Tenacibaculum sp. M341

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Tenacibaculum; unclassified Tenacibaculum

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R1A672|A0A4R1A672_9FLAO Uncharacterized protein OS=Tenacibaculum sp. M341 OX=2530339 GN=EYW44_15235 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 9.06KK3 pKa = 8.21TLLFLIYY10 pKa = 10.35FSLILFANAQDD21 pKa = 4.05GASTCDD27 pKa = 3.29AAEE30 pKa = 4.27PMCSDD35 pKa = 3.86DD36 pKa = 3.76MGVKK40 pKa = 10.0IFDD43 pKa = 3.86NVTGKK48 pKa = 9.98PNTGTIACLSTTPNPAWFFIRR69 pKa = 11.84VEE71 pKa = 3.93SSGRR75 pKa = 11.84LEE77 pKa = 3.89FQIIQAQDD85 pKa = 2.55FDD87 pKa = 3.96AAGNVVGNTLDD98 pKa = 3.42VDD100 pKa = 4.64FTSWGPFTAPDD111 pKa = 4.29GNCSSLATTCVDD123 pKa = 3.23TTGAPTTCVDD133 pKa = 3.15NTFDD137 pKa = 3.84PDD139 pKa = 3.84YY140 pKa = 10.86YY141 pKa = 10.49INNEE145 pKa = 4.18DD146 pKa = 3.53GTNIMDD152 pKa = 4.24CSYY155 pKa = 10.68SGSAIEE161 pKa = 5.58SFTIEE166 pKa = 3.87NAVAGEE172 pKa = 4.04FYY174 pKa = 10.63ILLITNFQDD183 pKa = 3.58SPGKK187 pKa = 10.24IKK189 pKa = 10.94LEE191 pKa = 3.88QTNLGTTGAGEE202 pKa = 4.31TDD204 pKa = 3.58CSIIAGEE211 pKa = 4.66LGPDD215 pKa = 3.16QTVCDD220 pKa = 4.26GTTITLDD227 pKa = 3.52GTPSTGTATSYY238 pKa = 10.07EE239 pKa = 4.07WQIDD243 pKa = 3.45TGAGFTTIAGEE254 pKa = 4.24TGNTLTINNDD264 pKa = 2.51KK265 pKa = 10.95SGTYY269 pKa = 9.62RR270 pKa = 11.84VIITDD275 pKa = 3.8DD276 pKa = 3.5VGGTATDD283 pKa = 5.05DD284 pKa = 3.19IDD286 pKa = 3.68ITFLPVPVANKK297 pKa = 9.28PADD300 pKa = 3.5INFCDD305 pKa = 3.8SDD307 pKa = 4.0SDD309 pKa = 3.99GFHH312 pKa = 6.61TFDD315 pKa = 4.36LQNDD319 pKa = 3.69VTPLVLGAQSAIQFEE334 pKa = 4.75VVYY337 pKa = 10.15FLSQTDD343 pKa = 3.29ADD345 pKa = 4.33NNNTANALANPYY357 pKa = 9.36TNSTANSTQTIYY369 pKa = 11.41ARR371 pKa = 11.84IHH373 pKa = 5.22NTSNPDD379 pKa = 2.96TCFDD383 pKa = 3.66TTSFSLDD390 pKa = 3.2INSLPTPRR398 pKa = 11.84QPSDD402 pKa = 2.99IVVCNDD408 pKa = 3.29LGNPVGFYY416 pKa = 11.23NNFILATKK424 pKa = 10.29DD425 pKa = 3.39NEE427 pKa = 4.24ILGGLSPTQYY437 pKa = 10.25SVSYY441 pKa = 8.43HH442 pKa = 4.65TTLAGAQTNATTDD455 pKa = 3.68VIDD458 pKa = 4.63KK459 pKa = 10.73NIAYY463 pKa = 8.86TNIIPNEE470 pKa = 3.64QVIFVRR476 pKa = 11.84VEE478 pKa = 3.71NSSNTSCSVATTVSSATFKK497 pKa = 10.64PFKK500 pKa = 10.52LVVNPLPVIINPIVQIEE517 pKa = 4.07QCEE520 pKa = 3.99NDD522 pKa = 3.42GDD524 pKa = 3.92FTAIINLTQAQINISNNHH542 pKa = 5.61LNEE545 pKa = 3.78TFKK548 pKa = 11.29YY549 pKa = 7.77YY550 pKa = 10.36TSEE553 pKa = 3.73VDD555 pKa = 3.81ANTDD559 pKa = 3.34NAPIADD565 pKa = 5.05PINYY569 pKa = 8.23NATNGEE575 pKa = 4.58VVWVRR580 pKa = 11.84TISDD584 pKa = 3.48KK585 pKa = 11.5DD586 pKa = 3.68CFRR589 pKa = 11.84VSQINVIISFAADD602 pKa = 2.69IMYY605 pKa = 10.47DD606 pKa = 3.92KK607 pKa = 11.07EE608 pKa = 4.14FTEE611 pKa = 5.7CDD613 pKa = 3.61DD614 pKa = 5.46FLDD617 pKa = 4.51ADD619 pKa = 4.31GNNTPSNDD627 pKa = 3.54DD628 pKa = 3.19TDD630 pKa = 5.56GITNFDD636 pKa = 3.42FSIAEE641 pKa = 4.05GEE643 pKa = 4.27IINLFDD649 pKa = 3.61PAIRR653 pKa = 11.84SDD655 pKa = 3.63LKK657 pKa = 11.07VLFFEE662 pKa = 4.65TQADD666 pKa = 3.76RR667 pKa = 11.84DD668 pKa = 3.84AVTNEE673 pKa = 3.36IADD676 pKa = 3.29ISKK679 pKa = 10.24YY680 pKa = 10.66RR681 pKa = 11.84NTNFPGLASNTIYY694 pKa = 10.55IKK696 pKa = 10.43IINKK700 pKa = 9.41INNNCTGLSKK710 pKa = 10.92LFVRR714 pKa = 11.84VLPLPSFDD722 pKa = 3.43VTSPQILCLNNLSSIEE738 pKa = 4.04AEE740 pKa = 4.5SPGDD744 pKa = 3.37TYY746 pKa = 11.42AYY748 pKa = 9.07EE749 pKa = 3.88WTRR752 pKa = 11.84NGNATVIGNNQTLNLTQGGEE772 pKa = 4.34YY773 pKa = 10.26KK774 pKa = 9.46VTAINTTTLCKK785 pKa = 9.62RR786 pKa = 11.84SRR788 pKa = 11.84IIFVNEE794 pKa = 3.93SIIATINQNDD804 pKa = 3.51VSIIDD809 pKa = 4.64DD810 pKa = 3.88SDD812 pKa = 4.04NNTITINNDD821 pKa = 2.84TGNLGIGAYY830 pKa = 9.42EE831 pKa = 4.52FALQDD836 pKa = 3.33EE837 pKa = 4.92NGQIVQDD844 pKa = 4.09YY845 pKa = 9.8QDD847 pKa = 3.45EE848 pKa = 4.58PKK850 pKa = 10.66FEE852 pKa = 4.07NLQGGIYY859 pKa = 9.58TILVRR864 pKa = 11.84DD865 pKa = 4.09KK866 pKa = 10.02NNCGIAEE873 pKa = 4.48LDD875 pKa = 3.65VSVLEE880 pKa = 4.7FPDD883 pKa = 4.53FFTPNEE889 pKa = 3.96DD890 pKa = 4.96SINDD894 pKa = 3.36TWNVKK899 pKa = 8.94GANSLFYY906 pKa = 9.56PSNSIYY912 pKa = 10.61IFDD915 pKa = 4.41RR916 pKa = 11.84YY917 pKa = 10.64GKK919 pKa = 10.32LLTSLKK925 pKa = 10.46VDD927 pKa = 3.49DD928 pKa = 4.45QGWNGTYY935 pKa = 10.36NGNALPSNDD944 pKa = 2.8YY945 pKa = 9.62WFNIEE950 pKa = 3.88LTDD953 pKa = 4.13RR954 pKa = 11.84NGNVITRR961 pKa = 11.84KK962 pKa = 9.6GHH964 pKa = 6.3FSLLLDD970 pKa = 3.79

Molecular weight: 105.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R1A964|A0A4R1A964_9FLAO DUF1508 domain-containing protein OS=Tenacibaculum sp. M341 OX=2530339 GN=EYW44_09960 PE=4 SV=1
EEE2 pKa = 4.63FNAKKK7 pKa = 9.26KKK9 pKa = 10.71FRR11 pKa = 11.84AQFRR15 pKa = 11.84GVRR18 pKa = 11.84NVEEE22 pKa = 3.75FLYYY26 pKa = 10.6LTQIFA

Molecular weight: 3.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4253

0

4253

1484396

22

8406

349.0

39.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.595 ± 0.036

0.754 ± 0.014

5.538 ± 0.031

6.727 ± 0.042

5.372 ± 0.031

6.067 ± 0.039

1.66 ± 0.018

8.171 ± 0.041

8.504 ± 0.064

9.025 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.863 ± 0.018

6.989 ± 0.05

3.096 ± 0.023

3.192 ± 0.022

3.198 ± 0.024

6.823 ± 0.038

6.049 ± 0.051

6.179 ± 0.032

1.035 ± 0.012

4.164 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski