Clostridium sp. CAG:1024

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1915 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5AI68|R5AI68_9CLOT Phospho-N-acetylmuramoyl-pentapeptide-transferase OS=Clostridium sp. CAG:1024 OX=1262770 GN=mraY PE=3 SV=1
MM1 pKa = 7.78KK2 pKa = 9.33LTKK5 pKa = 9.61TVSLLAAVLLLVLSLTACQFGTLPSAGTAATPAEE39 pKa = 4.33DD40 pKa = 3.97TQDD43 pKa = 3.17PTMVYY48 pKa = 10.43LPDD51 pKa = 3.36TAAPVQGEE59 pKa = 4.51STDD62 pKa = 3.82TPDD65 pKa = 5.92DD66 pKa = 4.04DD67 pKa = 4.41AQRR70 pKa = 11.84LAAYY74 pKa = 10.02RR75 pKa = 11.84EE76 pKa = 4.35ALSSFYY82 pKa = 10.3STGRR86 pKa = 11.84FPDD89 pKa = 4.03GTDD92 pKa = 2.75SGYY95 pKa = 10.91SADD98 pKa = 3.83FGEE101 pKa = 4.35ITGNRR106 pKa = 11.84FAICDD111 pKa = 3.57VDD113 pKa = 4.66GDD115 pKa = 4.35GADD118 pKa = 3.61EE119 pKa = 5.72LILQFVTAPVAGQLEE134 pKa = 4.18IVYY137 pKa = 10.04RR138 pKa = 11.84YY139 pKa = 7.84DD140 pKa = 3.42TASGALVSEE149 pKa = 4.48LSEE152 pKa = 4.24YY153 pKa = 10.37PPLSYY158 pKa = 10.55GANGSVTALFSHH170 pKa = 5.98NQGLSGGDD178 pKa = 2.94FWPYY182 pKa = 9.85HH183 pKa = 6.47RR184 pKa = 11.84YY185 pKa = 9.95VFDD188 pKa = 4.65AAAQSYY194 pKa = 7.56TLEE197 pKa = 4.26ATVDD201 pKa = 2.89AWSRR205 pKa = 11.84AVSDD209 pKa = 3.68TDD211 pKa = 4.08FDD213 pKa = 4.84GNPYY217 pKa = 10.31PNDD220 pKa = 3.22IDD222 pKa = 4.73RR223 pKa = 11.84EE224 pKa = 4.37GAGIVYY230 pKa = 10.18LVTEE234 pKa = 4.34NGSTNTLSKK243 pKa = 10.64SDD245 pKa = 3.83YY246 pKa = 10.09EE247 pKa = 4.34SWLMSHH253 pKa = 7.47PSGAAILVPYY263 pKa = 10.08TEE265 pKa = 4.51LTEE268 pKa = 4.15SAIAAIGEE276 pKa = 4.34TAVNEE281 pKa = 3.94ISLSLFADD289 pKa = 3.68SAEE292 pKa = 4.11GLAALLDD299 pKa = 4.43NINARR304 pKa = 11.84VEE306 pKa = 4.1VGTAGSSLKK315 pKa = 9.57ATYY318 pKa = 10.21AAGQLLDD325 pKa = 3.19WCAATKK331 pKa = 10.64LSADD335 pKa = 3.98DD336 pKa = 3.63VRR338 pKa = 11.84AATLAWMVNLGNDD351 pKa = 3.37AQIAFAEE358 pKa = 4.28KK359 pKa = 10.22LALVNAACSSLRR371 pKa = 11.84TDD373 pKa = 3.56KK374 pKa = 11.38GADD377 pKa = 3.28LMSEE381 pKa = 4.06AGYY384 pKa = 9.8PSAAYY389 pKa = 8.47PWTTLSPEE397 pKa = 4.19TLSSIMEE404 pKa = 4.19AVGVEE409 pKa = 4.0

Molecular weight: 42.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5AE72|R5AE72_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:1024 OX=1262770 GN=BN454_00847 PE=4 SV=1
MM1 pKa = 6.83TRR3 pKa = 11.84AAARR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84AAIRR14 pKa = 11.84RR15 pKa = 11.84TITTSRR21 pKa = 11.84CPATDD26 pKa = 3.34RR27 pKa = 11.84VSCSCRR33 pKa = 11.84RR34 pKa = 11.84CKK36 pKa = 9.95WPALSRR42 pKa = 11.84PMRR45 pKa = 11.84TTAISWSPMWSIPSGMRR62 pKa = 11.84TARR65 pKa = 11.84TTTSCPRR72 pKa = 11.84PNRR75 pKa = 11.84ASGKK79 pKa = 8.49RR80 pKa = 11.84ARR82 pKa = 11.84SSRR85 pKa = 11.84ARR87 pKa = 11.84SIRR90 pKa = 11.84STRR93 pKa = 11.84PSQRR97 pKa = 11.84SATSARR103 pKa = 11.84RR104 pKa = 11.84SLCRR108 pKa = 11.84GSSTARR114 pKa = 11.84AISTPSATSSSARR127 pKa = 11.84RR128 pKa = 11.84VRR130 pKa = 11.84RR131 pKa = 11.84RR132 pKa = 11.84SRR134 pKa = 11.84TTNPRR139 pKa = 11.84SSAGSSPGATAWSRR153 pKa = 11.84RR154 pKa = 11.84RR155 pKa = 11.84RR156 pKa = 11.84GSAASCWRR164 pKa = 11.84SII166 pKa = 4.18

Molecular weight: 18.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1915

0

1915

549254

29

1928

286.8

31.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.335 ± 0.071

1.728 ± 0.024

5.503 ± 0.048

6.662 ± 0.058

3.836 ± 0.035

7.674 ± 0.049

1.883 ± 0.03

5.817 ± 0.051

4.602 ± 0.053

10.115 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.767 ± 0.029

3.223 ± 0.036

4.056 ± 0.032

2.98 ± 0.028

6.311 ± 0.072

5.494 ± 0.049

5.548 ± 0.052

7.178 ± 0.047

0.846 ± 0.021

3.433 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski