Coastal Plains virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Tibrovirus; Coastal Plains tibrovirus

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8V082|D8V082_9RHAB Matrix protein M OS=Coastal Plains virus OX=764599 GN=M PE=4 SV=1
MM1 pKa = 7.65EE2 pKa = 5.76PLSKK6 pKa = 10.74AGIKK10 pKa = 9.8IPYY13 pKa = 9.29KK14 pKa = 10.51SEE16 pKa = 3.36EE17 pKa = 4.08LMANTSDD24 pKa = 3.91VVDD27 pKa = 4.32PEE29 pKa = 4.39VDD31 pKa = 3.19NHH33 pKa = 6.25DD34 pKa = 3.62HH35 pKa = 6.96SKK37 pKa = 9.26PQSTIGDD44 pKa = 3.84IIDD47 pKa = 3.66RR48 pKa = 11.84QALNFRR54 pKa = 11.84QRR56 pKa = 11.84VSFSSSYY63 pKa = 11.59DD64 pKa = 3.44DD65 pKa = 6.01DD66 pKa = 4.35DD67 pKa = 3.93WGDD70 pKa = 4.39AILDD74 pKa = 3.75LTAKK78 pKa = 10.75DD79 pKa = 4.41KK80 pKa = 11.21DD81 pKa = 3.8DD82 pKa = 3.59KK83 pKa = 9.34TTKK86 pKa = 9.25KK87 pKa = 9.18TEE89 pKa = 3.99LTNPTKK95 pKa = 10.85LKK97 pKa = 10.36LDD99 pKa = 3.88KK100 pKa = 11.25SCTPNDD106 pKa = 3.78AACCEE111 pKa = 4.24VKK113 pKa = 10.43DD114 pKa = 5.35DD115 pKa = 5.12KK116 pKa = 11.68AKK118 pKa = 10.71EE119 pKa = 3.93DD120 pKa = 3.54WKK122 pKa = 10.54PYY124 pKa = 10.14PLLEE128 pKa = 4.45VDD130 pKa = 4.03GNDD133 pKa = 3.4PRR135 pKa = 11.84FMPKK139 pKa = 10.01LQFMLRR145 pKa = 11.84FFNIYY150 pKa = 9.44EE151 pKa = 4.36DD152 pKa = 3.54CDD154 pKa = 3.97YY155 pKa = 11.17TVEE158 pKa = 5.04DD159 pKa = 4.44YY160 pKa = 11.62NSTYY164 pKa = 10.73YY165 pKa = 10.04IYY167 pKa = 8.1PTKK170 pKa = 10.49KK171 pKa = 9.14WLNQSRR177 pKa = 11.84DD178 pKa = 3.34GTSEE182 pKa = 4.18VDD184 pKa = 4.1LQTQDD189 pKa = 4.05LYY191 pKa = 10.8IPEE194 pKa = 4.51MSDD197 pKa = 3.03HH198 pKa = 5.8YY199 pKa = 11.34QNNEE203 pKa = 3.57HH204 pKa = 7.08PLIKK208 pKa = 10.55LLITGFSVNKK218 pKa = 9.39RR219 pKa = 11.84SNIGKK224 pKa = 9.34YY225 pKa = 9.52RR226 pKa = 11.84FDD228 pKa = 3.78INNPSLNIEE237 pKa = 4.62KK238 pKa = 10.14IAEE241 pKa = 4.06IPLNEE246 pKa = 4.39IPSSINDD253 pKa = 3.54QITLIFKK260 pKa = 10.56VSGVLKK266 pKa = 10.4PMKK269 pKa = 10.43LKK271 pKa = 10.95AKK273 pKa = 8.68WW274 pKa = 3.06

Molecular weight: 31.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D8V087|D8V087_9RHAB GDP polyribonucleotidyltransferase OS=Coastal Plains virus OX=764599 GN=L PE=4 SV=1
MM1 pKa = 7.67HH2 pKa = 6.83EE3 pKa = 4.2TSSPNLRR10 pKa = 11.84FGEE13 pKa = 4.23QISNEE18 pKa = 3.88LKK20 pKa = 10.41RR21 pKa = 11.84FSEE24 pKa = 5.18SFMEE28 pKa = 4.26WWHH31 pKa = 5.84KK32 pKa = 10.83LYY34 pKa = 10.47IIVILVAGCYY44 pKa = 9.32VLHH47 pKa = 6.88KK48 pKa = 9.16VTKK51 pKa = 9.96LFSKK55 pKa = 10.25IIKK58 pKa = 9.47FISFVRR64 pKa = 11.84GKK66 pKa = 10.15IKK68 pKa = 9.21TRR70 pKa = 11.84KK71 pKa = 9.22RR72 pKa = 11.84GKK74 pKa = 8.81QGKK77 pKa = 8.66VCSHH81 pKa = 6.35KK82 pKa = 10.91FKK84 pKa = 11.29VKK86 pKa = 10.14LGKK89 pKa = 10.52RR90 pKa = 11.84NVKK93 pKa = 10.0RR94 pKa = 11.84SNLILTEE101 pKa = 4.22VTT103 pKa = 3.64

Molecular weight: 12.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

1

9

4199

33

2128

466.6

53.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.596 ± 0.326

2.024 ± 0.234

6.621 ± 0.538

5.287 ± 0.32

4.311 ± 0.405

5.12 ± 0.304

2.382 ± 0.276

8.431 ± 0.479

7.43 ± 0.717

10.264 ± 0.862

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.334 ± 0.232

5.954 ± 0.214

4.168 ± 0.437

3.525 ± 0.213

4.573 ± 0.208

7.835 ± 0.257

5.597 ± 0.263

4.93 ± 0.608

1.643 ± 0.099

3.977 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski