Bacillus phage vB_BpsS-36

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3BWX1|A0A3G3BWX1_9CAUD Uncharacterized protein OS=Bacillus phage vB_BpsS-36 OX=2419622 GN=BpsS36_00002 PE=4 SV=1
MM1 pKa = 7.01IAVYY5 pKa = 10.38VLSNNSSVNVYY16 pKa = 9.87RR17 pKa = 11.84VEE19 pKa = 4.08HH20 pKa = 6.54GIDD23 pKa = 3.63DD24 pKa = 3.93EE25 pKa = 4.64VLAGLSGTAPEE36 pKa = 4.23WCKK39 pKa = 10.83VEE41 pKa = 4.24YY42 pKa = 10.73DD43 pKa = 3.62DD44 pKa = 6.45DD45 pKa = 4.11RR46 pKa = 11.84AFFKK50 pKa = 11.02VGEE53 pKa = 4.18LTVYY57 pKa = 10.61LDD59 pKa = 2.89EE60 pKa = 4.82CMRR63 pKa = 11.84VV64 pKa = 3.03

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3BX24|A0A3G3BX24_9CAUD Uncharacterized protein OS=Bacillus phage vB_BpsS-36 OX=2419622 GN=BpsS36_00062 PE=4 SV=1
MM1 pKa = 7.37YY2 pKa = 10.8SFGTKK7 pKa = 7.66ITKK10 pKa = 10.06KK11 pKa = 10.09GDD13 pKa = 3.16MVTVQRR19 pKa = 11.84GLYY22 pKa = 9.68KK23 pKa = 10.63RR24 pKa = 11.84LTAHH28 pKa = 7.65KK29 pKa = 9.52DD30 pKa = 3.31QLKK33 pKa = 9.97SATVEE38 pKa = 3.86RR39 pKa = 11.84RR40 pKa = 11.84VLGGWCLVIYY50 pKa = 10.43YY51 pKa = 9.78GGKK54 pKa = 8.51AHH56 pKa = 7.45RR57 pKa = 11.84FKK59 pKa = 10.93HH60 pKa = 5.04VSKK63 pKa = 11.03GGAEE67 pKa = 3.86RR68 pKa = 11.84SLQKK72 pKa = 10.83LLQLTKK78 pKa = 10.89

Molecular weight: 8.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

15138

38

1028

225.9

25.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.368 ± 0.316

0.879 ± 0.129

5.998 ± 0.192

8.409 ± 0.291

4.294 ± 0.194

7.815 ± 0.656

2.107 ± 0.153

5.509 ± 0.264

8.145 ± 0.502

7.894 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.907 ± 0.231

4.538 ± 0.291

3.428 ± 0.321

3.627 ± 0.216

4.935 ± 0.26

5.681 ± 0.274

5.826 ± 0.353

6.698 ± 0.266

1.242 ± 0.118

3.699 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski