Vibrio phage H188

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1LJB5|A0A0K1LJB5_9CAUD Uncharacterized protein OS=Vibrio phage H188 OX=1673892 PE=4 SV=1
MM1 pKa = 7.04KK2 pKa = 9.7TVEE5 pKa = 4.99PEE7 pKa = 3.96WSWIAACPCCHH18 pKa = 7.32DD19 pKa = 5.99SIDD22 pKa = 3.81QYY24 pKa = 11.83DD25 pKa = 3.9EE26 pKa = 4.05NVEE29 pKa = 4.16EE30 pKa = 4.76EE31 pKa = 4.48GSGDD35 pKa = 4.22DD36 pKa = 5.83SIVTCDD42 pKa = 2.93GCGEE46 pKa = 4.09VFIVSMKK53 pKa = 10.67

Molecular weight: 5.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1LJC6|A0A0K1LJC6_9CAUD Uncharacterized protein OS=Vibrio phage H188 OX=1673892 PE=4 SV=1
MM1 pKa = 7.75EE2 pKa = 6.36IDD4 pKa = 3.63TKK6 pKa = 8.56QLRR9 pKa = 11.84QYY11 pKa = 9.13EE12 pKa = 4.57LKK14 pKa = 10.66LLRR17 pKa = 11.84ANKK20 pKa = 9.25TGFKK24 pKa = 10.11IATEE28 pKa = 3.86QTLNDD33 pKa = 3.75LAFEE37 pKa = 4.58ARR39 pKa = 11.84TQARR43 pKa = 11.84EE44 pKa = 3.73QIEE47 pKa = 3.91RR48 pKa = 11.84DD49 pKa = 3.29FTVRR53 pKa = 11.84NKK55 pKa = 7.72WTLAPRR61 pKa = 11.84NNPIDD66 pKa = 3.4KK67 pKa = 10.39AKK69 pKa = 10.23RR70 pKa = 11.84GKK72 pKa = 9.57PFAEE76 pKa = 4.67FGSTLDD82 pKa = 3.44YY83 pKa = 10.28MRR85 pKa = 11.84KK86 pKa = 8.92QEE88 pKa = 4.53EE89 pKa = 4.47GLISQPDD96 pKa = 3.8RR97 pKa = 11.84FTGAVAIPTPVASGEE112 pKa = 4.29RR113 pKa = 11.84KK114 pKa = 10.02GGAQGKK120 pKa = 5.72TIRR123 pKa = 11.84RR124 pKa = 11.84KK125 pKa = 9.37PVRR128 pKa = 11.84KK129 pKa = 8.81PNRR132 pKa = 11.84RR133 pKa = 11.84NMLKK137 pKa = 9.47MPSKK141 pKa = 10.46RR142 pKa = 11.84MKK144 pKa = 9.57EE145 pKa = 3.84LPRR148 pKa = 11.84RR149 pKa = 11.84EE150 pKa = 3.76RR151 pKa = 11.84NAALIQEE158 pKa = 5.38AIRR161 pKa = 11.84SKK163 pKa = 10.79RR164 pKa = 11.84RR165 pKa = 11.84FIEE168 pKa = 3.86LEE170 pKa = 3.99RR171 pKa = 11.84NGEE174 pKa = 4.17TNIFRR179 pKa = 11.84VKK181 pKa = 10.48GNKK184 pKa = 8.86KK185 pKa = 10.02RR186 pKa = 11.84YY187 pKa = 9.05QLDD190 pKa = 3.23RR191 pKa = 11.84LYY193 pKa = 9.91KK194 pKa = 7.97TEE196 pKa = 3.66QRR198 pKa = 11.84AVISKK203 pKa = 7.93PKK205 pKa = 9.55PWLAPATKK213 pKa = 9.75KK214 pKa = 10.62AYY216 pKa = 8.42EE217 pKa = 3.97QAPRR221 pKa = 11.84FYY223 pKa = 10.25MNRR226 pKa = 11.84LDD228 pKa = 3.9YY229 pKa = 10.51QIKK232 pKa = 10.52RR233 pKa = 11.84MMRR236 pKa = 11.84KK237 pKa = 9.16

Molecular weight: 27.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

15315

42

752

201.5

22.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.077 ± 0.335

1.404 ± 0.162

6.373 ± 0.19

6.53 ± 0.331

3.833 ± 0.18

7.476 ± 0.305

1.75 ± 0.125

6.229 ± 0.203

6.863 ± 0.369

7.307 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.71 ± 0.172

5.367 ± 0.21

3.754 ± 0.254

3.807 ± 0.214

4.107 ± 0.246

6.817 ± 0.293

6.373 ± 0.296

6.843 ± 0.267

1.28 ± 0.124

3.102 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski