Tortoise microvirus 21

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W5X2|A0A4P8W5X2_9VIRU Nonstructural protein OS=Tortoise microvirus 21 OX=2583123 PE=4 SV=1
MM1 pKa = 6.96TGYY4 pKa = 10.01VRR6 pKa = 11.84RR7 pKa = 11.84LTTVEE12 pKa = 4.34LKK14 pKa = 10.84EE15 pKa = 4.01GDD17 pKa = 3.13IPYY20 pKa = 10.33YY21 pKa = 10.69FSTHH25 pKa = 5.74YY26 pKa = 11.33DD27 pKa = 2.52WMQRR31 pKa = 11.84EE32 pKa = 4.39GEE34 pKa = 4.33INCGEE39 pKa = 4.42TIVDD43 pKa = 3.53PSGYY47 pKa = 9.73IPAKK51 pKa = 10.49NRR53 pKa = 11.84IEE55 pKa = 4.01NLLRR59 pKa = 11.84AGEE62 pKa = 4.06RR63 pKa = 11.84LDD65 pKa = 3.25AYY67 pKa = 10.56RR68 pKa = 11.84RR69 pKa = 11.84EE70 pKa = 4.16LYY72 pKa = 10.4DD73 pKa = 3.54YY74 pKa = 11.26GADD77 pKa = 3.89DD78 pKa = 4.8EE79 pKa = 6.07DD80 pKa = 4.21SGFYY84 pKa = 10.81DD85 pKa = 3.76PTRR88 pKa = 11.84DD89 pKa = 3.76PGFDD93 pKa = 3.37PADD96 pKa = 3.36ASKK99 pKa = 10.78INRR102 pKa = 11.84EE103 pKa = 3.16AMARR107 pKa = 11.84VRR109 pKa = 11.84SAQEE113 pKa = 3.51AASVKK118 pKa = 10.27QDD120 pKa = 3.25VEE122 pKa = 4.36AQSEE126 pKa = 4.5APTASNSVSKK136 pKa = 9.79EE137 pKa = 3.71QPTDD141 pKa = 2.72AKK143 pKa = 10.2MNVPGASS150 pKa = 3.09

Molecular weight: 16.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1FVX2|A0A4V1FVX2_9VIRU Replication initiation protein OS=Tortoise microvirus 21 OX=2583123 PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84CTSPLTFGQSVEE14 pKa = 4.07NPLGVKK20 pKa = 9.82IPCGRR25 pKa = 11.84CMACRR30 pKa = 11.84INKK33 pKa = 7.58RR34 pKa = 11.84TEE36 pKa = 3.23WCVRR40 pKa = 11.84LLHH43 pKa = 6.19EE44 pKa = 5.35LEE46 pKa = 4.37YY47 pKa = 9.86WDD49 pKa = 4.79HH50 pKa = 6.31KK51 pKa = 11.43ASFITLTYY59 pKa = 10.89DD60 pKa = 3.64DD61 pKa = 5.57AYY63 pKa = 11.19LHH65 pKa = 6.86LSGLVKK71 pKa = 10.19RR72 pKa = 11.84DD73 pKa = 3.07LQTFFKK79 pKa = 10.59RR80 pKa = 11.84LRR82 pKa = 11.84KK83 pKa = 9.22RR84 pKa = 11.84YY85 pKa = 10.02KK86 pKa = 9.85DD87 pKa = 3.0IKK89 pKa = 8.9YY90 pKa = 10.01YY91 pKa = 10.99AVGEE95 pKa = 4.15YY96 pKa = 10.83GEE98 pKa = 4.66EE99 pKa = 3.86NLRR102 pKa = 11.84PHH104 pKa = 5.5YY105 pKa = 10.0HH106 pKa = 6.76AIVFGFKK113 pKa = 10.67PNDD116 pKa = 3.3LYY118 pKa = 11.11FQEE121 pKa = 4.5EE122 pKa = 4.18SRR124 pKa = 11.84GRR126 pKa = 11.84KK127 pKa = 9.26RR128 pKa = 11.84YY129 pKa = 7.57TSKK132 pKa = 10.78EE133 pKa = 3.59LDD135 pKa = 4.22SIWKK139 pKa = 9.86LGRR142 pKa = 11.84TNVGVVEE149 pKa = 4.82PDD151 pKa = 3.13TVQYY155 pKa = 10.35VCGYY159 pKa = 8.18VVKK162 pKa = 10.87KK163 pKa = 10.89LGGDD167 pKa = 3.82LANYY171 pKa = 10.04VYY173 pKa = 10.71GPKK176 pKa = 9.57QRR178 pKa = 11.84PFALISKK185 pKa = 9.67GLGLRR190 pKa = 11.84HH191 pKa = 6.02VLDD194 pKa = 3.81NKK196 pKa = 10.4EE197 pKa = 4.28KK198 pKa = 8.78YY199 pKa = 9.36QEE201 pKa = 4.02NPEE204 pKa = 4.31VIRR207 pKa = 11.84KK208 pKa = 8.23GKK210 pKa = 10.05RR211 pKa = 11.84LALPRR216 pKa = 11.84YY217 pKa = 8.33YY218 pKa = 10.05RR219 pKa = 11.84VKK221 pKa = 11.2AEE223 pKa = 3.9VNPIKK228 pKa = 10.57IEE230 pKa = 4.12GASLKK235 pKa = 10.68SQVKK239 pKa = 9.53YY240 pKa = 10.98AEE242 pKa = 4.26EE243 pKa = 4.37LEE245 pKa = 4.52KK246 pKa = 11.01SASQTFQKK254 pKa = 10.63QNEE257 pKa = 3.99QRR259 pKa = 11.84EE260 pKa = 4.5KK261 pKa = 10.57NLQSRR266 pKa = 11.84SDD268 pKa = 3.56HH269 pKa = 5.69YY270 pKa = 11.5EE271 pKa = 3.64KK272 pKa = 11.1

Molecular weight: 31.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1377

75

520

229.5

26.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.681 ± 1.016

1.162 ± 0.421

5.882 ± 0.551

7.262 ± 0.705

3.922 ± 0.591

6.391 ± 0.423

1.598 ± 0.251

4.648 ± 0.48

5.519 ± 1.255

7.407 ± 0.62

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.195 ± 0.805

4.866 ± 0.473

4.866 ± 1.061

5.374 ± 1.099

7.117 ± 0.52

5.955 ± 0.252

4.503 ± 0.209

7.117 ± 0.579

1.743 ± 0.45

4.793 ± 0.888
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski