Mink coronavirus strain WD1133

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Minacovirus; unclassified Minacovirus

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9J205|D9J205_9ALPC Envelope small membrane protein OS=Mink coronavirus strain WD1133 OX=766792 GN=E PE=3 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84KK3 pKa = 8.29MLLILVLIPCVLGDD17 pKa = 4.58LFPCRR22 pKa = 11.84TSINSTIGNHH32 pKa = 5.97WYY34 pKa = 10.72LIDD37 pKa = 4.27NFVKK41 pKa = 10.46NYY43 pKa = 10.33SVSLPSNSDD52 pKa = 3.34VVLGDD57 pKa = 3.26YY58 pKa = 10.61FPTVEE63 pKa = 4.18PWHH66 pKa = 6.28NCLLEE71 pKa = 4.16QTNSTVIFDD80 pKa = 4.37GLRR83 pKa = 11.84ALYY86 pKa = 9.33WDD88 pKa = 4.1YY89 pKa = 11.93NNGQVPVRR97 pKa = 11.84RR98 pKa = 11.84YY99 pKa = 10.02LAVAVYY105 pKa = 10.0GDD107 pKa = 4.25PYY109 pKa = 10.74SVSVYY114 pKa = 8.42FTLDD118 pKa = 3.25YY119 pKa = 10.8EE120 pKa = 5.05GPRR123 pKa = 11.84GAKK126 pKa = 9.11ICLCKK131 pKa = 10.58GMPQPTHH138 pKa = 6.4ISGEE142 pKa = 4.21ANCWSSQCKK151 pKa = 9.37LNHH154 pKa = 6.13YY155 pKa = 10.06FPICNNTDD163 pKa = 2.95HH164 pKa = 7.17CGHH167 pKa = 6.94FLYY170 pKa = 10.64GLKK173 pKa = 9.89WSQTEE178 pKa = 4.02LFAYY182 pKa = 10.43LYY184 pKa = 10.73GYY186 pKa = 9.96VYY188 pKa = 10.61NIKK191 pKa = 10.36LVNHH195 pKa = 6.93WFNNASLALMRR206 pKa = 11.84AASVGQEE213 pKa = 3.99GWWFNPVRR221 pKa = 11.84NLTYY225 pKa = 10.24YY226 pKa = 10.4SVYY229 pKa = 10.3KK230 pKa = 10.43NSSSTIVQNCTGNCEE245 pKa = 3.95EE246 pKa = 4.31YY247 pKa = 11.19ANNIFSTEE255 pKa = 3.76PGGIIPEE262 pKa = 4.11GFSFNNWFVLTNDD275 pKa = 3.19STVLDD280 pKa = 3.84GRR282 pKa = 11.84FLTRR286 pKa = 11.84QPLLVNCLWPVPSFSEE302 pKa = 4.18ISQEE306 pKa = 4.03FCFASADD313 pKa = 3.6FTQCNGYY320 pKa = 9.69KK321 pKa = 10.34LNSTADD327 pKa = 3.52VIRR330 pKa = 11.84FNLNFTHH337 pKa = 5.99EE338 pKa = 4.17TAAASGTTFFEE349 pKa = 5.07LNTTGGVVLYY359 pKa = 9.41ISCYY363 pKa = 7.7NQSRR367 pKa = 11.84TEE369 pKa = 4.0AQVSEE374 pKa = 4.37GFVPFGNHH382 pKa = 5.91EE383 pKa = 4.26GSLYY387 pKa = 10.72CYY389 pKa = 10.34VSYY392 pKa = 11.57NEE394 pKa = 4.17TLQKK398 pKa = 10.44FLGVLPPSVKK408 pKa = 10.27EE409 pKa = 3.62IAISKK414 pKa = 10.69DD415 pKa = 2.58GGFYY419 pKa = 10.48INGYY423 pKa = 10.47NYY425 pKa = 10.1FQTFPIDD432 pKa = 4.1CISFNLTTGNTGAFWTIAYY451 pKa = 7.72TSYY454 pKa = 10.56TDD456 pKa = 3.61VMVDD460 pKa = 3.61VEE462 pKa = 4.15NTAIKK467 pKa = 10.2RR468 pKa = 11.84VIYY471 pKa = 10.15CNSHH475 pKa = 6.8INDD478 pKa = 3.1IRR480 pKa = 11.84CNQLTPSLPDD490 pKa = 2.79GFYY493 pKa = 10.32PVSPKK498 pKa = 10.76AIGFINKK505 pKa = 8.24TFVTLPANFDD515 pKa = 3.43HH516 pKa = 7.08VYY518 pKa = 11.08VNITGNVNLKK528 pKa = 10.46AEE530 pKa = 4.3RR531 pKa = 11.84GRR533 pKa = 11.84PYY535 pKa = 11.28NNGGNATLSFQGCIAVSQFTVNLNHH560 pKa = 6.89TCTITNAGSSGCTNAYY576 pKa = 7.31EE577 pKa = 4.29ANVYY581 pKa = 7.1MTSGSCPFAFDD592 pKa = 3.76RR593 pKa = 11.84LNNHH597 pKa = 5.58MSFSKK602 pKa = 10.76LCFSTVPLGDD612 pKa = 3.83DD613 pKa = 3.64CRR615 pKa = 11.84LDD617 pKa = 3.37LTVQTRR623 pKa = 11.84YY624 pKa = 7.08FTGVFAHH631 pKa = 6.54VYY633 pKa = 9.21VSYY636 pKa = 11.19KK637 pKa = 10.32FGLDD641 pKa = 2.87IVGLPTSDD649 pKa = 5.14AGLKK653 pKa = 10.16DD654 pKa = 4.12LSVLHH659 pKa = 6.59LNVCTEE665 pKa = 3.88YY666 pKa = 11.03NVYY669 pKa = 10.29GFSGTGIIRR678 pKa = 11.84QTNQSIPGGLYY689 pKa = 8.24YY690 pKa = 10.59TSLSGDD696 pKa = 3.35LLGFKK701 pKa = 10.35NVTTGTVYY709 pKa = 10.73SITPCEE715 pKa = 4.22VSAQAAVIDD724 pKa = 4.3GSIVGAITSVNSEE737 pKa = 4.03LLGLKK742 pKa = 9.63NHH744 pKa = 6.14ITTPYY749 pKa = 7.97FYY751 pKa = 10.62YY752 pKa = 10.66YY753 pKa = 10.65SVYY756 pKa = 10.8NYY758 pKa = 9.01TADD761 pKa = 3.3NVTRR765 pKa = 11.84SMQKK769 pKa = 10.27YY770 pKa = 9.57DD771 pKa = 3.67VNCTPVISYY780 pKa = 11.43SNMGVCANGALVFINVTHH798 pKa = 6.73TNGDD802 pKa = 3.57VQPISTGNVTIPSNFTISVQMEE824 pKa = 4.04YY825 pKa = 10.78VQVSTEE831 pKa = 4.19LVSIDD836 pKa = 3.32CARR839 pKa = 11.84YY840 pKa = 8.63VCNGNARR847 pKa = 11.84CGKK850 pKa = 9.88LLSQYY855 pKa = 10.68VSACHH860 pKa = 6.31TIEE863 pKa = 3.73QALAMGSRR871 pKa = 11.84LEE873 pKa = 4.13SMEE876 pKa = 4.5LEE878 pKa = 4.22SMISISANALKK889 pKa = 10.29LASVEE894 pKa = 4.2EE895 pKa = 4.54FNSSAMLNPVYY906 pKa = 10.55NEE908 pKa = 3.91SGNTIGGIYY917 pKa = 10.18LDD919 pKa = 4.0GLKK922 pKa = 10.68DD923 pKa = 3.19ILPRR927 pKa = 11.84KK928 pKa = 8.52NKK930 pKa = 9.76HH931 pKa = 4.91GSSRR935 pKa = 11.84STIEE939 pKa = 4.47DD940 pKa = 3.63LLFNKK945 pKa = 9.4VVTTGLGTVDD955 pKa = 4.12EE956 pKa = 4.86DD957 pKa = 4.55YY958 pKa = 11.25KK959 pKa = 11.14RR960 pKa = 11.84CTKK963 pKa = 10.68GSDD966 pKa = 3.08IADD969 pKa = 4.35LACAQYY975 pKa = 11.34YY976 pKa = 9.2NGIMVLPGVANDD988 pKa = 4.86GKK990 pKa = 10.3MSMYY994 pKa = 9.3TASLSGGISLGALGGGAVAIPFSLAVQARR1023 pKa = 11.84LNYY1026 pKa = 9.72VALQTDD1032 pKa = 3.9VLQRR1036 pKa = 11.84NQEE1039 pKa = 3.65ILAASFNQAIGNITIALGKK1058 pKa = 9.82VNNAIYY1064 pKa = 8.4QTSQSLSTVAQALTKK1079 pKa = 10.33VQDD1082 pKa = 3.72VVNSQGKK1089 pKa = 9.06ALNHH1093 pKa = 6.2LTLQLQNNFQAISSSIQDD1111 pKa = 3.17IYY1113 pKa = 11.73YY1114 pKa = 10.85KK1115 pKa = 10.67LDD1117 pKa = 5.03DD1118 pKa = 4.46INADD1122 pKa = 3.5AQVDD1126 pKa = 3.81RR1127 pKa = 11.84LITGRR1132 pKa = 11.84LAALNAFVTQTLTRR1146 pKa = 11.84QAEE1149 pKa = 4.39VRR1151 pKa = 11.84ASRR1154 pKa = 11.84QLAKK1158 pKa = 10.58EE1159 pKa = 3.99KK1160 pKa = 10.98VNEE1163 pKa = 4.29CVRR1166 pKa = 11.84SQSSRR1171 pKa = 11.84FGFCGNGTHH1180 pKa = 7.6LFSLTNAAPNGMVFFHH1196 pKa = 6.23TVLVPTAYY1204 pKa = 8.26EE1205 pKa = 4.24TVTAWSGICASDD1217 pKa = 3.87DD1218 pKa = 3.36SRR1220 pKa = 11.84TFGLIVKK1227 pKa = 9.68DD1228 pKa = 3.7VSLTLFRR1235 pKa = 11.84NHH1237 pKa = 7.22DD1238 pKa = 3.7GKK1240 pKa = 11.2FYY1242 pKa = 9.39LTPRR1246 pKa = 11.84TMYY1249 pKa = 9.62QPRR1252 pKa = 11.84VATSADD1258 pKa = 3.12FVQIVDD1264 pKa = 3.89CDD1266 pKa = 3.88VLFVNATILEE1276 pKa = 4.42LPSIVPDD1283 pKa = 3.83YY1284 pKa = 11.26IDD1286 pKa = 3.69INKK1289 pKa = 8.34TVQDD1293 pKa = 4.57LLDD1296 pKa = 3.85SYY1298 pKa = 10.94KK1299 pKa = 10.87PNWTVPEE1306 pKa = 4.33FSLDD1310 pKa = 3.4IFNQTYY1316 pKa = 11.1LNITNEE1322 pKa = 3.9INDD1325 pKa = 3.86LEE1327 pKa = 4.24NRR1329 pKa = 11.84SVVLYY1334 pKa = 8.17NTTKK1338 pKa = 10.47EE1339 pKa = 4.08LEE1341 pKa = 4.19LLIQSINNTLVDD1353 pKa = 4.7LEE1355 pKa = 4.39WLNKK1359 pKa = 9.78IEE1361 pKa = 5.23TYY1363 pKa = 10.77VKK1365 pKa = 8.7WPWYY1369 pKa = 8.59VWVLIALIFLFVLPMLLFCCLSTGCCGCCGCLSSCAAGCCKK1410 pKa = 10.4YY1411 pKa = 10.81GCSRR1415 pKa = 11.84DD1416 pKa = 3.43LSRR1419 pKa = 11.84YY1420 pKa = 8.12EE1421 pKa = 4.39PIEE1424 pKa = 4.11KK1425 pKa = 10.11VHH1427 pKa = 6.2VNN1429 pKa = 3.28

Molecular weight: 157.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9J210|D9J210_9ALPC Accessory protein 7b OS=Mink coronavirus strain WD1133 OX=766792 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84FNLITLIPGSVWFKK16 pKa = 10.69LPRR19 pKa = 11.84PFKK22 pKa = 8.89NWIVAKK28 pKa = 9.87VKK30 pKa = 10.58RR31 pKa = 11.84KK32 pKa = 10.15VPTCRR37 pKa = 11.84SIKK40 pKa = 8.29TDD42 pKa = 3.06YY43 pKa = 10.32RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84AIYY49 pKa = 10.08NLRR52 pKa = 11.84IKK54 pKa = 10.42NASTNSCNSFSSSSCFRR71 pKa = 11.84NKK73 pKa = 10.29

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

13206

73

6670

1467.3

163.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.247 ± 0.14

3.589 ± 0.276

5.498 ± 0.514

3.953 ± 0.192

5.225 ± 0.185

6.558 ± 0.182

1.833 ± 0.118

5.369 ± 0.295

6.05 ± 0.669

8.595 ± 0.547

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.199

6.179 ± 0.596

3.355 ± 0.206

2.787 ± 0.291

3.249 ± 0.412

7.065 ± 0.414

6.171 ± 0.334

9.935 ± 0.612

1.227 ± 0.22

5.013 ± 0.254
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski