Microbacterium phage PhriedRice

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Eekayvirinae; Akonivirus; unclassified Akonivirus

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6T5C0|A0A5J6T5C0_9CAUD DNA primase OS=Microbacterium phage PhriedRice OX=2652407 GN=3 PE=4 SV=1
MM1 pKa = 8.12AYY3 pKa = 10.2RR4 pKa = 11.84IVTEE8 pKa = 4.09VEE10 pKa = 4.01VAEE13 pKa = 4.49GTDD16 pKa = 3.34AVGISSVLSYY26 pKa = 11.31VLNTSLQGVPGVTSVSITEE45 pKa = 3.87LTAIEE50 pKa = 4.47EE51 pKa = 4.75GNNNAA56 pKa = 4.87

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6T5B8|A0A5J6T5B8_9CAUD Uncharacterized protein OS=Microbacterium phage PhriedRice OX=2652407 GN=39 PE=4 SV=1
MM1 pKa = 7.34MEE3 pKa = 4.26PLIFLGLLIVVGLTIVAIADD23 pKa = 3.16IVKK26 pKa = 9.84YY27 pKa = 7.52EE28 pKa = 3.59RR29 pKa = 11.84RR30 pKa = 11.84MRR32 pKa = 11.84RR33 pKa = 11.84VNNTINSLEE42 pKa = 4.17RR43 pKa = 11.84GRR45 pKa = 11.84GRR47 pKa = 11.84HH48 pKa = 5.58SEE50 pKa = 3.85

Molecular weight: 5.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

17049

38

4489

304.4

33.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.525 ± 0.396

0.463 ± 0.145

6.634 ± 0.211

6.622 ± 0.375

3.232 ± 0.115

7.484 ± 0.325

1.666 ± 0.164

4.962 ± 0.173

4.364 ± 0.379

8.288 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.528 ± 0.123

4.663 ± 0.193

4.552 ± 0.205

4.557 ± 0.307

5.953 ± 0.336

6.188 ± 0.285

6.827 ± 0.277

6.522 ± 0.255

1.842 ± 0.11

3.126 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski