Bovine faeces associated smacovirus 6

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Bostasmacovirus; Bostasmacovirus bovas1

Average proteome isoelectric point is 5.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A168MFS2|A0A168MFS2_9VIRU Putative capsid protein OS=Bovine faeces associated smacovirus 6 OX=1843754 PE=4 SV=1
MM1 pKa = 6.94ATQYY5 pKa = 11.34AKK7 pKa = 10.44SSYY10 pKa = 10.34SEE12 pKa = 4.34VYY14 pKa = 10.84DD15 pKa = 3.24MHH17 pKa = 7.14TEE19 pKa = 4.32FGKK22 pKa = 7.6TTLIGIHH29 pKa = 6.07TPTTNRR35 pKa = 11.84WQKK38 pKa = 9.8YY39 pKa = 6.22MRR41 pKa = 11.84GFCHH45 pKa = 5.38QFKK48 pKa = 10.58KK49 pKa = 10.64FRR51 pKa = 11.84YY52 pKa = 8.44QGASLSFVPVATLPVDD68 pKa = 3.81PLAVSYY74 pKa = 10.74EE75 pKa = 4.07GGEE78 pKa = 4.04AQIDD82 pKa = 3.82PRR84 pKa = 11.84DD85 pKa = 3.73MVNPIIHH92 pKa = 6.59CGMRR96 pKa = 11.84GEE98 pKa = 4.77SMGKK102 pKa = 8.68YY103 pKa = 9.53LDD105 pKa = 4.55EE106 pKa = 4.35IFHH109 pKa = 6.92FNLEE113 pKa = 4.25HH114 pKa = 7.0KK115 pKa = 10.56LNSVDD120 pKa = 3.31VANVVNEE127 pKa = 4.13DD128 pKa = 4.92LIADD132 pKa = 4.84LEE134 pKa = 4.26NMYY137 pKa = 8.89YY138 pKa = 9.66TALSSPAFKK147 pKa = 10.32KK148 pKa = 10.05SHH150 pKa = 5.8PQQGFRR156 pKa = 11.84KK157 pKa = 9.38SGLHH161 pKa = 5.44PMVYY165 pKa = 10.37SLATDD170 pKa = 3.68RR171 pKa = 11.84PKK173 pKa = 11.12NFSLPDD179 pKa = 3.42TDD181 pKa = 3.75TDD183 pKa = 3.91ADD185 pKa = 4.1ALPSSTAGPLSGEE198 pKa = 4.14PGGNATDD205 pKa = 3.73GFYY208 pKa = 11.18GSFGVPYY215 pKa = 9.87AQARR219 pKa = 11.84MQVDD223 pKa = 3.35YY224 pKa = 11.0GFNGVEE230 pKa = 4.29TQRR233 pKa = 11.84QFPQLFTNHH242 pKa = 5.92AQSLGWLDD250 pKa = 3.22TMQNYY255 pKa = 10.43SIYY258 pKa = 10.26SEE260 pKa = 4.38LGGVPAGTTEE270 pKa = 3.88QYY272 pKa = 10.0LTGLPKK278 pKa = 9.31IYY280 pKa = 9.16MYY282 pKa = 10.95FVMLPPAYY290 pKa = 8.7KK291 pKa = 8.93TEE293 pKa = 4.05LYY295 pKa = 9.83FRR297 pKa = 11.84VIIRR301 pKa = 11.84HH302 pKa = 4.66FFEE305 pKa = 4.4FKK307 pKa = 10.05EE308 pKa = 4.13YY309 pKa = 11.0RR310 pKa = 11.84NMIQPFAMEE319 pKa = 4.97TINGSAYY326 pKa = 10.68DD327 pKa = 4.12GVLEE331 pKa = 4.2TAPVDD336 pKa = 3.34QAMALRR342 pKa = 11.84MEE344 pKa = 4.49NATLDD349 pKa = 3.87VVNGEE354 pKa = 4.2ASLMSDD360 pKa = 3.96GSSS363 pKa = 2.87

Molecular weight: 40.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A168MFS2|A0A168MFS2_9VIRU Putative capsid protein OS=Bovine faeces associated smacovirus 6 OX=1843754 PE=4 SV=1
MM1 pKa = 7.85SDD3 pKa = 3.61PKK5 pKa = 10.28WYY7 pKa = 10.21DD8 pKa = 2.95VTAPQAVLSAEE19 pKa = 4.03NVEE22 pKa = 4.68TILEE26 pKa = 4.01QLEE29 pKa = 4.37CEE31 pKa = 4.17RR32 pKa = 11.84YY33 pKa = 9.8AYY35 pKa = 10.34ADD37 pKa = 3.47EE38 pKa = 4.42VGEE41 pKa = 4.15GGYY44 pKa = 9.45KK45 pKa = 9.59HH46 pKa = 5.14WQIRR50 pKa = 11.84YY51 pKa = 7.43VLRR54 pKa = 11.84KK55 pKa = 9.02GSPVEE60 pKa = 3.85EE61 pKa = 4.55QIMIWSMWKK70 pKa = 9.11AHH72 pKa = 5.71VSPTHH77 pKa = 5.33VRR79 pKa = 11.84NFNYY83 pKa = 9.73IMKK86 pKa = 8.88TDD88 pKa = 3.8NYY90 pKa = 8.06FCSWEE95 pKa = 4.17SEE97 pKa = 3.99LRR99 pKa = 11.84EE100 pKa = 4.01YY101 pKa = 10.69RR102 pKa = 11.84YY103 pKa = 10.5LEE105 pKa = 3.65YY106 pKa = 10.67RR107 pKa = 11.84PWQKK111 pKa = 9.45YY112 pKa = 4.1TLEE115 pKa = 4.54MIEE118 pKa = 3.95QQNDD122 pKa = 3.07RR123 pKa = 11.84EE124 pKa = 4.28IFVIQDD130 pKa = 3.49PLGGIGKK137 pKa = 6.53TTFAKK142 pKa = 10.54HH143 pKa = 5.67LVANHH148 pKa = 5.78KK149 pKa = 10.35AIYY152 pKa = 9.71IPPLGEE158 pKa = 3.93GQEE161 pKa = 4.12YY162 pKa = 8.47MAMAMAKK169 pKa = 9.65ARR171 pKa = 11.84EE172 pKa = 4.2GRR174 pKa = 11.84RR175 pKa = 11.84EE176 pKa = 3.81TFIIDD181 pKa = 3.37VPRR184 pKa = 11.84AEE186 pKa = 4.26SLKK189 pKa = 10.48KK190 pKa = 9.95KK191 pKa = 10.32AGMWSAVEE199 pKa = 3.91QIKK202 pKa = 11.15NGFLYY207 pKa = 10.45DD208 pKa = 4.91KK209 pKa = 10.29RR210 pKa = 11.84YY211 pKa = 8.69QWSEE215 pKa = 3.4KK216 pKa = 8.98WIKK219 pKa = 10.02SPKK222 pKa = 9.72IVVLTNEE229 pKa = 4.11TEE231 pKa = 4.08LPKK234 pKa = 10.98DD235 pKa = 3.32KK236 pKa = 10.91LSNDD240 pKa = 2.94RR241 pKa = 11.84WNVYY245 pKa = 9.4QPGNFEE251 pKa = 4.16YY252 pKa = 10.94

Molecular weight: 29.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

615

252

363

307.5

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.992 ± 0.412

0.65 ± 0.092

4.878 ± 0.583

7.48 ± 1.819

4.553 ± 1.138

6.829 ± 1.071

2.439 ± 0.292

4.715 ± 1.047

5.528 ± 1.543

6.829 ± 0.562

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.228 ± 0.421

4.39 ± 0.016

5.528 ± 0.491

4.715 ± 0.03

4.39 ± 0.747

6.179 ± 0.908

5.528 ± 1.0

5.854 ± 0.063

1.951 ± 1.293

6.341 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski