 11.84LQGGNPGRR99 pKa = 11.84SDD101 pKa = 3.34LTLSRR106 pKa = 11.84IATALAGWTCQACVVVGEE124 pKa = 4.3YY125 pKa = 10.97LPVTIGTMRR134 pKa = 11.84SISDD138 pKa = 3.76NYY140 pKa = 8.69PGAMMHH146 pKa = 6.69PCFAGLIDD154 pKa = 4.33RR155 pKa = 11.84SLPEE159 pKa = 3.96DD160 pKa = 3.52TKK162 pKa = 11.43LVIINAFCLFMVHH175 pKa = 5.56FTKK178 pKa = 10.79VINPRR183 pKa = 11.84MRR185 pKa = 11.84SSPVSEE191 pKa = 4.12VVNSFRR197 pKa = 11.84QPMLAAINSNFLNSDD212 pKa = 2.98QRR214 pKa = 11.84KK215 pKa = 9.16GFLKK219 pKa = 10.83SLGILDD225 pKa = 5.53ANLQPTAPVRR235 pKa = 11.84AAARR239 pKa = 11.84AYY241 pKa = 9.97EE242 pKa = 4.06LLAA245 pKa = 5.59

Molecular weight: 27.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3958

245

2098

989.5

111.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.533 ± 0.594

2.602 ± 0.684

5.533 ± 0.352

6.948 ± 0.47

4.396 ± 0.473

5.659 ± 0.359

2.602 ± 0.298

6.721 ± 0.108

6.139 ± 0.408

8.489 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.234 ± 0.369

4.144 ± 0.534

4.295 ± 0.365

2.83 ± 0.128

5.76 ± 0.355

9.323 ± 0.465

5.129 ± 0.41

6.392 ± 0.442

1.364 ± 0.14

2.906 ± 0.105
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski